Abstract：
:The rapidity of the optional 90-min differentiation of Naegleria gruberi from amoebae to flagellates suggests the possibility of a free-running cascade of events from initiating stimulus through gene expression to organelle assembly and cell morphogenesis. Instead our experiments reveal two points early in the differentiation at which the strength of the inducing stimulus is reevaluated by the cells. Two new physical start signals for differentiation, temperature downshift (DeltaT) and mechanical agitation, are shown to regulate differentiation synergistically with each other and with previously defined signals. A DeltaT of -10 degrees C induces complete differentiation directly in the growth environment, whereas smaller DeltaTs initiate differentiation and allow it to progress for a short time, after which the cells "hold" for up to 4 h, awaiting a stimulus to continue differentiation. Our work defines two "holdpoints," optional points in development where progress can stop, awaiting a suitable signal, while cells retain whatever intermediates represent progress. We propose that such holdpoints, which can be detected in this system because of the temporal reproducibility of the differentiation, are likely to be found in other differentiating cells.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Fulton C,Lai EYdoi
10.1006/excr.1998.4121subject
Has Abstractpub_date
1998-08-01 00:00:00pages
429-38issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(98)94121-0journal_volume
242pub_type
杂志文章abstract：:Apoptosis is characterized by typical morphological changes and most frequently fragmentation of DNA into oligonucleosome-size fragments. In order to investigate whether an alteration in the mechanisms involved in the process of apoptosis could contribute to cellular resistance, induction of apoptosis was studied in a...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1148
更新日期：1995-05-01 00:00:00
abstract：:We have shown previously that there is a good correlation between the degree of microtubule disassembly by methylmercury (MeHg) and the extent of inhibition of DNA replication in Concanavalin A (Con A)-stimulated mouse splenic lymphocytes. The purpose of this study was to determine if these two events are causally rel...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90383-6
更新日期：1991-08-01 00:00:00
abstract：:Nucleoside diphosphate (NDP) kinases, responsible for the synthesis of nucleoside triphosphates and produced by the nm23 genes, are involved in numerous regulatory processes associated with proliferation, development, and differentiation. Their possible role in providing the GTP/ATP required for sperm function is unkn...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00268-4
更新日期：2003-10-01 00:00:00
abstract：:Cisplatin resistance in cancer cells is due to a pleiotropic phenotype transition that allows cells to resist cell death. miRNAs have been shown to be reliable markers of phenotype, critical in cell differentiation, and dysregulated in cancer and other pathologies. Here we investigate the influence of miRNA on cisplat...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.10.012
更新日期：2013-02-15 00:00:00
abstract：:The stimulatory heterotrimeric Gs protein alpha subunit (Gsα) is a ubiquitous guanine nucleotide-binding protein that regulates the intracellular cAMP signaling pathway and consequently participates in a wide range of biological events. In the reproductive system, despite Gsα being associated with oocyte meiotic arres...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.07.023
更新日期：2018-09-15 00:00:00
abstract：:It is generally accepted that Friedreich's ataxia (FRDA) is caused by a deficiency in frataxin expression, a mitochondrial protein involved in iron homeostasis, which mainly affects the brain, dorsal root ganglia of the spinal cord, heart and in certain cases the pancreas. However, there is little knowledge as to othe...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2015.04.013
更新日期：2015-07-01 00:00:00
abstract：:Quiescence, self-renewal, lineage commitment and differentiation of hematopoietic stem cells (HSCs) towards fully mature blood cells are a complex process that involves both intrinsic and extrinsic signals. During steady-state conditions, most hematopoietic signals are provided by various resident cells inside the bon...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2014.09.016
更新日期：2014-12-10 00:00:00
abstract：:Regions rich in 5-methylcytosine were localized in male metaphase chromosomes of the Indian muntjac deer (Muntiakus muntjak). Chromosomes were ultraviolet irradiated and subsequently photooxidized in the presence of methylene blue to induce maximum DNA denaturation. Following treatment with anti 5-methylcytosine antib...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90289-6
更新日期：1983-08-01 00:00:00
abstract：:Stem cells with the ability to differentiate in specialized cell types can be extracted from a wide array of adult tissues including skeletal muscle. Here we have analyzed a population of cells isolated from skeletal muscle on the basis of their poor adherence on uncoated or collagen-coated dishes that show multi-line...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.01.009
更新日期：2008-04-01 00:00:00
abstract：:Human neutrophils developed long thin tubulovesicular extensions (cytonemes) upon adhesion to fibronectin-coated substrata, when spreading was blocked. We observed extension formation when neutrophils were plated to fibronectin-coated substrata in Na(+)-free extracellular medium or in the presence of drugs capable of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5227
更新日期：2001-06-10 00:00:00
abstract：:In this study we demonstrated that neural rosettes derived from human ES cells can give rise either to neural crest precursors, following expansion in presence of bFGF and EGF, or to dopaminergic precursors after exposure to ventralizing factors Shh and FGF8. Both regionalised precursors are capable of extensive proli...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.02.013
更新日期：2010-04-15 00:00:00
abstract：:Invasion processes underlie or accompany several pathological processes but only a limited number of high-throughput capable phenotypic models exist to test anti-invasive compounds in vitro. We here evaluated 3D co-cultures as a high-content phenotypic screening system for fibrotic invasive processes. 3D multicellular...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2015.10.003
更新日期：2015-11-15 00:00:00
abstract：:Lysosomal cysteine proteinase cathepsin B is implicated in remodeling the extracellular matrix, a crucial step in the process of tumor cell invasion. In this study the contributions of intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT,...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(02)00055-1
更新日期：2003-02-15 00:00:00
abstract：:Contraction of 3D collagen matrices by fibroblasts frequently is used as an in vitro model of wound closure. Different iterations of the model - all conventionally referred to as "contraction" - involve different morphological patterns. During floating matrix contraction, cells initially are round without stress fiber...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.04.013
更新日期：2014-08-15 00:00:00
abstract：:Alexander disease (AxD) is a rare neurodegenerative disorder characterized by large cytoplasmic aggregates in astrocytes and myelin abnormalities and caused by dominant mutations in the gene encoding glial fibrillary acidic protein (GFAP), the main intermediate filament protein in astrocytes. We tested the effects of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.04.035
更新日期：2007-08-01 00:00:00
abstract：:Despite great progress for two decades in microRNAs (miRNAs), the direct regulation of host gene by intragenic (mostly intronic) miRNA is conceptually plausible but evidence-limited. Here, we report that intronic miR-932 could target its host gene via binding with coding sequence (CDS) region rather than regular 3'UTR...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2016.01.017
更新日期：2016-06-10 00:00:00
abstract：:Murine embryonal carcinoma F9 cells can be induced to differentiate by 2-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC). The differentiated phenotype is similar to that of retinoic acid (RA)-treated F9 cells. In contrast to F9 cells the differentiated cells secrete plasminog...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90276-9
更新日期：1985-01-01 00:00:00
abstract：:The binding of actinomycin D (actD) to fixed human metaphase chromosomes was studied by using autoradiography with [3H]actD and indirect immunofluorescence with a specific anti-actD antibody. At concentrations of 0.01 and 0.1 micrograms/ml there was a uniform distribution of drug along the chromosomes as observed by b...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90373-2
更新日期：1984-03-01 00:00:00
abstract：:Human lymphocytes were either exposed to X-irradiation (25 to 200 rads) or treated with H2O2 (9.1 to 291 microM) at 4 degrees C and the extent of DNA migration was measured using a single-cell microgel electrophoresis technique under alkaline conditions. Both agents induced a significant increase in DNA migration, beg...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90265-0
更新日期：1988-03-01 00:00:00
abstract：:By using affinity-purified antibodies to H10 and to H1AB the localization of these histones was studied by indirect immunofluorescence in the nuclei of proliferating (EAT and uninduced Friend cells) and of differentiating (induced Friend cells) cell populations. While with H1AB antibodies a bright fluorescence all ove...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90019-5
更新日期：1988-07-01 00:00:00
abstract：:Tpr is a 267-kDa protein of unknown function recently identified as a constitutive component of the nuclear pore complex (NPC2)-attached intranuclear filaments. Secondary structure predictions suggest that the protein is divided into a large, coiled-coil forming aminoterminal domain and a shorter, highly acidic carbox...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4246
更新日期：1998-11-25 00:00:00
abstract：:Zinc is found in a variety of organisms and has been suggested to be essential for many cellular functions. We report here that zinc ions (Zn2+) have a specific and striking effect on cell differentiation in the simple eukaryote, Dictyostelium discoideum. In its asexual reproduction phase, solitary cells gather to for...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1269
更新日期：1994-09-01 00:00:00
abstract：:Cultured bovine aortic endothelial cells (BAEC) secrete into their medium a growth-promoting factor that stimulates many connective tissue cells in culture. We now report that this growth-promoting activity is due to at least two different proteins which are biochemically separable and immunologically distinct. Cation...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90458-0
更新日期：1984-07-01 00:00:00
abstract：:Lithium exerts neuroprotective actions that involve the inhibition of glycogen synthase kinase-3beta (GSK-3beta). Otherwise, recent studies suggest that sustained GSK-3beta inhibition is a hallmark of tumorigenesis. In this context, the present study was undertaken to examine whether lithium modulated cancer cell sens...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.08.001
更新日期：2004-11-01 00:00:00
abstract：:Alternative splicing is an important mechanism that can disrupt cell cycle control resulting in tumorigenesis. Although many alterations of Transforming Growth Factor Beta (TGFβ) signaling are reported in cancers, the role of splice aberrations in destabilizing this signaling is the least understood mechanism. In this...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.05.004
更新日期：2014-09-10 00:00:00
abstract：:Growth factor activity was partially purified from mouse liver plasma membranes and its growth-stimulatory action on cultured mouse fibroblasts was studied. The plasma membrane-associated growth factor (PMGF) was unable to support the proliferation of mouse fibroblasts in monolayer when added as the sole source of gro...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90126-1
更新日期：1987-05-01 00:00:00
abstract：:The bone-related transcription factor Runx2 (Cbfa1) has been extensively shown to regulate osteoblast differentiation and function. Recent studies demonstrate that Runx2 is also a positive regulator of chondrocyte maturation and vascular invasion in cartilage. Runx2 activity can be modulated in several ways, including...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.05.025
更新日期：2004-09-10 00:00:00
abstract：:CUB domains are most exclusively found in secreted proteins and in a few transmembrane proteins. These domains are approximately 110 amino acids long and have four conserved cysteines that form a β-sandwich fold. CUB domains proteins are involved in a wide range of biological functions. We have shown that CUB domains ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.111499
更新日期：2019-10-01 00:00:00
abstract：:The intracellular ATP concentration decides on the onset of either apoptosis or necrosis in Jurkat cells exposed to death stimuli. Bcl-2 can block apoptotic demise, which occurs preferably under conditions of high cellular ATP levels. Here, we investigated the effects of Bcl-2 on the necrotic type of cell demise that ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.5059
更新日期：2001-01-01 00:00:00
abstract：:We have isolated a monoclonal antibody, P4B2, which localizes to multiple anchorage junctions, namely, a subset of focal adhesions, the Z-disk of muscle, and neuromuscular junctions. Immunopurification of the antigen to this antibody from chicken brain tissue yielded a complex of three prominent proteins with mobiliti...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4155
更新日期：1998-09-15 00:00:00
