Determination of downstream targets of FGF signalling using gene trap and cDNA subtractive approaches.


:Signalling through the fibroblast growth factor family (FGF) of ligands is essential for normal mammalian embryonic development. At a cellular level, many details of the molecular basis of the signal transduction process have been uncovered, but our knowledge of the identity of the downstream effectors of the FGF signal in the developing embryo remains limited. We have used two independent approaches to begin to identify downstream targets of FGF signalling in the embryo: (1). a gene trap approach and (2). cDNA subtraction, using mouse embryonic stem (ES) cells as a cellular system representative of an early window on the developing embryo. Both approaches led to the identification of a number of targets of FGF signalling, and we provide data to show that the chaperone Mrj, the tumour antigen Tum, collapsin mediator response protein Crmp, a novel transcriptional repressor Nac1 and ribophorin are all differentially regulated following FGF signalling. Independent gene trapping of Mrj previously indicated a role for the gene in embryogenesis [Development 126 (1999) 1247], and we present transcript data implicating a number of the newly isolated FGF target genes in different embryonic processes.


Exp Cell Res


Tateossian H,Powles N,Dickinson R,Ficker M,Maconochie M





Has Abstract


2004-01-01 00:00:00














  • Interleukin-2 induces a rapid increase in ornithine decarboxylase mRNA in a cloned murine T lymphocytic cell line.

    abstract::We recently observed a 25-fold increase in the activity of ornithine decarboxylase (ODC) 6 h after treatment of G1-synchronized CTLL-2 cells with interleukin-2 (IL-2). Here we show that the ODC mRNA content increased in parallel with the ODC activity during the first hours of stimulation with IL-2, resulting in a 25-f...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Legraverend C,Potter A,Hölttä E,Alitalo K,Andersson LC

    更新日期:1989-03-01 00:00:00

  • miR-125b/Ets1 axis regulates transdifferentiation and calcification of vascular smooth muscle cells in a high-phosphate environment.

    abstract:OBJECTIVES:Vascular calcification is highly prevalent in patients with chronic kidney disease (CKD) and contributes to increased risk of cardiovascular disease and mortality. Accumulated evidences suggested that vascular smooth muscle cells (VSMCs) to osteoblast-like cells transdifferentiation (VOT) plays a crucial rol...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wen P,Cao H,Fang L,Ye H,Zhou Y,Jiang L,Su W,Xu H,He W,Dai C,Yang J

    更新日期:2014-04-01 00:00:00

  • Vascular smooth muscle cell detachment from elastin and migration through elastic laminae is promoted by chondroitin sulfate-induced "shedding" of the 67-kDa cell surface elastin binding protein.

    abstract::Impaired elastin fiber assembly is observed in the fetal ductus arteriosus (DA), associated with a reduced concentration of elastin binding protein (EBP), a 67-kDa galactolectin. It is also seen in cultured aortic (Ao) smooth muscle cells (SMC) following the release of the EBP by glycosaminoglycans rich in N-acetylgal...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hinek A,Boyle J,Rabinovitch M

    更新日期:1992-12-01 00:00:00

  • Cyclosporin A is a differential inhibitor of eukaryotic RNA polymerases.

    abstract::In order to investigate the molecular mechanism of biological action of the drug cyclosporin A (CsA), we have studied its effect on eukaryotic RNA polymerase activities in two systems: (a) in vitro transcription; (b) injection into frog oocytes. The electron microscopic transcription R-loop method [1] has been used to...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Brack C,Mattaj IW,Gautschi J,Cammisuli S

    更新日期:1984-04-01 00:00:00

  • C-banding of Peromyscus constitutive heterochromatin persists following histone hyperacetylation.

    abstract::Approx. 35% of the DNA of cultured cells from the cactus mouse, Peromuscus eremicus, is contained in highly condensed constitutive heterochromatin which can be visualized in metaphase chromosomes stained by the C-band technique. Previous studies have shown this constitutive heterochromatin to contain a large proportio...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Halleck MS,Schlegel RA

    更新日期:1983-09-01 00:00:00

  • MicroRNA-182 inhibits HCMV replication through activation of type I IFN response by targeting FOXO3 in neural cells.

    abstract::Human cytomegalovirus (HCMV) has led to kinds of clinical disorders and great morbidity worldwide, such as sensorineural hearing loss (SNHL), mental retardation, and developmental delays in immunocompromised individuals. Congenital HCMV infection is a leading cause of birth defects, primarily manifesting as neurologic...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: He X,Teng J,Cui C,Li D,Wen L

    更新日期:2018-08-15 00:00:00

  • Identification of cell binding sites in the laminin alpha5-chain G domain.

    abstract::The laminins consist of at least 11 polypeptides (5 alpha-chains, 3 beta-chains, and 3 gamma-chains) specific to basement membranes. Here we investigate the biological activity associated with the G domain of the newly identified laminin alpha5-chain using 113 overlapping synthetic peptides (positions 2679-3635). Usin...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Makino M,Okazaki I,Kasai S,Nishi N,Bougaeva M,Weeks BS,Otaka A,Nielsen PK,Yamada Y,Nomizu M

    更新日期:2002-07-01 00:00:00

  • Effects of antiandrogens on chromatin remodeling and transcription of the integrated mouse mammary tumor virus promoter.

    abstract::Inhibition of the ligand-activated androgen receptor (AR) by antiandrogens plays an important role in the treatment of various hyperandrogenic disorders including prostate cancer. However, the molecular mechanisms of antiandrogen activity in vivo remain unclear. In this study we analyzed the effects of cyproterone ace...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: List HJ,Smith CL,Martinez E,Harris VK,Danielsen M,Riegel AT

    更新日期:2000-10-10 00:00:00

  • Differential regulation of X-chromosome-linked inhibitor of apoptosis protein (XIAP) and caspase-3 by NMDA in developing hippocampal neurons; involvement of the mitochondrial pathway in NMDA-mediated neuronal survival.

    abstract::N-methyl-d-aspartate (NMDA) subtype of glutamate receptors plays an important role during brain development and receptor activation has been shown to promote neuronal survival. The mechanisms by which NMDA increases neuronal survival are not fully understood. Here, we show that treatment with low concentrations of NMD...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Korhonen L,Näpänkangas U,Steen H,Chen Y,Martinez R,Lindholm D

    更新日期:2004-05-01 00:00:00

  • Serum effects on the in vitro differentiation of sea urchin micromeres.

    abstract::When micromeres isolated from the 16-cell stage of Strongylocentrotus purpuratus are cultured in sea water containing 3.5% horse serum, they produce spicules at approximately the same time as in normal development. The serum requirement of the micromeres has been investigated by adding serum at varying intervals after...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: McCarthy RA,Spiegel M

    更新日期:1983-12-01 00:00:00

  • Purification and characterization of integrin alpha 9 beta 1.

    abstract::A new beta 1-containing integrin was isolated from rat liver by affinity chromatography on Sepharose conjugated with the peptide GRGDSPC. The interaction was weakened but not abolished when the arginine and/or aspartic acid in the peptide were replaced with lysine and glutamic acid, respectively. In contrast, the cyst...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Forsberg E,Ek B,Engström A,Johansson S

    更新日期:1994-07-01 00:00:00

  • Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression.

    abstract::A novel protein that associates with interphase nucleus and mitotic apparatus (INMAP) was identified by screening HeLa cDNA expression library with an autoimmune serum followed by tandem mass spectrometry. Its complete cDNA sequence of 1.818 kb encodes 343 amino acids with predicted molecular mass of 38.2 kDa and nume...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Shen E,Lei Y,Liu Q,Zheng Y,Song C,Marc J,Wang Y,Sun L,Liang Q

    更新日期:2009-04-15 00:00:00

  • Mouse macrophage polarity and ROCK1 activity depend on RhoA and non-apoptotic Caspase 3.

    abstract::The macrophages have different subtypes with different functions in immune response and disease. It has been generally accepted that M1 macrophages are responsible for stimulation of immune system and inflammation while M2 macrophages play a role in tissue repair. Irrespective of the type, macrophage functions depend ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Liu Y,Minze LJ,Mumma L,Li XC,Ghobrial RM,Kloc M

    更新日期:2016-02-15 00:00:00

  • Modulation of the phosphorylation of glucose-regulated protein, GRP78, by transformation and inhibition of glycosylation.

    abstract::The phosphorylation of glucose-regulated protein, GRP78, is thought to be involved in the regulation of the binding function of GRP78 to immunoglobulin heavy chains. The phosphorylation of GRP78 proceeded faster in transformed cells than in normal cells, whereas the levels of GRP78 synthesis and accumulation were simi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Satoh M,Nakai A,Sokawa Y,Hirayoshi K,Nagata K

    更新日期:1993-03-01 00:00:00

  • Ciliary neurotrophic factor combined with soluble receptor inhibits synthesis of proinflammatory cytokines and prostaglandin-E2 in vitro.

    abstract::In human peripheral blood mononuclear cells, ciliary neurotrophic factor (CNTF) weakly suppressed endotoxin-induced interleukin (IL)-1 and prostaglandin E2(PGE2). Suppression of PGE2 and IL-8 synthesis was significantly greater (up to 42.6%, P < 0.05) by adding a 10-fold molar excess of soluble CNTF receptor (sCNTFR a...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Shapiro L,Panayotatos N,Meydani SN,Wu D,Dinarello CA

    更新日期:1994-11-01 00:00:00

  • Human Kupffer cell recognition and phagocytosis of apoptotic peripheral blood lymphocytes.

    abstract::Cells undergoing apoptosis are recognized and rapidly phagocytosed by macrophages before their degradation, thus preventing the inflammatory reaction and protecting tissues from the damaging effects of released potentially harmful intracellular contents. In spite of growing interest in the mechanisms leading to the en...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Falasca L,Bergamini A,Serafino A,Balabaud C,Dini L

    更新日期:1996-04-10 00:00:00

  • Cellular localization and expression of template-activating factor I in different cell types.

    abstract::Template-activating factors I (TAF-I) alpha and beta have been identified as chromatin remodeling factors from human HeLa cells. TAF-I beta corresponds to the protein encoded by the set gene, which was found in an acute undifferentiated leukemia as a fusion version with the can gene via chromosomal translocation. To d...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nagata K,Saito S,Okuwaki M,Kawase H,Furuya A,Kusano A,Hanai N,Okuda A,Kikuchi A

    更新日期:1998-05-01 00:00:00

  • Parallels between immune driven-hematopoiesis and T cell activation: 3 signals that relay inflammatory stress to the bone marrow.

    abstract::Quiescence, self-renewal, lineage commitment and differentiation of hematopoietic stem cells (HSCs) towards fully mature blood cells are a complex process that involves both intrinsic and extrinsic signals. During steady-state conditions, most hematopoietic signals are provided by various resident cells inside the bon...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Libregts SF,Nolte MA

    更新日期:2014-12-10 00:00:00

  • CP beta3, a novel isoform of an actin-binding protein, is a component of the cytoskeletal calyx of the mammalian sperm head.

    abstract::In the mammalian sperm head, the nucleus is tightly associated with the calyx, a cell type-specific cytoskeletal structure. Previously, we have identified and characterized some basic proteins such as calicin and cylicins I and II as major calyx components of bovine and human spermatids and spermatozoa. Surprisingly w...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: von Bülow M,Rackwitz HR,Zimbelmann R,Franke WW

    更新日期:1997-05-25 00:00:00

  • Involvement of JNK signaling in IL4-induced M2 macrophage polarization.

    abstract::It has been generally accepted that alternatively activated macrophages (M2), which can be induced by type 2 cytokines such as IL-4, is responsible for tissue repair. However, the function of JNK in IL-4-induced M2 macrophage polarization remains unclear. Here, we demonstrated that M0 macrophages can be polarized into...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hao J,Hu Y,Li Y,Zhou Q,Lv X

    更新日期:2017-08-15 00:00:00

  • Molecular segments of protein Tpr that confer nuclear targeting and association with the nuclear pore complex.

    abstract::Tpr is a 267-kDa protein of unknown function recently identified as a constitutive component of the nuclear pore complex (NPC2)-attached intranuclear filaments. Secondary structure predictions suggest that the protein is divided into a large, coiled-coil forming aminoterminal domain and a shorter, highly acidic carbox...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Cordes VC,Hase ME,Müller L

    更新日期:1998-11-25 00:00:00

  • Growth and differentiation potential of main- and side-population cells derived from murine skeletal muscle.

    abstract::Skeletal muscle-derived CD34+/45- (Sk-34) cells were identified as a new candidate for stem cells. However, the relationship between Sk-34 cells and side-population (SP) cells is unknown. Here, we demonstrate that Sk-34 cells prepared from murine skeletal muscles consist wholly of main-population (MP) cells. The Sk-34...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tamaki T,Akatsuka A,Okada Y,Matsuzaki Y,Okano H,Kimura M

    更新日期:2003-11-15 00:00:00

  • Fusion-mediated implantation of band 3 into living cells. A new system to study degradation of membrane proteins.

    abstract::Sendai virus (SV) glycoproteins were co-reconstituted with the erythrocyte membrane polypeptide designated as band 3 (B3). Fusion of the hybrid vesicles, SV-B3, with L, HeLa and Friend erythroleukemic (FEL) cells led to fusion-mediated implantation of the erythrocyte band 3 into the plasma membranes of the above cells...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Beigel M,Loyter A

    更新日期:1983-10-01 00:00:00

  • Synthesis and fate of keratins 8 and 18 in nonepithelial cells transfected with cDNA.

    abstract::To study the assembly of intermediate filaments in vivo we have transfected fibroblast cell lines with the cDNAs coding for keratins 8 and 18 under the control of the promoter of the SV40 early region and followed keratin expression by RNA hybridization, two-dimensional gel electrophoresis, and immunofluorescence anal...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Domenjoud L,Jorcano JL,Breuer B,Alonso A

    更新日期:1988-12-01 00:00:00

  • Reserpine-induced alterations in mucus production and calmodulin-binding proteins in a human epithelial cell line.

    abstract::The characterization of a mucus-producing human cell line (HC-84) derived from a colon carcinoma and its response to in vitro reserpine treatment is reported. Mucous granules were demonstrated within these cells on the basis of electron microscopic examination and incorporation of [3H]glucosamine with subsequent autor...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Brady RC,Karnaky KJ Jr,Dedman JR

    更新日期:1984-01-01 00:00:00

  • Hypoxia, pseudohypoxia and cellular differentiation.

    abstract::Tumor hypoxia correlates to aggressive disease, and while this is explained by a variety of factors, one clue to understand this phenomena was the finding that hypoxia induces a de-differentiated, stem cell-like phenotype in neuroblastoma and breast tumor cells. The hypoxia inducible transcription factors (HIFs) are r...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Mohlin S,Wigerup C,Jögi A,Påhlman S

    更新日期:2017-07-15 00:00:00

  • Role of cytoskeletal elements in the retractile activity of human skin fibroblasts.

    abstract::Giant axonal neuropathy skin fibroblasts, which are characterized by a selective and partial disorganization of vimentin filaments [1] exhibited, when compared with normal skin fibroblasts, less fibrin clot retractile (FCR) activity and spreading within the fibrin clot both during active growth and resting stage. Skin...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Azzarone B,Macieira-Coelho A

    更新日期:1984-11-01 00:00:00

  • Migration potential and gene expression profile of human mesenchymal stem cells induced by CCL25.

    abstract::Recruitment of mesenchymal stem cells (MSC) to tissue damages is a promising approach for in situ tissue regeneration. The physiological mechanisms and regulatory processes of MSC trafficking to injured tissue remain poorly understood. However, the pivotal role of chemokines in MSC recruitment has already been shown. ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Binger T,Stich S,Andreas K,Kaps C,Sezer O,Notter M,Sittinger M,Ringe J

    更新日期:2009-05-01 00:00:00

  • The reversal of paraquat-induced mitochondria-mediated apoptosis by cycloartenyl ferulate, the important role of Nrf2 pathway.

    abstract::In this study, we demonstrate the protective effects of Cycloartenyl ferulate (CF) against Paraquat (PQ)-induced cytotoxicity and elucidate the underlying molecular mechanisms. The results show that, CF could reverse the PQ-induced growth inhibition and release of lactate dehydrogenase in HK-2 human proximal tubular c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hong GL,Liu JM,Zhao GJ,Wang L,Liang G,Wu B,Li MF,Qiu QM,Lu ZQ

    更新日期:2013-11-01 00:00:00

  • Colocalization of nestin and vimentin/desmin in skeletal muscle cells demonstrated by three-dimensional fluorescence digital imaging microscopy.

    abstract::During skeletal muscle development three intermediate filament proteins are expressed: nestin, vimentin, and desmin. Vimentin and desmin belong to the class III intermediate filaments and are closely related to each other, whereas nestin is a more distantly related, class VI, intermediate filament. It was previously o...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sjöberg G,Jiang WQ,Ringertz NR,Lendahl U,Sejersen T

    更新日期:1994-10-01 00:00:00