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Assessment of the hepatocytic differentiation ability of human skin-derived ABCB5+ stem cells.

Abstract：

:The continuously decreasing willingness for liver donation aggravates treatment of end-stage liver diseases requiring organ transplantation as the only curative strategy. Cell therapy approaches using human hepatocytes or stem cell-derived hepatocyte-like cells may be a therapeutic option out of this dilemma. ABCB5-positive mesenchymal stromal cells from human skin featured promising potential to treat immune-mediated diseases. Since most of chronic liver diseases involve exaggerating immune mechanisms, it was the aim to demonstrate in this study, whether ABCB5+ stem cells may serve as a resource to generate hepatocytic cells for application in liver cell transplantation. Using an established single-step protocol, which had been successfully applied to differentiate mesenchymal stromal cells into the hepatocytic lineage, ABCB5+ skin-derived stem cells did not gain significant characteristics of hepatocytes. Yet, upon culture in hepatocytic differentiation medium, ABCB5+ stem cells secreted immunomodulatory and anti-fibrotic factors as well as proteins, which may prompt hepatic morphogenesis besides others. Hepatic transplantation of ABCB5+ stem cells, which had been prior cultured in hepatocytic differentiation medium, did not cause any obvious deterioration of liver architecture suggesting their safe application. Thus, human ABCB5+ skin-derived stem cells secreted putative hepatotropic factors after culture in hepatocytic differentiation medium.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Tietze L,Winkler S,Hempel M,Kluth MA,Tappenbeck N,Ganss C,Dooley S,Christ B

doi

10.1016/j.yexcr.2018.05.040

subject

Has Abstract

pub_date

2018-08-15 00:00:00

pages

335-347

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(18)30322-7

journal_volume

369

pub_type

杂志文章

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