Isolation of an MSP1-derived transcriptionally active nucleolar particle.

abstract：:Hyperglycemia is a primary factor that disturbs podocyte function in the glomerular filtration process; this disturbance leads to the development of diabetic nephropathy, and ultimately, renal failure. Podocyte function may also be altered by biological agents that modify protein kinase activity, including the cGMP-ac...

journal_title：Experimental cell research

authors： Piwkowska A,Rogacka D,Audzeyenka I,Angielski S,Jankowski M

更新日期：2014-01-01 00:00:00

abstract：:Utilizing both the TET-OFF and TET-ON systems in combination with transcriptional control elements of the Tie-2 gene, we have established a series of transgenic activator and responder mice for TET-regulated endothelial cell-specific transgene expression in double transgenic mouse embryos and in adult mice. TET-regula...

journal_title：Experimental cell research

authors： Deutsch U,Schlaeger TM,Dehouck B,Döring A,Tauber S,Risau W,Engelhardt B

更新日期：2008-04-01 00:00:00

abstract：:In a recent screening for genes down regulated in replicatively senescent human umbilical vein endothelial cells (HUVECs), we have isolated the novel protein SNEV. Since then SNEV has proven as a multifaceted protein playing a role in pre-mRNA splicing, DNA repair, and the ubiquitin/proteosome system. Here, we report ...

journal_title：Experimental cell research

authors： Voglauer R,Chang MW,Dampier B,Wieser M,Baumann K,Sterovsky T,Schreiber M,Katinger H,Grillari J

更新日期：2006-04-01 00:00:00

abstract：:The life cycle of many cell types can hinge on the presence of death factors that can control programmed cell death. The Fas-mediated apoptotic pathway has been implicated in controlling apoptosis during spermatogenesis in a number of mammalian species. In the human, the presence of nuclear DNA damage in ejaculated sp...

journal_title：Experimental cell research

authors： Sakkas D,Mariethoz E,St John JC

更新日期：1999-09-15 00:00:00

abstract：:Previous morphological and biochemical studies have suggested that actin and actin-containing filaments (microfilaments) exist in the eukaryotic nucleus and that they perform important nuclear functions. However, the concept is not widely accepted. In this study, we demonstrate actin and bundles of actin in the nuclei...

journal_title：Experimental cell research

authors： Parfenov VN,Davis DS,Pochukalina GN,Sample CE,Bugaeva EA,Murti KG

更新日期：1995-04-01 00:00:00

abstract：:A monoclonal antibody, G8, which recognizes a form of tubulin (G8-tubulin) with a novel distribution in Rat-1 cells and Potorous tridactylis kidney (Ptk-2) cells was isolated. G8 labeled the interphase cytoskeleton of Rat-1 fibroblasts but not mitotic spindles or midbodies. G8 also stained a fiber network in some but ...

journal_title：Experimental cell research

authors： Gossels JM,Ingram VM

更新日期：1989-10-01 00:00:00

abstract：:Pulmonary arterial hypertension (PAH) is a progressive pulmonary vascular disease which is associated with pulmonary arterial endothelial cells (PAEC) dysfunction and pulmonary arterial smooth muscle cells proliferation. Moreover, inflammation is contributing a critical role in EC dysfunction and remains elusive. Nucl...

journal_title：Experimental cell research

authors： Li L,Kim IK,Chiasson V,Chatterjee P,Gupta S

更新日期：2017-10-01 00:00:00

abstract：:3-phosphoinositide-dependent kinase-1 (PDK1) phosphorylates and activates several kinases in the cAMP-dependent, cGMP-dependent and protein kinase C (AGC) family. Many putative PDK1 substrates have been identified, but have not been analyzed following transient and specific inhibition of PDK1 activity. Here, we demons...

journal_title：Experimental cell research

authors： Tamgüney T,Zhang C,Fiedler D,Shokat K,Stokoe D

更新日期：2008-07-01 00:00:00

abstract：:Centromeres are the chromosomal loci that direct the formation of the kinetochores. These macromolecular assemblies mediate the interaction between chromosomes and spindle microtubules and thereby power chromosome movement during cell division. They are also the sites of extensive regulation of the chromosome segregat...

journal_title：Experimental cell research

authors： Bernad R,Sánchez P,Losada A

更新日期：2009-11-15 00:00:00

abstract：:Our previous studies have showed that tumor-derived autophagosomes (termed "DRibbles") induce B cell activation, resulting in antibody production and cytokine secretion. Unexpectedly, we found that unfractionated splenocytes produced a higher level of antibody and cytokine than that of purified B cells. In the current...

journal_title：Experimental cell research

authors： Zhou M,Li W,Wen Z,Sheng Y,Ren H,Dong H,Cao M,Hu HM,Wang LX

更新日期：2015-02-15 00:00:00

abstract：:The Golgi apparatus plays roles in cell polarity, directional cell migration, and bipolar spindle assembly, as well as the secretary pathway. In addition, recent studies have suggested the Golgi-dependent control of mitotic entry. We studied the role of the centrosomal kinase Aurora A in maintaining the Golgi apparatu...

journal_title：Experimental cell research

authors： Kimura M,Takagi S,Nakashima S

更新日期：2018-06-01 00:00:00

abstract：:A two-site radio-immunoassay for beta NGF demonstrated 5-10 pg of NGF in the normal, adult rat iris. Ciliarectomy or sympathectomy did not significantly alter the amount of NGF after 10 days. However, denervation including all sensory axons (stereotactic lesion distal to the trigeminal ganglion) increased the level to...

journal_title：Experimental cell research

authors： Ebendal T,Olson L,Seiger A

更新日期：1983-10-15 00:00:00

abstract：:The scavenger receptor (SR-A) is considered to play a role in host defense by scavenging endotoxins, oxidized proteins, and denatured or otherwise modified self components, which are routed toward degradation in macrophages. Recent data suggest that SR-A also functions as an adhesion molecule. Our previous finding of ...

journal_title：Experimental cell research

authors： Yokota T,Ehlin-Henriksson B,Hansson GK

更新日期：1998-02-25 00:00:00

abstract：:Gallbladder cancer (GBC) is an aggressive malignancy of the bile duct, which is associated with a low (5-year) survival and poor prognosis. The transcription factor HIF-1α is implicated in the angiogenesis, cell survival, epithelial mesenchymal transition (EMT) and invasiveness of GBC. In this study, we have investiga...

journal_title：Experimental cell research

authors： Gao H,Xie J,Peng J,Han Y,Jiang Q,Han M,Wang C

更新日期：2015-03-15 00:00:00

abstract：:Heat shock proteins (hsp) have important roles in the regulation and protection of both prokaryotic and eukaryotic cells, especially during environmental stress. Hsps are also important bacterial virulence factors. We investigated whether bacterial hsp60 can alter epithelial cell mitogen-activated protein kinase (MAPK...

journal_title：Experimental cell research

authors： Zhang L,Pelech SL,Mayrand D,Grenier D,Heino J,Uitto VJ

更新日期：2001-05-15 00:00:00

abstract：:In this study we have analyzed short- and long-term changes in extracellular signal-regulated kinase (ERK) 1 and 2 activity during 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced differentiation of human promyelocytic leukemia cells. Immunoprecipitation of HL-60 cellular extracts with an ERK antibody followed by in...

journal_title：Experimental cell research

authors： Meighan-Mantha RL,Wellstein A,Riegel AT

更新日期：1997-08-01 00:00:00

abstract：:An attempt was made to determine the role of metabolic acidification of pericellular medium in regulating cell proliferation. A method of measuring the pH of pericellular medium at a distance exceeding a Debye radius of 5-10 A from the cell surface (pHp) was developed. The values of pHp and pH measured in the medium a...

journal_title：Experimental cell research

authors： Akatov VS,Lezhnev EI,Vexler AM,Kublik LN

更新日期：1985-10-01 00:00:00

abstract：:Rat thyroid differentiated cells (PC Cl 3) are an excellent model system with which to study the interaction between differentiation and cell transformation. We previously demonstrated that PC Cl 3 cells expressing the adenovirus E1A gene no longer depend on thyrotropin for growth and do not express thyroid differenti...

journal_title：Experimental cell research

authors： Iuliano R,Trapasso F,Stella A,Le Pera I,Melillo RM,Bruni P,Baldassarre G,Chiariotti L,Santoro M,Viglietto G,Fusco A

更新日期：2000-11-01 00:00:00

abstract：:The Drosophila sponge (spg)/CG31048 gene belongs to the dedicator of cytokinesis (DOCK) family genes that are conserved in a wide variety of species. DOCK family members are known as DOCK1-DOCK11 in mammals. Although DOCK1 and DOCK2 involve neurite elongation and immunocyte differentiation, respectively, the functions...

journal_title：Experimental cell research

authors： Eguchi K,Yoshioka Y,Yoshida H,Morishita K,Miyata S,Hiai H,Yamaguchi M

更新日期：2013-08-15 00:00:00

abstract：:Serelaxin, a recombinant form of human relaxin-2, is currently regarded as a novel drug for treatment of acute heart failure. However, whether therapeutic effects of serelaxin are achieved by inhibiting cardiac fibrosis remains unclear. In this study, we investigate effects of serelaxin on inhibiting cardiac fibrosis....

journal_title：Experimental cell research

authors： Wu XP,Wang HJ,Wang YL,Shen HR,Tan YZ

更新日期：2018-01-01 00:00:00

abstract：:Normal and transformed human cells when stained for ezrin, an F-actin-binding ERM (ezrin/radixin/moesin) family protein, revealed a faint and intense immunofluorescence, respectively. Surprisingly, nuclear staining that was assigned to the nucleolus by confocal laser and immunoelectron microscopy was detected in both ...

journal_title：Experimental cell research

authors： Kaul SC,Kawai R,Nomura H,Mitsui Y,Reddel RR,Wadhwa R

更新日期：1999-07-10 00:00:00

abstract：:Regulation of TSH receptor (TSHr) mRNA accumulation has been investigated in canine thyrocytes in primary culture by in situ hybridization experiments; the effects of the mitogenic thyrotropin (TSH), epidermal growth factor (EGF), and phorbol ester TPA (12-O-tetradecanoylphorbol-13-acetate) have been compared. Apart f...

journal_title：Experimental cell research

authors： Pohl V,Maenhaut C,Gérard C,Vassart G,Dumont JE

更新日期：1992-04-01 00:00:00

abstract：:Synthetic tubugis are equally potent but more stable than their natural forms. Their anticancer potential was estimated on a solid melanoma in vitro and in vivo. Tubugi-1 induced the apoptosis in B16 cells accompanied with strong intracellular production of reactive species, subsequently imposing glutathione and thiol...

journal_title：Experimental cell research

authors： Drača D,Mijatović S,Krajnović T,Pristov JB,Đukić T,Kaluđerović GN,Wessjohann LA,Maksimović-Ivanić D

更新日期：2019-07-15 00:00:00

abstract：:A monoclonal rat IgM antibody (4C9) raised against F9 embryonal carcinoma cells reacted with fucosyl residues in poly-N-acetyllactosamine-type large carbohydrates of these cells (embryoglycan). The chemical properties and distribution of the antigen resembled those of SSEA-1. The monoclonal antibody was found to inhib...

journal_title：Experimental cell research

authors： Nomoto S,Muramatsu H,Ozawa M,Suganuma T,Tashiro M,Muramatsu T

更新日期：1986-05-01 00:00:00

abstract：:The continuously decreasing willingness for liver donation aggravates treatment of end-stage liver diseases requiring organ transplantation as the only curative strategy. Cell therapy approaches using human hepatocytes or stem cell-derived hepatocyte-like cells may be a therapeutic option out of this dilemma. ABCB5-po...

journal_title：Experimental cell research

authors： Tietze L,Winkler S,Hempel M,Kluth MA,Tappenbeck N,Ganss C,Dooley S,Christ B

更新日期：2018-08-15 00:00:00

abstract：:In a previous study we have shown that triiodothyronine (T3) added to a serum-free medium supplemented with insulin, transferrin, and selenous acid (ITS) can stimulate Caco-2 cell differentiation. In this study we have focused on the effects of T3 on sucrase activity. The results obtained demonstrate that T3 (50 nM) d...

journal_title：Experimental cell research

authors： Jumarie C,Herring-Gillam FE,Beaulieu JF,Malo C

更新日期：1996-02-01 00:00:00

abstract：:The neurohormone serotonin (5HT) triggers meiosis reinitiation in oocytes of the pelecypod molluscs Spisula solidissima and Ruditapes philippinarum. However, while Spisula oocytes complete maturation, Ruditapes oocytes arrest in metaphase I. Anti-phosphotyrosine antibody revealed that serotonin triggered an early tyro...

journal_title：Experimental cell research

authors： Abdelmajid H,Rivailler P,Krantic S,Guerrier P

更新日期：1994-06-01 00:00:00

abstract：:Microfilament-associated proteins and membrane-microfilament interactions are being investigated in microvilli isolated from 13,762 rat mammary ascites tumor cells. "Phalloidin shift" analyses on velocity sedimentation gradients of Triton X-100 extracts of [3H]-glucosamine-labeled microvilli identified a 120-kDa cell-...

journal_title：Experimental cell research

authors： Vanderpuye OA,Carraway CA,Carraway KL

更新日期：1988-10-01 00:00:00

abstract：:The measurement of chromomycin A3/DAPI fluorescence ratios is shown to allow base content determinations in eu- and heterochromatic regions of interphase cell nuclei. The base content values obtained in chromocenters and euchromatin of Scilla sibirica agree with those measured earlier [12] on the band and non-band are...

journal_title：Experimental cell research

authors： Leemann U,Ruch F

更新日期：1983-09-01 00:00:00

abstract：:β-catenin is a key mediator of Wnt signaling and its deregulated nuclear accumulation can drive cancer progression. While the central armadillo (Arm) repeats of β-catenin stimulate nuclear entry, the N- and C-terminal "tail" sequences are thought to regulate turnover and transactivation. We show here that the N- and C...

journal_title：Experimental cell research

authors： Sharma M,Jamieson C,Lui C,Henderson BR

更新日期：2016-11-01 00:00:00