Pivotal role of the RB family proteins in in vitro thyroid cell transformation.

Abstract:

:Rat thyroid differentiated cells (PC Cl 3) are an excellent model system with which to study the interaction between differentiation and cell transformation. We previously demonstrated that PC Cl 3 cells expressing the adenovirus E1A gene no longer depend on thyrotropin for growth and do not express thyroid differentiation markers. Here we show that an E1A mutant unable to bind the RB protein failed to transform the PC Cl 3 cells. Conversely, mutations in the E1A p300 interacting region did not affect its transforming ability. The pivotal role of RB family proteins in the thyroid cell transformation is supported by the thyrotropin independence induced by the E7 gene of human papilloma virus type 16, but not by a mutated form in the RB-binding region.

journal_name

Exp Cell Res

authors

Iuliano R,Trapasso F,Stella A,Le Pera I,Melillo RM,Bruni P,Baldassarre G,Chiariotti L,Santoro M,Viglietto G,Fusco A

doi

10.1006/excr.2000.5023

keywords:

subject

Has Abstract

pub_date

2000-11-01 00:00:00

pages

257-67

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(00)95023-7

journal_volume

260

pub_type

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