Abstract：
:Hepatitis B virus (HBV) causes acute and chronic hepatitis, and is one of the major causes of cirrhosis and hepatocellular carcinoma. Accumulating evidence suggests that inflammation is the key factor for liver cirrhosis and hepatocellular carcinoma. MicroRNAs play important roles in many biological processes. Here, we aim to explore the function of microRNAs in the HBX-induced inflammation. First, microarray experiment showed that HBV+ liver samples expressed higher level of miR-203a compared to HBV- liver samples. To verify these alterations, HBx-coding plasmid was transfected into HepG2 cells to overexpress HBx protein. The real-time PCR results suggested that over-expression of HBx could induce up-regulation of miR-203a. To define how up-regulation of miR-203a can induce liver cells inflammation, we over-expressed miR-203a in HepG2 cells. Annexin V staining and BrdU staining suggested that overexpression of miR-203a significantly increased the cell apoptosis and proliferation, meanwhile, over-expression of miR-203a could lead to a decrease in G0/G1 phase cells and an increase in G2/M phase cells. Some cytokines production including IL-6 and IL-8 were significantly increased, but TGFβ and IFNγ were decreased in miR-203a over-expressed HepG2 cells. Luciferase reporter assay experiments, protein mass-spectrum assay and real-time PCR all together demonstrated that Rap1a was the target gene of miR-203a. Further experiments showed that these alterations were modulated through PI3K/ERK/p38/NFκB pathways. These data suggested that HBV-infection could up-regulate the expression of miR-203a, thus down regulated the expression of Rap1a and affected the PI3K/ERK/p38/NFκB pathways, finally induced the hepatitis inflammation.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Wu A,Chen H,Xu C,Zhou J,Chen S,Shi Y,Xu J,Gan J,Zhang Jdoi
10.1016/j.yexcr.2016.10.016subject
Has Abstractpub_date
2016-11-15 00:00:00pages
191-197issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(16)30343-3journal_volume
349pub_type
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2014-09-10 00:00:00
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journal_title：Experimental cell research
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更新日期：2007-07-15 00:00:00
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pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.01.006
更新日期：2007-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90283-5
更新日期：1983-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.10.009
更新日期：2017-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.12.002
更新日期：2006-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1149
更新日期：1995-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1246
更新日期：1993-09-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1235
更新日期：1994-09-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90480-x
更新日期：1985-06-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Experimental cell research
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更新日期：2013-04-01 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.03.037
更新日期：2004-07-15 00:00:00
abstract：:In the naturally synchronous mitosis of the syncytial plasmodium of Physarum polycephalum, the nucleolus disintegrates in prophase, releasing a large amount of ribosomal RNA. Using biotinylated rDNA probes, we studied by high-resolution in situ hybridization the behavior of this nucleolar RNA throughout mitosis. Our r...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1274
更新日期：1993-10-01 00:00:00
