Abstract:
:Activin A belongs to the superfamily of transforming growth factor beta (TGFβ) and is a critical regulatory cytokine in breast cancer and inflammation. However, the role of activin A in migration of breast cancer cells and immune cells was not well characterized. Here, a microfluidic device was used to examine the effect of activin A on the migration of human breast cancer cell line MDA-MB-231 cells and human blood neutrophils as well as their migratory interaction. We found that activin A promoted the basal migration but impaired epidermal growth factor (EGF)-induced migration of breast cancer cells. By contrast, activin A reduced neutrophil chemotaxis and transendothelial migration to N-Formyl-Met-Leu-Phe (fMLP). Finally, activin A promoted neutrophil chemotaxis to the supernatant from breast cancer cell culture. Collectively, our study revealed the different roles of activin A in regulating the migration of breast cancer cells and neutrophils and their migratory interaction. These findings suggested the potential of activin A as a therapeutic target for inflammation and breast cancers.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Xie D,Liu Z,Wu J,Feng W,Yang K,Deng J,Tian G,Santos S,Cui X,Lin Fdoi
10.1016/j.yexcr.2017.05.003subject
Has Abstractpub_date
2017-08-01 00:00:00pages
107-115issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(17)30267-7journal_volume
357pub_type
杂志文章abstract::Lithium has been found to be a novel inhibitor of the terminal differentiation of Friend murine erythroleukemia cells. A general method for the quantitative analysis of differentiation inhibitors has been developed and used to compare the site of inhibition by lithium with that by vanadate. Lithium inhibits the commit...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(87)90012-7
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abstract::Plasmid 6.4 kbp DNA, 14 kbp DNA, lambda phage particles, all of which contained herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) gene, or IgM molecules, were mixed with erythrocyte membranes and treated with neutral detergent. The transparent mixture was diluted with phosphate-buffered saline (PBS), followed ...
journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.06.029
更新日期:2006-10-15 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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doi:10.1006/excr.1996.0088
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abstract::There is at present a controversy in the literature whether MSCs are susceptible to spontaneous in vitro transformation or not. Several groups have reported spontaneous transformation of MSCs from various species. However, some of these reports were not true transformations and later proven to be due to cross-contamin...
journal_title:Experimental cell research
pub_type: 评论,杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.07.016
更新日期:2018-09-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90366-3
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abstract::We examined the relationship of cellular oncogene c-myc and transferrin receptor (TfR) gene expression to cell proliferation and cell cycle progression during myeloid differentiation in the HL-60 myeloid leukemia cell line. In order to determine levels of mRNA for these genes in HL-60 cells induced to differentiate al...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90202-l
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.01.021
更新日期:2004-06-10 00:00:00
abstract::A novel protein that associates with interphase nucleus and mitotic apparatus (INMAP) was identified by screening HeLa cDNA expression library with an autoimmune serum followed by tandem mass spectrometry. Its complete cDNA sequence of 1.818 kb encodes 343 amino acids with predicted molecular mass of 38.2 kDa and nume...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.01.022
更新日期:2009-04-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4491
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abstract::Epithelial ovarian cancer is the most common form of gynaecological malignancy. This lethal disease is thought to arise in ovarian surface epithelial (OSE) cells. The biology of these cells is not well understood, due to the limited amount of tissue that can be obtained from a single biopsy and their limited life span...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(03)00218-0
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abstract::The mitochondrion depends upon the import of cytosolically synthesized preproteins for most of the proteins that comprise its structural elements and metabolic pathways. Here we have examined the influence of redox conditions on mitochondrial preprotein import and processing by mammalian mitochondria. Paraquat pretrea...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.5096
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abstract::Inhibition of the ligand-activated androgen receptor (AR) by antiandrogens plays an important role in the treatment of various hyperandrogenic disorders including prostate cancer. However, the molecular mechanisms of antiandrogen activity in vivo remain unclear. In this study we analyzed the effects of cyproterone ace...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.5018
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journal_title:Experimental cell research
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doi:10.1006/excr.1994.1055
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abstract::The Rab11 family of small GTPases is composed of three members, Rab11a, Rab11b, and Rab25. While recent work on Rab11a and Rab25 has yielded some insights into their function, Rab11b has received little attention. Therefore, we sought to examine the distribution of endogenous Rab11b in epithelial cells. In rabbit gast...
journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90188-0
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
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更新日期:2019-03-15 00:00:00
abstract::The tumor suppressor protein p16INK4a (inhibitor of CDK4) is one of the most direct links between cell-cycle control and cancer. The p16INK4a gene is frequently inactivated in human tumors, and inheritance of mutant alleles results in susceptibility to several types of cancer. p16INK4a is part of a cell-cycle regulato...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1006/excr.1997.3824
更新日期:1997-11-25 00:00:00
abstract::Human lacrimal gland biopsies were serially incubated in chelating and enzymatic solutions. Viable single cells were evaluated for outgrowth in growth factor enriched medium using the following culture substrates: Matrigel, Type I collagen gel with or without fibroblasts, and plastic. Each epithelial outgrowth was cha...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1300
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.12.015
更新日期:2007-03-10 00:00:00
abstract::Durations of S-phase (Ts) and total cell cycle times (Tc) were measured from the peripheral blood (PB) and bone marrow aspirates (BM) of five patients with acute nonlymphocytic leukemia (ANLL). Intravenous bromodeoxyuridine (BrdU) was used as the first label for S-phase cells and a monoclonal anti-BrdU antibody was us...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(88)90116-4
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