Abstract:
:Rat thyroid differentiated cells (PC Cl 3) are an excellent model system with which to study the interaction between differentiation and cell transformation. We previously demonstrated that PC Cl 3 cells expressing the adenovirus E1A gene no longer depend on thyrotropin for growth and do not express thyroid differentiation markers. Here we show that an E1A mutant unable to bind the RB protein failed to transform the PC Cl 3 cells. Conversely, mutations in the E1A p300 interacting region did not affect its transforming ability. The pivotal role of RB family proteins in the thyroid cell transformation is supported by the thyrotropin independence induced by the E7 gene of human papilloma virus type 16, but not by a mutated form in the RB-binding region.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Iuliano R,Trapasso F,Stella A,Le Pera I,Melillo RM,Bruni P,Baldassarre G,Chiariotti L,Santoro M,Viglietto G,Fusco Adoi
10.1006/excr.2000.5023keywords:
subject
Has Abstractpub_date
2000-11-01 00:00:00pages
257-67issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(00)95023-7journal_volume
260pub_type
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