The effects of activin A on the migration of human breast cancer cells and neutrophils and their migratory interaction.


:Activin A belongs to the superfamily of transforming growth factor beta (TGFβ) and is a critical regulatory cytokine in breast cancer and inflammation. However, the role of activin A in migration of breast cancer cells and immune cells was not well characterized. Here, a microfluidic device was used to examine the effect of activin A on the migration of human breast cancer cell line MDA-MB-231 cells and human blood neutrophils as well as their migratory interaction. We found that activin A promoted the basal migration but impaired epidermal growth factor (EGF)-induced migration of breast cancer cells. By contrast, activin A reduced neutrophil chemotaxis and transendothelial migration to N-Formyl-Met-Leu-Phe (fMLP). Finally, activin A promoted neutrophil chemotaxis to the supernatant from breast cancer cell culture. Collectively, our study revealed the different roles of activin A in regulating the migration of breast cancer cells and neutrophils and their migratory interaction. These findings suggested the potential of activin A as a therapeutic target for inflammation and breast cancers.


Exp Cell Res


Xie D,Liu Z,Wu J,Feng W,Yang K,Deng J,Tian G,Santos S,Cui X,Lin F




Has Abstract


2017-08-01 00:00:00














  • The acquired radioresistance in HeLa cells under conditions mimicking hypoxia was attenuated by a decreased expression of HIF subunit genes induced by RNA interference.

    abstract::The cancer cells residing in the hypoxic layer are resistant to radiation and these are ones responsible for cancer recurrence after radiation therapy. One of the reasons why hypoxic cancer cells acquire radioresistance may be attributable to changes in the gene expression profile by the activation of hypoxia inducibl...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Doi N,Ogawa R,Cui ZG,Morii A,Watanabe A,Kanayama S,Yoneda Y,Kondo T

    更新日期:2015-05-01 00:00:00

  • Inhibition of the melanoma cell cycle and regulation at the G1/S transition by 12-O-tetradecanoylphorbol-13-acetate (TPA) by modulation of CDK2 activity.

    abstract::The growth of malignant melanoma cells is inhibited by 12-O-tetradecanoylphorbol-13-acetate (TPA) while the growth of normal melanocytes is stimulated. We previously demonstrated that TPA inhibits the growth of Demel melanoma cells and leads to arrest at both at the G1/S and G2/M cell cycle transitions. To investigate...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Coppock DL,Buffolino P,Kopman C,Nathanson L

    更新日期:1995-11-01 00:00:00

  • The role of peroxisome proliferator-activated receptor-beta/delta in epidermal growth factor-induced HaCaT cell proliferation.

    abstract::Epidermal growth factor (EGF) has been shown to be a potent mitogen for epidermal cells both in vitro and in vivo, thus contributing to the development of an organism. It has recently become clear that peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) expression and activation is involved in the c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Liang P,Jiang B,Yang X,Xiao X,Huang X,Long J,Zhang P,Zhang M,Xiao M,Xie T,Huang X

    更新日期:2008-10-15 00:00:00

  • Organization of early and late replicating DNA in human chromosome territories.

    abstract::It has been suggested that DNA organized into replication foci during S-phase remains stably aggregated in non-S-phase cells and that these stable aggregates provide fundamental units of nuclear or chromosome architecture [C. Meng and R. Berezney (1991) J. Cell Biol. 115, 95a; E. Sparvoli et al. (1994) J. Cell Sci. 10...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zink D,Bornfleth H,Visser A,Cremer C,Cremer T

    更新日期:1999-02-25 00:00:00

  • Disintegrin causes proteolysis of beta-catenin and apoptosis of endothelial cells. Involvement of cell-cell and cell-ECM interactions in regulating cell viability.

    abstract::Disintegrins, the snake venom-derived arginine-glycine-aspartic acid-containing peptides, have been demonstrated to inhibit angiogenesis through induction of endothelial cell apoptosis. However, it is not clear how a disintegrin causes endothelial apoptosis. In this study, we elucidated the action mechanism of disinte...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wu WB,Peng HC,Huang TF

    更新日期:2003-05-15 00:00:00

  • A tubulin identified by a monoclonal antibody is not present in mitotic spindles.

    abstract::A monoclonal antibody, G8, which recognizes a form of tubulin (G8-tubulin) with a novel distribution in Rat-1 cells and Potorous tridactylis kidney (Ptk-2) cells was isolated. G8 labeled the interphase cytoskeleton of Rat-1 fibroblasts but not mitotic spindles or midbodies. G8 also stained a fiber network in some but ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gossels JM,Ingram VM

    更新日期:1989-10-01 00:00:00

  • Antiangiogenesis in cancer therapy--endostatin and its mechanisms of action.

    abstract::The first angiogenesis inhibitors for cancer have now been approved by the F.D.A. in the U.S. and in 28 other countries, including China. The majority of these are monotherapies that block VEGF. However, mutant tumor cells may over time produce redundant angiogenic factors. Therefore, for long-term use in cancer, comb...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Folkman J

    更新日期:2006-03-10 00:00:00

  • NLRP3 inflammasome inhibition attenuates cisplatin-induced renal fibrosis by decreasing oxidative stress and inflammation.

    abstract:BACKGROUND/AIMS:The NOD-like receptor, pyrin domain containing-3 (NLRP3) inflammasome is involved in the progression of chronic kidney disease in several rodent models. Here, we investigated whether a specific inhibitor of NLRP3 inflammasome, MCC950, can attenuate cisplatin-induced renal fibrosis. MATERIALS:Renal fibr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Li S,Lin Q,Shao X,Mou S,Gu L,Wang L,Zhang Z,Shen J,Zhou Y,Qi C,Jin H,Pang H,Ni Z

    更新日期:2019-10-01 00:00:00

  • Analysis of Tax-expressing cell lines generated from HTLV-I tax-transgenic mice: correlation between c-myc overexpression and neoplastic potential.

    abstract::Human T-cell leukemia/lymphotropic virus type I (HTLV-I) infection causes a variety of human diseases, including adult T-cell leukemia/lymphoma. The viral transactivator Tax has been implicated as a key factor in the HTLV-I-induced transformation pathway. To investigate the components of this pathway, we derived fibro...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Saggioro D,D'agostino DM,Chieco-Bianchi L

    更新日期:1999-03-15 00:00:00

  • The over-expression of HAS2, Hyal-2 and CD44 is implicated in the invasiveness of breast cancer.

    abstract::Within tumors there appears to be an intricate balance between hyaluronan (HA) synthesis and degradation where the invading edges display increased HA metabolism. The metabolism of HA has not been characterized in breast cancer cell lines; therefore, this study quantitatively identifies and characterizes the enzymes r...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Udabage L,Brownlee GR,Nilsson SK,Brown TJ

    更新日期:2005-10-15 00:00:00

  • A novel 3D high-content assay identifies compounds that prevent fibroblast invasion into tissue surrogates.

    abstract::Invasion processes underlie or accompany several pathological processes but only a limited number of high-throughput capable phenotypic models exist to test anti-invasive compounds in vitro. We here evaluated 3D co-cultures as a high-content phenotypic screening system for fibrotic invasive processes. 3D multicellular...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wenzel C,Otto S,Prechtl S,Parczyk K,Steigemann P

    更新日期:2015-11-15 00:00:00

  • Disruption of cytoplasmic microtubules by ultraviolet radiation.

    abstract::Ultraviolet (UV) irradiation of cultured human skin fibroblasts causes the disassembly of their microtubules. Using indirect immunofluorescence microscopy, we have now investigated whether damage to the microtubule precursor pool may contribute to the disruption of microtubules. Exposure to polychromatic UV radiation ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zamansky GB,Perrino BA,Chou IN

    更新日期:1991-07-01 00:00:00

  • ISG15 promotes esophageal squamous cell carcinoma tumorigenesis via c-MET/Fyn/β-catenin signaling pathway.

    abstract::Esophageal squamous cell carcinoma (ESCC) is one of the most malignant tumors in China with a poor prognosis. Most ESCC patients were diagnosed at advanced stages, losing the opportunity for surgical excision. Hence, it remains a pressing work to identify biomarkers for early detection, prognosis prediction and target...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yuan H,Zhou W,Yang Y,Xue L,Liu L,Song Y

    更新日期:2018-06-01 00:00:00

  • Formins: processive cappers of growing actin filaments.

    abstract::Taking the advantage of single-molecule imaging, our recent study has revealed surprisingly long processive movement of a Formin protein, mDia1, surfing along with the growing end of actin filaments in living cells. This finding provides direct evidence for the ability of Formins to function as processive cappers that...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Watanabe N,Higashida C

    更新日期:2004-11-15 00:00:00

  • Plasminogen activator inhibitor type-2 (PAI-2) in human keratinocytes regulates pericellular urokinase-type plasminogen activator.

    abstract::Plasminogen activation is observed in the human epidermis during reepithelialization of epidermal defects and under certain pathological conditions. The activation reaction depends on keratinocyte-associated plasminogen activators (PAs), which convert the ubiquitous proenzyme plasminogen into the active trypsin-like s...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Reinartz J,Schaefer B,Bechtel MJ,Kramer MD

    更新日期:1996-02-25 00:00:00

  • Identification of microsomal triglyceride transfer protein in intestinal brush-border membrane.

    abstract::Microsomal triglyceride transfer protein (MTP) is a heterodimeric complex consisting of a unique large 97-kDa protein and the multifunctional 58-kDa protein disulfide isomerase (PDI). It plays an essential role in the assembly of lipoproteins by shuttling lipids between phospholipid membranes. Based on cell fractionat...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Slight I,Bendayan M,Malo C,Delvin E,Lambert M,Levy E

    更新日期:2004-10-15 00:00:00

  • Effects of osmotic shocks on the ultrastructure of different tissues and cell types.

    abstract::This study deals with the effects of hyper- and hypo-osmotic media on the ultrastructure of four different types of cells and tissues: rat pheochromocytoma cells of line PC12, mouse Ehrlich ascites tumor cells, rat kidney cortex and intestine. Application of hyper-osmotic conditions induces in the nuclear compartment ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Delpire E,Duchêne C,Goessens G,Gilles R

    更新日期:1985-09-01 00:00:00

  • Characterization of lamina-bound chromatin in the nuclear shell isolated from HeLa cells.

    abstract::The structure and the polypeptide composition of the nuclear shell isolated from interphase HeLa cells have been investigated and compared to those of the intranuclear material. The isolated nuclear shell contains chromatin superstructures (28-32 nm thick fibres) made of tightly packed nucleosomes that resist low ioni...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Bouvier D,Hubert J,Seve AP,Bouteille M

    更新日期:1985-02-01 00:00:00

  • Transforming growth factor beta stimulates fibroblast-collagen matrix contraction by different mechanisms in mechanically loaded and unloaded matrices.

    abstract::Studies were carried out to test the idea that transforming growth factor beta (TGFbeta) stimulated fibroblast contraction of collagen matrices by different mechanisms depending on mechanical loading on the cells and matrix. Under mechanically unloaded conditions (floating matrices), TGFbeta stimulated contraction dir...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Grinnell F,Ho CH

    更新日期:2002-02-15 00:00:00

  • Sodium arsenite accelerates TRAIL-mediated apoptosis in melanoma cells through upregulation of TRAIL-R1/R2 surface levels and downregulation of cFLIP expression.

    abstract::AP-1/cJun, NF-kappaB and STAT3 transcription factors control expression of numerous genes, which regulate critical cell functions including proliferation, survival and apoptosis. Sodium arsenite is known to suppress both the IKK-NF-kappaB and JAK2-STAT3 signaling pathways and to activate the MAPK/JNK-cJun pathways, th...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ivanov VN,Hei TK

    更新日期:2006-12-10 00:00:00

  • Protein kinase C regulates the phosphorylation and oligomerization of ERM binding phosphoprotein 50.

    abstract::Ezrin-Radixin-Moesin (ERM) binding phosphoprotein 50 (EBP50, a.k.a. NHERF-1) is a scaffold protein essential for the localization and coordinated activity of apical transporters, enzymes and receptors in epithelial cells. EBP50 acts via multiple protein binding interactions, including oligomerization through interacti...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Fouassier L,Nichols MT,Gidey E,McWilliams RR,Robin H,Finnigan C,Howell KE,Housset C,Doctor RB

    更新日期:2005-05-15 00:00:00

  • Fibroblasts maintain a complete endocytic pathway in the presence of lysosomotropic amines.

    abstract::We have investigated the effects of the lysosomotropic amines, ammonium chloride and chloroquine, on the delivery of fluid-phase pinocytic tracers to lysosomes in Chinese hamster ovary (CHO) cells. In preliminary experiments, 15 mM ammonium chloride and 0.1 mM chloroquine were found to be sufficient to give maximal pr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Fritsch JE,Buckmaster MJ,Storrie B

    更新日期:1988-04-01 00:00:00

  • CUB domain-containing protein 1 (CDCP1) binds transforming growth factor beta family members and increase TGF-β1 signaling pathway.

    abstract::CUB domains are most exclusively found in secreted proteins and in a few transmembrane proteins. These domains are approximately 110 amino acids long and have four conserved cysteines that form a β-sandwich fold. CUB domains proteins are involved in a wide range of biological functions. We have shown that CUB domains ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Predes D,Cruz JVR,Abreu JG,Mendes FA

    更新日期:2019-10-01 00:00:00

  • Down-regulation of insulin receptors is related to insulin internalization.

    abstract::In the present study, we have tested the influence of inhibition of endocytosis by hypertonic medium on the regulation of cell surface insulin receptors. We show that active internalization of 125I-insulin is markedly inhibited by hypertonic media and that, in parallel, cell surface invaginations are significantly dim...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Geiger D,Carpentier JL,Gorden P,Orci L

    更新日期:1989-11-01 00:00:00

  • Protein L-isoaspartyl methyltransferase repairs abnormal aspartyl residues accumulated in vivo in type-I collagen and restores cell migration.

    abstract::Abnormal aspartyl residue formation such as L-isoaspartates occurs frequently during aging in long-lived proteins, resulting in the alteration of their structures and biological functions. In this study, we investigated the alteration of aspartyl residues in extracellular matrix (ECM) proteins, type-I collagen and fib...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lanthier J,Desrosiers RR

    更新日期:2004-02-01 00:00:00

  • Hsf1 on a leash - controlling the heat shock response by chaperone titration.

    abstract::Heat shock factor 1 (Hsf1) is an ancient transcription factor that monitors protein homeostasis (proteostasis) and counteracts disturbances by triggering a transcriptional programme known as the heat shock response (HSR). The HSR is transiently activated and upregulates the expression of core proteostasis genes, inclu...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Masser AE,Ciccarelli M,Andréasson C

    更新日期:2020-11-01 00:00:00

  • Fate of a headless vimentin protein in stable cell cultures: soluble and cytoskeletal forms.

    abstract::The non-alpha-helical head domains of cytoskeletal intermediate filaments (IFs) are considered to play an important role in IF assembly and stability. We have investigated the fate of a "headless" mutant vimentin protein in cell types that either lack cytoplasmic IFs or contain preexisting IF networks (keratin and vim...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Andreoli JM,Trevor KT

    更新日期:1994-09-01 00:00:00

  • Protein synthesis in lactating guinea-pig mammary tissue perfused in vitro. I. Radiolabelling of membrane and secretory proteins.

    abstract::A method for the in vitro perfusion of isolated guinea-pig mammary tissue is described that allows the radiolabelling of secretory and membrane proteins. Glands were depleted of methionine, labelled with [35S]methionine for 5 min and perfused with medium containing an excess of unlabelled methionine for varying times....

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mather IH,Jarasch ED,Bruder G,Heid HW,Mepham TB

    更新日期:1984-03-01 00:00:00

  • MiR-223/Pknox1 axis protects mice from CVB3-induced viral myocarditis by modulating macrophage polarization.

    abstract::Macrophage polarization plays a crucial role in regulating myocardial inflammation and injuries of coxsackievirus B3 (CVB3)-induced viral myocarditis (VM). It has been reported that miR-223 is a potent regulator of inflammatory responses that involved in macrophage polarization. However, the functional roles of miR-22...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gou W,Zhang Z,Yang C,Li Y

    更新日期:2018-05-01 00:00:00

  • Regulation of ROCKII membrane localization through its C-terminus.

    abstract::RhoA activated kinases (ROCKs) are potent effectors of RhoA signaling for regulation of the cytoskeleton. ROCKs have been shown to be localized to several different subcellular locations, suggesting that its localization is context specific and regulated. However, the signaling mechanisms that control ROCK localizatio...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kher SS,Worthylake RA

    更新日期:2011-12-10 00:00:00