Immunopurification of a sarcomeric junctional protein complex containing GAPDH.

abstract：:Transforming growth factor beta regulates many biological processes including cell motility and invasion. Podosomes are specialized F-actin rich structures found in normal cells, such as osteoclasts and macrophages. Tumor cells often form related structures called invadopodia that are thought to promote invasion and m...

journal_title：Experimental cell research

authors： Mandal S,Johnson KR,Wheelock MJ

更新日期：2008-11-15 00:00:00

abstract：:An established melanoma cell line (MM96L) was transfected with selectable plasmid constructs encoding either whole SV40 large T antigen, or beta-galactosidase fusions with the retinoblastoma protein (Rb)-binding region of SV40 large T antigen and a nonbinding mutant derivative of it. Both of the beta-galactosidase fus...

journal_title：Experimental cell research

authors： Khan S,Collins RJ,Dunn IS

更新日期：1996-02-25 00:00:00

abstract：:Chitosan oligosaccharides (COS), linear polymers of N-acetyl-D-glucosamine and deacetylated glucosamine, exhibit diverse pharmacological effects such as antimicrobial, antitumor, antioxidant and anti-inflammatory activities. Here, we explored their hypocholesterolemic effects in vivo and the molecular mechanisms of CO...

journal_title：Experimental cell research

authors： Yang X,Zhang J,Chen L,Wu Q,Yu C

更新日期：2018-05-15 00:00:00

abstract：:Hepatitis B virus (HBV) causes acute and chronic hepatitis, and is one of the major causes of cirrhosis and hepatocellular carcinoma. Accumulating evidence suggests that inflammation is the key factor for liver cirrhosis and hepatocellular carcinoma. MicroRNAs play important roles in many biological processes. Here, w...

journal_title：Experimental cell research

authors： Wu A,Chen H,Xu C,Zhou J,Chen S,Shi Y,Xu J,Gan J,Zhang J

更新日期：2016-11-15 00:00:00

abstract：:The activation of cdc2/cyclin B is the trigger for entry into mitosis. The mechanism of cdc2/cyclin B activation is complex, but the final step is the dephosphorylation of the Thr14 and Tyr15 residues on the cdc2 subunit, catalyzed by a member of the Cdc25 family of phosphatases. Cdc2/cyclin B1 accumulates at the cent...

journal_title：Experimental cell research

authors： De Souza CP,Ellem KA,Gabrielli BG

更新日期：2000-05-25 00:00:00

abstract：:Tumor cell adhesion within host organ microvasculature, its stabilization and invasion into host organ parenchyma appear to be important steps during formation of distant metastasis. These interactions of circulating tumor cells with the host organs occur in the presence of fluid shear forces and soluble and cellular ...

journal_title：Experimental cell research

authors： Korb T,Schlüter K,Enns A,Spiegel HU,Senninger N,Nicolson GL,Haier J

更新日期：2004-09-10 00:00:00

abstract：:We have studied the morphological alterations of the lysosomal compartment in rat hepatocytes following intraperitoneal administration of leupeptin, using electron microscopy and cytochemical techniques. At 30 min after the injection, autophagic vacuoles (autophagosomes and autolysosomes), containing cytoplasmic organ...

journal_title：Experimental cell research

authors： Ishikawa T,Furuno K,Kato K

更新日期：1983-03-01 00:00:00

abstract：:Different extracellular matrix (ECM) molecules, when presented to hippocampal neurons in culture in a substrate-bound form, exert strikingly similar effects on the establishment of neuronal polarity, i.e., the growth of axon-like major neurites is favored, whereas extension of dendrite-like minor neurites is inhibited...

journal_title：Experimental cell research

authors： Lochter A,Schachner M

更新日期：1997-08-25 00:00:00

abstract：:The phenotypic transition of smooth muscle cells (SMC) from a contractile to a synthetic state appears to be an early event in the pathogenesis of atherosclerosis. We examined the effects of extracellular matrix components on the phenotypic modulation of rabbit arterial SMC in primary culture by flow cytometry. The re...

journal_title：Experimental cell research

authors： Yamamoto M,Yamamoto K,Noumura T

更新日期：1993-01-01 00:00:00

abstract：BACKGROUND:Metastatic and/or recurrent breast carcinomas are leading causes of cancer-related death worldwide. Breast cancer stem cells (BCSCs) have been implicated in cancer metastases and progression, thus, the need for the discovery and development of effective BCSCs-specific therapies against metastatic and triple ...

journal_title：Experimental cell research

authors： Chen JH,Kuo KT,Bamodu OA,Lin YC,Yang RB,Yeh CT,Chao TY

更新日期：2018-09-15 00:00:00

abstract：:Lysosomal cysteine proteinase cathepsin B is implicated in remodeling the extracellular matrix, a crucial step in the process of tumor cell invasion. In this study the contributions of intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT,...

journal_title：Experimental cell research

authors： Premzl A,Zavasnik-Bergant V,Turk V,Kos J

更新日期：2003-02-15 00:00:00

abstract：:Cytoplasts derived from senescent and quiescent human diploid cells inhibit DNA synthesis initiation when fused with cells capable of proliferation. When the cytoplasts were subjected to a variety of conditions (trypsin and cycloheximide treatment and growth on fibronectin), this inhibitory activity was lost, suggesti...

journal_title：Experimental cell research

authors： Pereira-Smith OM,Fisher SF,Smith JR

更新日期：1985-10-01 00:00:00

abstract：:Autosomal dominant polycystic kidney disease (ADPKD), a hereditary renal disease caused by mutations in PKD1 (85%) or PKD2 (15%), is characterized by the development of gradually enlarging multiple renal cysts and progressive renal failure. Polycystin-1 (PC1), PKD1 gene product, is an integral membrane glycoprotein wh...

journal_title：Experimental cell research

authors： Kim H,Kang AY,Ko AR,Park HC,So I,Park JH,Cheong HI,Hwang YH,Ahn C

更新日期：2014-01-01 00:00:00

abstract：:We have evaluated the importance of the Ser/Thr protein phosphorylation and dephosphorylation for chondrogenesis in high-density chicken limb bud mesenchymal cell cultures (HDCs) by using H89, a cell-permeable protein kinase inhibitor, and okadaic acid (OA), a phosphoprotein phosphatase (PP)-specific inhibitor molecul...

journal_title：Experimental cell research

authors： Zákány R,Szucs K,Bakó E,Felszeghy S,Czifra G,Bíró T,Módis L,Gergely P

更新日期：2002-04-15 00:00:00

abstract：:Integrin-linked kinase (ILK) is a multifunctional serine/threonine kinase. Accumulating evidences suggest that ILK are involved in cell-matrix interactions, cell proliferation, invasion, migration, angiogenesis and Epithelial-mesenchymal transition (EMT). However, the underlying mechanisms remain largely unknown. EMT ...

journal_title：Experimental cell research

authors： Xing Y,Qi J,Deng S,Wang C,Zhang L,Chen J

更新日期：2013-08-01 00:00:00

abstract：:The highest activity of poly(ADP-ribose) synthetase was found in the testis among several rat tissues tested. Subcellular fractionation of the testis demonstrated that the synthetase was localized primarily in the nucleus and partially in the microsomal-ribosomal fraction. This result was confirmed by immunocytochemic...

journal_title：Experimental cell research

authors： Concha II,Figueroa J,Concha MI,Ueda K,Burzio LO

更新日期：1989-02-01 00:00:00

abstract：:The von Hippel-Lindau (VHL) protein protects microtubules (MTs) from destabilization by nocodazole treatment. Based on this fixed-cell assay with static end points, VHL has been reported to directly stabilize the MT cytoskeleton. To investigate the dynamic changes in MTs induced by VHL in living cells, we measured the...

journal_title：Experimental cell research

authors： Lolkema MP,Mehra N,Jorna AS,van Beest M,Giles RH,Voest EE

更新日期：2004-12-10 00:00:00

abstract：:Human lymphocytes were either exposed to X-irradiation (25 to 200 rads) or treated with H2O2 (9.1 to 291 microM) at 4 degrees C and the extent of DNA migration was measured using a single-cell microgel electrophoresis technique under alkaline conditions. Both agents induced a significant increase in DNA migration, beg...

journal_title：Experimental cell research

authors： Singh NP,McCoy MT,Tice RR,Schneider EL

更新日期：1988-03-01 00:00:00

abstract：:In this study, a somatic cell genetic approach was used to study the regulation of liversolidusbonesoliduskidney alkaline phosphatase (ALPL) gene expression in osteoblasts. ALPL plays an important role in skeletal mineralization and serves as a good index of bone formation. A series of intertypic hybrids constructed b...

journal_title：Experimental cell research

authors： Johnson-Pais TL,Leach RJ

更新日期：1996-07-10 00:00:00

abstract：:dNTP pools are quite low in immature oocytes of the starfish, expand during the 1-methyladenine-induced maturational process and thereafter reach a maximal level (approx. 35, 20, 15 and 5 fmoles/egg for dTTP, dCTP, dATP and dGTP, respectively) which is maintained in overmatured eggs. Maturing oocytes were inseminated ...

journal_title：Experimental cell research

authors： Nagano H,Okano K,Ikegami S

更新日期：1983-04-15 00:00:00

abstract：:Monoclonal antibodies binding to different domains of nucleolin have been used to localize nucleolin in tissue culture cells of Xenopus laevis. The monoclonal antibody b6-6E7 binds to an epitope in the N-terminal domain, which contains arrays of phosphorylation consensus sites. This monoclonal antibody binds to nucleo...

journal_title：Experimental cell research

authors： Schwab MS,Gossweiler U,Dreyer C

更新日期：1998-03-15 00:00:00

abstract：:Hispidin, a phenolic compound from Phellinus linteus (a medicinal mushroom), has been shown to possess strong anti-oxidant, anti-cancer, anti-diabetic, and anti-dementia properties. However, the cardioprotective efficacy of hispidin has not yet been investigated. In the present study, we investigated the protective ef...

journal_title：Experimental cell research

authors： Kim DE,Kim B,Shin HS,Kwon HJ,Park ES

更新日期：2014-10-01 00:00:00

abstract：:Over the last two decades molecular studies of inherited tumor syndromes that are associated with the development of kidney cancer have led to the identification of genes and biochemical pathways, which play key roles in the malignant transformation of renal epithelial cells. Some of these findings have broad biologic...

journal_title：Experimental cell research

authors： Haase VH

更新日期：2012-05-15 00:00:00

abstract：:The protein kinase mammalian target of rapamycin (mTOR) is a central regulator of cell proliferation and growth, with the ribosomal subunit S6 kinase 1 (S6K1) as one of the key downstream signaling effectors. A critical role of mTOR signaling in skeletal muscle differentiation has been identified recently, and an unus...

journal_title：Experimental cell research

authors： Park IH,Erbay E,Nuzzi P,Chen J

更新日期：2005-09-10 00:00:00

abstract：:VEGF is a major inducer of angiogenesis. However, the homing role of VEGF for cardiac stem cells (CSCs) is unclear. In in vitro experiments, CSCs were isolated from the rat hearts, and a cellular migration assay was performed using a 24-well transwell system. VEGF induced CSC migration in a concentration-dependent man...

journal_title：Experimental cell research

authors： Tang J,Wang J,Kong X,Yang J,Guo L,Zheng F,Zhang L,Huang Y,Wan Y

更新日期：2009-12-10 00:00:00

abstract：:Defects in the regulation of programmed cell death play a fundamental role in the development of neoplasia and neurological disorders, both of which are linked to the human T-cell leukemia/lymphoma virus type 1 (HTLV-1) infection. We previously showed that the HTLV-1 Tax protein protects from apoptosis induced by seru...

journal_title：Experimental cell research

authors： Trevisan R,Daprai L,Paloschi L,Vajente N,Chieco-Bianchi L,Saggioro D

更新日期：2006-05-01 00:00:00

abstract：:Previous studies have established that Schwann cells (SC) in culture express an NGF receptor. In this study, cultures of fetal human SC were established from fetal nerves and various light microscopic (LM) and electron microscopic (EM) techniques were used to localize the NGF receptor on the SC. Results indicate that ...

journal_title：Experimental cell research

authors： Assouline JG,Pantazis NJ

更新日期：1989-06-01 00:00:00

abstract：:Rat IPC-81 promyelocytic leukemia cells responded to cAMP analog by undergoing apoptotic cell death both when anchored to fibronectin and when free in the medium. The protein kinase C stimulator 12-O-tetradecanoylphorbol 13-acetate enhanced the anchoring to substratum without impeding cAMP-induced cell death. The immo...

journal_title：Experimental cell research

authors： Vintermyr OK,Gjertsen BT,Lanotte M,Døskeland SO

更新日期：1993-05-01 00:00:00

abstract：:Bone morphogenetic protein-4 (BMP-4) is a member of the transforming growth factor-beta (TGF-beta) supergene family and is characterized by its ability to induce singly de novo cartilage and bone in vivo. The influence of recombinant bone morphogenetic protein-4 and some related members, TGF-beta 1, activin A, and inh...

journal_title：Experimental cell research

authors： Luyten FP,Chen P,Paralkar V,Reddi AH

更新日期：1994-02-01 00:00:00

abstract：:Basic fibroblast growth factor (bFGF) is a mitogen and a survival factor in fibroblasts and endothelial cells. It acts as an angiogenesis factor in breast cancer, but paradoxically inhibits proliferation in several breast cancer cell lines. In this study, we investigated the effects of bFGF on the survival of MCF-7 hu...

journal_title：Experimental cell research

authors： Wang Q,Maloof P,Wang H,Fenig E,Stein D,Nichols G,Denny TN,Yahalom J,Wieder R

更新日期：1998-01-10 00:00:00