Proliferation of submesothelial mesenchymal cells during early phase of serosal thickening in the rabbit bladder is accompanied by transient keratin 18 expression.

Abstract:

:Partial outlet obstruction of the rabbit bladder induces serosal thickening and smooth muscle (SM) hypertrophy. Within thickened serosa, submesothelial (mesenchymal) cells differentiate into SM cells after 30 days of obstruction[S. Buoro et al. Lab. Invest. 69, 589-602, 1993]. Here, we show that submesothelial cells transiently express keratin (K) 18 but not K8 soon after obstruction. We investigated a possible relationship between keratin expression and cell proliferation/differentiation in vivo and in vitro. The results of this study indicate that expression of K18 is spatiotemporally related to the pattern of cell proliferation with respect to the localization of an elastic membrane which divides the thickened serosa into an "extrinsic" and an "intrinsic" region. Moreover, K18 is not present in bladder mesenchyma during early development, indicating that its expression in the adult is not attributable to a dedifferentiation process. However, simultaneous K18, K8, and desmoplakin (DP) expression can be induced in normal and thickened serosa upon treatment with bromo-deoxyuridine. Our results indicate that K18 is a marker of proliferating mesenchymal cells in rabbit serosa, whereas the combined expression of K18, K8, and DP might be related to the hypothesized alterations in the stability of gene expression. A model is proposed in which keratin-containing submesothelial cells can act as a "transit" cell phenotype involved in both regenerating mesothelial cells and formation of SM cells.

journal_name

Exp Cell Res

authors

Pampinella F,Roelofs M,Castellucci E,Chiavegato A,Guidolin D,Passerini-Glazel G,Pagano F,Sartore S

doi

10.1006/excr.1996.0088

subject

Has Abstract

pub_date

1996-03-15 00:00:00

pages

327-39

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(96)90088-9

journal_volume

223

pub_type

杂志文章
  • Isolation and immunohistochemical characterization of two developmentally regulated highly insoluble antigens from the membrane of sinusoidal rat liver cells.

    abstract::Using sequential extractions with buffers containing or not containing neutral detergent, two highly insoluble protein components were found in livers of 30-day-old rats. These compounds (molecular weights (MW) 52 900 and 33 200 respectively) were practically absent from livers of young rats (between 5 and 8 postnatal...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(84)90197-6

    authors: Zanetta JP,Bingen A,Dontenwill M,Vincendon G

    更新日期:1984-12-01 00:00:00

  • Dynamic organization of DNA replication in one-cell mouse embryos: relationship to transcriptional activation.

    abstract::We have analyzed the spatial and temporal relationship between transcription and replication sites during the first cell cycle in mouse embryos. Embryos were microinjected with both 5-bromouridine-5'-triphosphate and digoxygenin-11-deoxyuridine-5'-triphosphate to visualize transcription and replication sites respectiv...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1997.3708

    authors: Bouniol-Baly C,Nguyen E,Besombes D,Debey P

    更新日期:1997-10-10 00:00:00

  • The RGD integrin binding site in human L1-CAM is important for nuclear signaling.

    abstract::L1 cell adhesion molecule (L1-CAM) is a transmembrane cell adhesion molecule initially defined as a promigratory molecule in the developing nervous system. L1 is also overexpressed in a variety of human carcinomas and is associated with bad prognosis. In carcinoma cell lines L1 augments cell motility and metastasis, t...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2008.04.004

    authors: Gast D,Riedle S,Kiefel H,Müerköster SS,Schäfer H,Schäfer MK,Altevogt P

    更新日期:2008-08-01 00:00:00

  • Identification of a nuclear export signal in the KSHV latent protein LANA2 mediating its export from the nucleus.

    abstract::LANA2 is a latent protein detected in Kaposi's sarcoma-associated herpesvirus (KSHV)-infected B cells that inhibits p53-dependent transcriptional transactivation and apoptosis and PKR-dependent apoptosis, suggesting an important role in the transforming activity of the virus. It has been reported that LANA2 localizes ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2005.08.022

    authors: Muñoz-Fontela C,Collado M,Rodriguez E,García MA,Alvarez-Barrientos A,Arroyo J,Nombela C,Rivas C

    更新日期:2005-11-15 00:00:00

  • Effects of anti-PM-Scl 100 (Rrp6p exonuclease) antibodies on prenucleolar body dynamics at the end of mitosis.

    abstract::Prenucleolar bodies (PNBs) are transitory structures which serve as building blocks for nucleoli at the transition mitosis/interphase. The assembly of PNBs and their pathway are not clearly understood. To better understand these events, the behavior of the PNB-containing PM-Scl 100 protein was compared with that of ot...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4578

    authors: Fomproix N,Hernandez-Verdun D

    更新日期:1999-09-15 00:00:00

  • Mitotic segregation of the nucleolar ribosomal RNA in Physarum polycephalum.

    abstract::In the naturally synchronous mitosis of the syncytial plasmodium of Physarum polycephalum, the nucleolus disintegrates in prophase, releasing a large amount of ribosomal RNA. Using biotinylated rDNA probes, we studied by high-resolution in situ hybridization the behavior of this nucleolar RNA throughout mitosis. Our r...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1993.1274

    authors: Pierron G,Puvion-Dutilleul F

    更新日期:1993-10-01 00:00:00

  • Murine embryonal carcinoma cell-surface sialyl LeX is present on a novel glycoprotein and on high-molecular-weight lactosaminoglycan.

    abstract::Carbohydrate-specific monoclonal antibodies were used to demonstrate the expression of a new membrane glycoprotein on F9 murine embryonal carcinoma cells. Sialyl LeX was detected using monoclonal antibody FH6 in a sensitive, cell monolayer radioimmunoassay. The antigen codistributed in gel filtration of a crude homoge...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(89)90061-x

    authors: Rosenman SJ,Fenderson BA,Hakomori SI

    更新日期:1989-02-01 00:00:00

  • Cdc6 is a rate-limiting factor for proliferative capacity during HL60 cell differentiation.

    abstract::The DNA replication (or origin) licensing pathway represents a critical step in cell proliferation control downstream of growth signalling pathways. Repression of origin licensing through down-regulation of the MCM licensing factors (Mcm2-7) is emerging as a ubiquitous route for lowering proliferative capacity as meta...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2007.07.004

    authors: Barkley LR,Hong HK,Kingsbury SR,James M,Stoeber K,Williams GH

    更新日期:2007-10-15 00:00:00

  • MiR-212-3p inhibits LPS-induced inflammatory response through targeting HMGB1 in murine macrophages.

    abstract::Sepsis is a major cause of mortality in seriously ill patients characterized by a series of severe systemic inflammatory responses due to an infection. Thus, there is a critically need to search more accurate biomarkers and targets for diagnosis and treatment of sepsis. Our study showed that miR-212-3p was up-regulate...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2016.12.008

    authors: Chen W,Ma X,Zhang P,Li Q,Liang X,Liu J

    更新日期:2017-01-15 00:00:00

  • Protein phosphatase 2A is involved in the regulation of protein kinase A signaling pathway during in vitro chondrogenesis.

    abstract::We have evaluated the importance of the Ser/Thr protein phosphorylation and dephosphorylation for chondrogenesis in high-density chicken limb bud mesenchymal cell cultures (HDCs) by using H89, a cell-permeable protein kinase inhibitor, and okadaic acid (OA), a phosphoprotein phosphatase (PP)-specific inhibitor molecul...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2002.5487

    authors: Zákány R,Szucs K,Bakó E,Felszeghy S,Czifra G,Bíró T,Módis L,Gergely P

    更新日期:2002-04-15 00:00:00

  • Dysregulation of Ack1 inhibits down-regulation of the EGF receptor.

    abstract::The protein tyrosine kinase Ack1 has been linked to cancer when over-expressed. Ack1 has also been suggested to function in clathrin-mediated endocytosis and in down-regulation of the epidermal growth factor (EGF) receptor (EGFR). We have studied the intracellular localization of over-expressed Ack1 and found that Ack...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2007.12.017

    authors: Grøvdal LM,Johannessen LE,Rødland MS,Madshus IH,Stang E

    更新日期:2008-04-01 00:00:00

  • Cytotoxic T cell responses to Streptococcus are associated with improved prognosis of oral squamous cell carcinoma.

    abstract::Several species of Streptococcus, such as S. salivarius, S. mitis, and S. anginosus, are found to extensively colonize the oral cavity and the upper respiratory tract, and have been shown to increase in patients with oral squamous cell carcinoma (OSCC). Accumulating evidence have revealed that commensal bacteria are i...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2017.11.018

    authors: Wang J,Sun F,Lin X,Li Z,Mao X,Jiang C

    更新日期:2018-01-01 00:00:00

  • TRIM39 is a MOAP-1-binding protein that stabilizes MOAP-1 through inhibition of its poly-ubiquitination process.

    abstract::Bax, a multi-domain pro-apoptotic Bcl-2 family member, is a key regulator for the release of apoptogenic factors from mitochondria. MOAP-1, which was first isolated from a screen for Bax-associating proteins, interacts with Bax upon apoptotic induction. MOAP-1 is a short-lived protein that is constitutively degraded b...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2008.11.021

    authors: Lee SS,Fu NY,Sukumaran SK,Wan KF,Wan Q,Yu VC

    更新日期:2009-04-15 00:00:00

  • Migrational changes of mesenchymal stem cells in response to cytokines, growth factors, hypoxia, and aging.

    abstract::Mesenchymal stem cells (MSCs) are non-immunogenic, multipotent cells with at least trilineage differentiation potential. They promote wound healing, improve regeneration of injured tissue, and mediate numerous other health effects. MSCs migrate to sites of injury and stimulate repair either through direct differentiat...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2015.08.019

    authors: Naaldijk Y,Johnson AA,Ishak S,Meisel HJ,Hohaus C,Stolzing A

    更新日期:2015-10-15 00:00:00

  • Residues in the alternative reading frame tumor suppressor that influence its stability and p53-independent activities.

    abstract::The Alternative Reading Frame (ARF) protein suppresses tumorigenesis through p53-dependent and p53-independent pathways. Most of ARF's anti-proliferative activity is conferred by sequences in its first exon. Previous work showed specific amino acid changes occurred in that region during primate evolution, so we progra...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2009.01.010

    authors: di Tommaso A,Hagen J,Tompkins V,Muniz V,Dudakovic A,Kitzis A,Ladeveze V,Quelle DE

    更新日期:2009-04-15 00:00:00

  • Shape of my heart: Cell-cell adhesion and cytoskeletal dynamics during Drosophila cardiac morphogenesis.

    abstract::The fruit fly Drosophila melanogaster has recently emerged as an excellent system to investigate the genetics of cardiovascular development and disease. Drosophila provides an inexpensive and genetically-tractable in vivo system with a large number of conserved features. In addition, the Drosophila embryo is transpare...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审

    doi:10.1016/j.yexcr.2017.03.057

    authors: McFaul CMJ,Fernandez-Gonzalez R

    更新日期:2017-09-01 00:00:00

  • RelA/NF-kappaB transcription factor associates with alpha-actinin-4.

    abstract::The NF-kappaB/RelA family of transcription factors regulates inducible transcription of a large number of genes in response to diverse stimuli. Little is known, however, about the location of NF-kappaB in the cytoplasm and the transport mechanism to the nucleus. We found that NF-kappaB is associated with the actin-bin...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2007.12.001

    authors: Babakov VN,Petukhova OA,Turoverova LV,Kropacheva IV,Tentler DG,Bolshakova AV,Podolskaya EP,Magnusson KE,Pinaev GP

    更新日期:2008-03-10 00:00:00

  • Poly(ADP ribose) polymerase-defective mutant cell clone of mouse L1210 cells.

    abstract::By a sequential mutation and selection utilizing N-methyl-N'-nitro-N-nitrosoguanidine as a mutagen, we succeeded in separating a poly(ADP ribose) polymerase-defective mutant clone (Cl-3527) from a mouse L1210 cell clone (Cl-3). The enzyme activity per cell in Cl-3527 cells was only 8% of that in wild type L1210 (CCL 2...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/s0014-4827(05)80080-1

    authors: Yoshihara K,Itaya A,Hironaka T,Sakuramoto S,Tanaka Y,Tsuyuki M,Inada Y,Kamiya T,Ohnishi K,Honma M

    更新日期:1992-05-01 00:00:00

  • Spontaneous apoptosis of thymocytes is uncoupled with progression through the cell cycle.

    abstract::Most developing lymphocytes spontaneously die in the thymus during positive and negative selection of the T cell repertoire. By evaluating the expression of the proliferation antigens Ki-67 and PCNA, we demonstrated here that more than 95% of thymocytes are potentially proliferating. The coincidence within the same ce...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1996.0382

    authors: Pellicciari C,Bottone MG,Schaack V,Barni S,Manfredi AA

    更新日期:1996-12-15 00:00:00

  • An investigation of adhesion and detachment in slime mould amoebae using columns of hydrophobic beads.

    abstract::Vegetative amoebae of Dictyostelium discoideum (strain AX2) adhere to columns of hydrophobic beads (Bio-Beads SM-2) which have been coated with axenic growth medium. This binding is blocked by sodium azide and other inhibitors of aerobic respiration. Bound cells are not detached by lowering the temperature but they ca...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(84)90235-0

    authors: Glynn PJ,Clarke KR

    更新日期:1984-05-01 00:00:00

  • Transforming growth factor beta stimulates fibroblast-collagen matrix contraction by different mechanisms in mechanically loaded and unloaded matrices.

    abstract::Studies were carried out to test the idea that transforming growth factor beta (TGFbeta) stimulated fibroblast contraction of collagen matrices by different mechanisms depending on mechanical loading on the cells and matrix. Under mechanically unloaded conditions (floating matrices), TGFbeta stimulated contraction dir...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2001.5445

    authors: Grinnell F,Ho CH

    更新日期:2002-02-15 00:00:00

  • Isolation of a fraction enriched in the trans-Golgi network from baby hamster kidney cells.

    abstract::Incubation of cultured cells at 20 degrees C blocks the transport of newly synthesized plasma membrane proteins, and the proteins accumulate intracellularly in a terminally glycosylated form. When baby hamster kidney cells are infected with the ts O45 mutant of vesicular stomatitis virus, and incubated at 20 degrees C...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(88)90190-5

    authors: de Curtis I,Howell KE,Simons K

    更新日期:1988-04-01 00:00:00

  • Investigation of the role of G1/S cell cycle mediators in cellular senescence.

    abstract::Cellular senescence is a state of irreversible cell cycle arrest in which normal cells at the end of their lifespan fail to enter into DNA synthesis upon serum or growth factor stimulation. We examined whether proteins required for G1/S cell cycle progression were irreversibly down-regulated in senescent human fibrobl...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1993.1306

    authors: Afshari CA,Vojta PJ,Annab LA,Futreal PA,Willard TB,Barrett JC

    更新日期:1993-12-01 00:00:00

  • Cytoskeleton protein 4.1R suppresses murine keratinocyte cell hyperproliferation via activating the Akt/ERK pathway in an EGFR-dependent manner.

    abstract::The correct functioning of epidermal growth factor receptor (EGFR), a receptor tyrosine kinase, is required for normal skin development and homeostasis. Cellular hyperproliferation induced by dysregulation of EGFR is tightly associated with structural and functional defects of hair follicles, skin lesions, and tumorig...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2019.111648

    authors: Chen L,Wang T,Ji X,Ding C,Liang T,Liu X,Lu J,Guo X,Kang Q,Ji Z

    更新日期:2019-11-15 00:00:00

  • Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro.

    abstract::The goal of this study was to assess the in vitro differentiation capacity of human bone marrow-derived stem cells (hBMSCs) along retinal lineages. Mononuclear cells (MNC) were isolated from bone marrow (BM) and mobilized peripheral blood (mPB) using Ficoll-Paque density gradient centrifugation, and were sorted by mag...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2015.02.001

    authors: Mathivanan I,Trepp C,Brunold C,Baerlocher G,Enzmann V

    更新日期:2015-04-10 00:00:00

  • Transforming growth factor-beta 1 has both promoting and inhibiting effects on induction of DNA synthesis in human fibroblasts.

    abstract::Growth-arrested human normal fibroblasts, TIG-1, initiated DNA synthesis following addition of epidermal growth factor (EGF). Transforming growth factor-beta 1 (TGF-beta 1) by itself had no effect on induction of DNA synthesis. When EGF and TGF-beta 1 were added simultaneously to growth-arrested TIG-1 cells, induction...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1995.1014

    authors: Fukami J,Tsuji K,Ueno A,Ide T

    更新日期:1995-01-01 00:00:00

  • cxcl12 gene engineered endothelial progenitor cells further improve the functions of oligodendrocyte precursor cells.

    abstract::Oligodendrocyte precursor cells (OPCs) are needed for white matter repair after various brain injury. Means that promote OPC functions could benefit white matter recovery after injury. Chemokine CXCL12 and endothelial progenitor cells (EPCs) both have been shown to promote remyelination. We hypothesize that the benefi...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2018.03.040

    authors: Yuan F,Chang S,Luo L,Li Y,Wang L,Song Y,Qu M,Zhang Z,Yang GY,Wang Y

    更新日期:2018-06-15 00:00:00

  • Calpain translocation during muscle fiber necrosis and regeneration in dystrophin-deficient mice.

    abstract::Previous studies have shown that calpains are autolytically cleaved during the disease process of mdx dystrophy, a mouse model for Duchenne muscular dystrophy, indicating that calpains may be activated and play a role in proteolysis that occurs in muscular dystrophy (J. Biol. Chem. 270(18), 10909-10914, 1995). In the ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1996.0227

    authors: Spencer MJ,Tidball JG

    更新日期:1996-08-01 00:00:00

  • Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining.

    abstract::On staining with a monoclonal antibody raised against microtubule-associated protein-1 (MAP-1), dot-like structures were seen in the nuclei of interphase cells, but not in those of non-cycling G0-arrested cells. Dots were also not seen in the nuclei of non-cycling senescent human cells (IMR-90). A SV40-DNA-transformed...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(86)90062-5

    authors: Ohno T,Kako R,Sato C,Ohkawa A

    更新日期:1986-04-01 00:00:00

  • Immunological identity of proteins that bind stored 5S RNA in Xenopus oocytes.

    abstract::In small oocytes of Xenopus laevis, the three most abundant proteins are isolated as basic polypeptides with molecular weights of 48 kD (P48), 43 kD (P43) and 40 kD (P40, also known as transcription factor IIIA). All three proteins share common properties in being able to bind specifically ribosomal 5S RNA molecules a...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(84)90602-5

    authors: Barrett P,Johnson RM,Sommerville J

    更新日期:1984-08-01 00:00:00