Abstract：
:Transepithelial resistance (TER) measurement has often been used to study the paracellular transport properties of epithelia grown on permeable filters, especially the barrier function of tight junctions. However, the TER value includes another source, the resistance caused by cell-substrate contact, that may give rise to a high TER value if cell-substrate separation is small. In this study we use electric cell-substrate impedance sensing (ECIS) to measure both paracellular resistance and the average cell-substrate distance of MDCK (II), HEp-2, and WI-38 VA13 cells. Comparing ECIS data with those from TER measurements of cell layers cultured on polycarbonate filters, we can obtain the approximate extra resistance resulting from cell-substrate contact for each cell type. The value of cell-substrate resistance was also estimated by two theoretical calculations that bracket the true values. Our results demonstrate that cell-substrate contact substantially influences the TER data measured using polycarbonate filters and that the extra resistance due to cell-substrate spaces depends on both cell type and filter property.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Lo CM,Keese CR,Giaever Idoi
10.1006/excr.1999.4538keywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
576-80issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(99)94538-Xjournal_volume
250pub_type
杂志文章abstract：:In primary cultures of ovine thyroid cells, TSH induced the expression of several differentiated functions including the formation of follicles, and synthesis and storage of iodinated thyroglobulin in the follicular lumen. In the present report, these follicles were shown by transmission (TEM) and scanning electron mi...
journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3571
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：1991-03-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2018.02.022
更新日期：2018-04-15 00:00:00
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doi：10.1016/j.yexcr.2020.112402
更新日期：2021-01-01 00:00:00
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更新日期：2018-01-15 00:00:00
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doi：10.1016/j.yexcr.2018.05.019
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2003.11.009
更新日期：2004-03-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1210
更新日期：1993-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1006/excr.1998.4071
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doi：10.1006/excr.1995.1255
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2007.04.034
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1224
更新日期：1994-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2020-04-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.04.035
更新日期：2007-08-01 00:00:00
