Abstract：
:The effects of hormonal promotion of T24-ras oncogene-transfected rat embryo fibroblasts (REF) were compared to cotransformation of these cells with adenovirus E1A and ras. Cotransfection of E1A + ras resulted in the appearance of morphologically transformed cells which were very efficiently established into cell lines. Addition of glucocorticoid hormones to T24-ras-transfected REF cells resulted in cells with a transformed morphology and a capacity to form foci. These foci were, however, inefficiently established into stable cell lines. Removal of hormone from growing cells resulted in retarded growth, suggesting that the hormone acted as a growth factor on these cells. Both E1A-transformed cells and hormone-treated ras-transformed cells showed a reduction in synthesis of high molecular weight tropomyosin isoforms and a decreased expression of surface fibronectin. Control experiments demonstrated that the effects of hormone were mediated through the glucocorticoid receptor. Our findings suggest that glucocorticoid hormones may promote the in vitro growth of ras-initiated REF cells into stably transformed cell lines, but that this ability is limited compared to that of adenovirus E1A.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Marshall H,Martens I,Svensson C,Akusjärvi G,Linder Sdoi
10.1016/0014-4827(91)90126-fsubject
Has Abstractpub_date
1991-05-01 00:00:00pages
35-41issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(91)90126-Fjournal_volume
194pub_type
杂志文章abstract：:The inhibition of apoptosis by Toxoplasma gondii is governed by its modulation of several signaling cascades including the NFkappaappaB and JNK pathways. This is evident in the dysregulation of JNK activation following treatment with UV and TNFalpha, both apoptogenic stimuli. Infection-mediated interference with the J...
journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1988-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1989-06-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2003.12.021
更新日期：2004-04-15 00:00:00
abstract：:Fluorescence hybridization is a widely used technique in cell biology and pathology for detecting specific nucleic acid (DNA and RNA) sequences in fixed cells. This technique does not, however, provide dynamic information on the intracellular behavior of the targeted molecules. The aim of this work was to investigate ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.3464
更新日期：1997-02-25 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1220
更新日期：1993-09-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(82)90420-7
更新日期：1982-11-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90034-8
更新日期：1990-11-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90354-9
更新日期：1984-03-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2012-05-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90554-8
更新日期：1991-03-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1090
更新日期：1993-04-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1016/0014-4827(86)90531-8
更新日期：1986-07-01 00:00:00
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更新日期：2006-08-01 00:00:00
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journal_title：Experimental cell research
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更新日期：1984-04-01 00:00:00
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journal_title：Experimental cell research
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更新日期：1986-06-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2019-05-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2014-09-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1391
更新日期：1995-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.08.022
更新日期：2005-11-15 00:00:00
abstract：:The linker histone subtype H1.1 belongs to the group of main-type histones and is synthesized in somatic tissues as well as in germ cells during the S phase of the cell cycle. In adult mice the histone gene H1.1 is expressed mainly in thymus, spleen, and testis. The single-copy gene coding for the H1.1 protein was eli...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4767
更新日期：2000-02-25 00:00:00
abstract：:The precise distribution of DNA within the resting human T lymphocyte nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. The nucleolus is partially enveloped by a layer of condensed chromatin which, at several places, penetrates into the nucleola...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1110
更新日期：1993-04-01 00:00:00
