Elimination of mycoplasmas from cell cultures utilizing hyperimmune sera.

abstract：:The progression of cancer cells to highly malignant phenotypes that ultimately invade and metastasize to near and distant sites requires that metastatic cells respond to mitogenic signals in new microenvironments. The successful growth of metastases is due to their ability to respond to local paracrine growth factors ...

journal_title：Experimental cell research

authors： Nicolson GL

更新日期：1993-02-01 00:00:00

abstract：:The inhibition of apoptosis by Toxoplasma gondii is governed by its modulation of several signaling cascades including the NFkappaappaB and JNK pathways. This is evident in the dysregulation of JNK activation following treatment with UV and TNFalpha, both apoptogenic stimuli. Infection-mediated interference with the J...

journal_title：Experimental cell research

authors： Carmen JC,Southard RC,Sinai AP

更新日期：2008-12-10 00:00:00

abstract：:Dystrophin is a cytoskeletal protein normally expressed underneath the sarcolemma of muscle fibers. The lack of dystrophin in Duchenne muscular Dystrophy (DMD) muscles results in fiber necrosis, which was proposed to be mediated by chronic calcium mishandling. The extensive comparison of dystrophic cells from human or...

journal_title：Experimental cell research

authors： Marchand E,Constantin B,Balghi H,Claudepierre MC,Cantereau A,Magaud C,Mouzou A,Raymond G,Braun S,Cognard C

更新日期：2004-07-15 00:00:00

abstract：:Gamma delta (γδ) T cell-based tumor immunotherapy has been one of the most promising cancer immunotherapeutic strategies. However, the key regulators of the Vγ9Vδ2 T cell-mediated antitumor response remain unclear. Recently, mounting reports have indicated that Tim-3 performs critical roles in the regulation of the ac...

journal_title：Experimental cell research

authors： Li X,Lu H,Gu Y,Zhang X,Zhang G,Shi T,Chen W

更新日期：2020-01-01 00:00:00

abstract：:Adhesion to the extracellular matrix regulates numerous changes in the actin cytoskeleton by regulating the activity of the Rho family of small GTPases. Here, we report that adhesion and the associated changes in cell shape and cytoskeletal tension are all required for GTP-bound RhoA to activate its downstream effecto...

journal_title：Experimental cell research

authors： Bhadriraju K,Yang M,Alom Ruiz S,Pirone D,Tan J,Chen CS

更新日期：2007-10-01 00:00:00

abstract：:A monoclonal antibody Pr-28 was prepared, which recognized an antigen present only in proliferating cells. Immunofluorescence analysis of Pr-28 antigen showed that the antigen was localized mainly in perinuclear cytoplasm. Although Pr-28 antibody was produced against a chicken cell antigen, it reacts not only with chi...

journal_title：Experimental cell research

authors： Matsuhashi S,Watanabe T,Hori K

更新日期：1987-06-01 00:00:00

abstract：:The scavenger receptor (SR-A) is considered to play a role in host defense by scavenging endotoxins, oxidized proteins, and denatured or otherwise modified self components, which are routed toward degradation in macrophages. Recent data suggest that SR-A also functions as an adhesion molecule. Our previous finding of ...

journal_title：Experimental cell research

authors： Yokota T,Ehlin-Henriksson B,Hansson GK

更新日期：1998-02-25 00:00:00

abstract：:Total epidermal keratinocytes are a heterogeneous population of cells, including undifferentiated stem/progenitor cells (EpSPs) and their more differentiated progeny (Non-SP cells). Our previous in vivo data showed that EpSPs enhanced blood flow restoration when injected into an ischemic limb, whereas Non-SP cells had...

journal_title：Experimental cell research

authors： Oberley CC,Gourronc F,Hakimi S,Riordan M,Bronner S,Jiao C,Dunnwald M

更新日期：2008-02-15 00:00:00

abstract：:Endocytosis of signaling receptors, such as epidermal growth factor receptor (EGFR), tightly controls the signal transduction process triggered by ligand activation of these receptors. To identify new regulators of the endocytic trafficking of EGFR an RNA interference screen was performed for genes involved in ubiquit...

journal_title：Experimental cell research

authors： Duex JE,Mullins MR,Sorkin A

更新日期：2010-08-01 00:00:00

abstract：:Macrophage polarization plays a crucial role in regulating myocardial inflammation and injuries of coxsackievirus B3 (CVB3)-induced viral myocarditis (VM). It has been reported that miR-223 is a potent regulator of inflammatory responses that involved in macrophage polarization. However, the functional roles of miR-22...

journal_title：Experimental cell research

authors： Gou W,Zhang Z,Yang C,Li Y

更新日期：2018-05-01 00:00:00

abstract：:Accumulating evidence has suggested that thyroid hormone receptor interacting protein 6 (TRIP6) is a novel tumor-related regulator that is aberrantly expressed in multiple tumors and contributes to tumor progression and metastasis. Yet, little is known about the role of TRIP6 in cervical cancer. In the current study, ...

journal_title：Experimental cell research

authors： Yang F,Li L,Zhang J,Zhang J,Yang L

更新日期：2020-11-01 00:00:00

abstract：:A monoclonal antibody raised against microtubule-associated protein-1 (MAP-1) binds to nuclei of normal human fibroblasts, its binding site being detected as intranuclear immunofluorescent dots. The percentage of cells showing these dots increased with insulin, hydrocortisone, epidermal growth factor (EGF), and transf...

journal_title：Experimental cell research

authors： Ohno T,Ohkawa A,Sato C

更新日期：1985-06-01 00:00:00

abstract：:Mesenchymal stem cells (MSCs) secrete paracrine factors that could be cytoprotective and serve roles in immunoregulation during tissue injury. Although MSCs express HIV receptors, and co-receptors, and are susceptible to HIV infection, whether HIV-1 may affect biological properties of MSCs needs more study. We evaluat...

journal_title：Experimental cell research

authors： Cheng K,Rai P,Lan X,Plagov A,Malhotra A,Gupta S,Singhal PC

更新日期：2013-08-15 00:00:00

abstract：:MyD88, a protein implicated in interleukin-1 signaling, was localized in HeLa cells transiently transfected with an epitope-tagged (flag) version of MyD88. Overexpression of MyD88 can induce apoptosis. We have analyzed the fine structural intracellular distribution of MyD88 using immunoelectron microscopy. MyD88 is lo...

journal_title：Experimental cell research

authors： Jaunin F,Burns K,Tschopp J,Martin TE,Fakan S

更新日期：1998-08-25 00:00:00

abstract：:Approx. 35% of the DNA of cultured cells from the cactus mouse, Peromuscus eremicus, is contained in highly condensed constitutive heterochromatin which can be visualized in metaphase chromosomes stained by the C-band technique. Previous studies have shown this constitutive heterochromatin to contain a large proportio...

journal_title：Experimental cell research

authors： Halleck MS,Schlegel RA

更新日期：1983-09-01 00:00:00

abstract：:The technique of fiber DNA measurement was used to study the possibility that the lengthening of the DNA "S" phase previously reported for mouse premeiotic spermatogonia was due to a reduced number of initiation sites. The mean replicon size of neonatal mouse preleptotene cells was similar to sizes reported for adult ...

journal_title：Experimental cell research

authors： Jagiello G,Sung WK,Van't Hof J

更新日期：1983-07-01 00:00:00

abstract：:Current methods of monitoring insulin in culture are limited to soluble insulin (secretions or lysates) or synthetic gene reporter analyses. We present an insulin-specific cell enzyme-linked immunosorbent assay (cell-ELISA) to assess relative intracellular insulin protein content of adherent cultures, normalized to ce...

journal_title：Experimental cell research

authors： Andersen PL,Vermette P

更新日期：2016-09-10 00:00:00

abstract：:The long non-coding RNAs (lncRNAs) have gained much attention due to its essential roles in molecular regulation. As one of the classic lncRNAs, H19 is strongly expressed during embryogenesis and plays a crucial biological function during development. Mesenchymal stem cells (MSCs) are an ideal cell source for tissue e...

journal_title：Experimental cell research

authors： Liu Y,Li G,Zhang JF

更新日期：2017-11-15 00:00:00

abstract：:Human cyclin D1 forms complexes with several Cdks, with proliferating cell nuclear antigen, and with a recently discovered 21-kDa inhibitor of Cdk activity. Substrates for cyclin D1/Cdks have not been identified in vivo, but it has been proposed that the D class of cyclins might play a role in regulating the activity ...

journal_title：Experimental cell research

authors： Atadja P,Wong H,Veillete C,Riabowol K

更新日期：1995-04-01 00:00:00

abstract：:JANEX-1/WHI-P131, a selective Janus kinase 3 (JAK3) inhibitor, has been shown to delay the onset of diabetes in the NOD mouse model. However, the molecular mechanism by which JANEX-1 protects pancreatic beta-cells is unknown. In the current study, we investigated the role of JANEX-1 on interleukin (IL)-1beta and inter...

journal_title：Experimental cell research

authors： Lv N,Kim EK,Song MY,Choi HN,Moon WS,Park SJ,Park JW,Kwon KB,Park BH

更新日期：2009-07-15 00:00:00

abstract：:During skeletal muscle development three intermediate filament proteins are expressed: nestin, vimentin, and desmin. Vimentin and desmin belong to the class III intermediate filaments and are closely related to each other, whereas nestin is a more distantly related, class VI, intermediate filament. It was previously o...

journal_title：Experimental cell research

authors： Sjöberg G,Jiang WQ,Ringertz NR,Lendahl U,Sejersen T

更新日期：1994-10-01 00:00:00

abstract：:During mitosis there is an abrupt inhibition of a wide range of membrane functions, including fluid-phase and adsorptive pinocytosis. We have used cell hybrids formed between mitotic and interphase cells to approach the mechanism of this inhibition. We report that fluid pinocytosis is reactivated in the mitotic partne...

journal_title：Experimental cell research

authors： Oliver JM,Seagrave JC

更新日期：1987-08-01 00:00:00

abstract：:In low serum (0.2%) medium, ascorbate stimulates primary avian tendon cells to increase procollagen synthesis from 12 to 50% of total protein synthesis. This is reversibly blocked by an increase of serum levels from 0.2 to 3%. Ascorbate in low serum medium has been shown previously to stimulate the procollagen pathway...

journal_title：Experimental cell research

authors： Valmassoi J,Schwarz RI

更新日期：1988-06-01 00:00:00

abstract：:N-methyl-d-aspartate (NMDA) subtype of glutamate receptors plays an important role during brain development and receptor activation has been shown to promote neuronal survival. The mechanisms by which NMDA increases neuronal survival are not fully understood. Here, we show that treatment with low concentrations of NMD...

journal_title：Experimental cell research

authors： Korhonen L,Näpänkangas U,Steen H,Chen Y,Martinez R,Lindholm D

更新日期：2004-05-01 00:00:00

abstract：:The turbidity of axonemes during active sliding of microtubules was analysed using the stopped-flow-light-scattering method with high time resolution. Flagella of sea-urchin spermatozoa were demembranated and used after a brief treatment with trypsin. The turbidity of the suspension of flagellar axonemes during ATP-in...

journal_title：Experimental cell research

authors： Kamimura S,Nakanishi M,Yano M,Shimizu H

更新日期：1986-03-01 00:00:00

abstract：:Contraction of 3D collagen matrices by fibroblasts frequently is used as an in vitro model of wound closure. Different iterations of the model - all conventionally referred to as "contraction" - involve different morphological patterns. During floating matrix contraction, cells initially are round without stress fiber...

journal_title：Experimental cell research

authors： Liu Z,Ho CH,Grinnell F

更新日期：2014-08-15 00:00:00

abstract：:Extracellular matrix (ECM) has been reported to enhance epithelial cell attachment and proliferation as well as to induce differentiation in vitro. In an attempt to determine the benefits of culturing pancreatic islet cells on ECM, we studied the morphological and functional patterns of rat islet cells and an insulin-...

journal_title：Experimental cell research

authors： Thivolet CH,Chatelain P,Nicoloso H,Durand A,Bertrand J

更新日期：1985-08-01 00:00:00

abstract：:Keratinocytes in human skin undergo apoptosis during various inflammatory processes and after ultraviolet (UV) irradiation. To determine if keratinocyte apoptosis may be mediated by the Fas/APO-1 receptor (CD95), a signal transduction pathway known to initiate programmed cell death of lymphocytes, we investigated Fas ...

journal_title：Experimental cell research

authors： Leverkus M,Yaar M,Gilchrest BA

更新日期：1997-05-01 00:00:00

abstract：:Despite great progress for two decades in microRNAs (miRNAs), the direct regulation of host gene by intragenic (mostly intronic) miRNA is conceptually plausible but evidence-limited. Here, we report that intronic miR-932 could target its host gene via binding with coding sequence (CDS) region rather than regular 3'UTR...

journal_title：Experimental cell research

authors： Qian J,Tu R,Yuan L,Xie W

更新日期：2016-06-10 00:00:00

abstract：:In culture, nontransformed human diploid fibroblasts divide a limited number of times, resulting in a nonproliferating senescent cell culture which exhibits an altered pattern of gene expression. Previously we reported that an early event in the process of replicative senescence was an increase in the synthesis of two...

journal_title：Experimental cell research

authors： Zeng G,McCue HM,Mastrangelo L,Millis AJ

更新日期：1996-11-01 00:00:00