Abstract:
:Signalling through the fibroblast growth factor family (FGF) of ligands is essential for normal mammalian embryonic development. At a cellular level, many details of the molecular basis of the signal transduction process have been uncovered, but our knowledge of the identity of the downstream effectors of the FGF signal in the developing embryo remains limited. We have used two independent approaches to begin to identify downstream targets of FGF signalling in the embryo: (1). a gene trap approach and (2). cDNA subtraction, using mouse embryonic stem (ES) cells as a cellular system representative of an early window on the developing embryo. Both approaches led to the identification of a number of targets of FGF signalling, and we provide data to show that the chaperone Mrj, the tumour antigen Tum, collapsin mediator response protein Crmp, a novel transcriptional repressor Nac1 and ribophorin are all differentially regulated following FGF signalling. Independent gene trapping of Mrj previously indicated a role for the gene in embryogenesis [Development 126 (1999) 1247], and we present transcript data implicating a number of the newly isolated FGF target genes in different embryonic processes.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Tateossian H,Powles N,Dickinson R,Ficker M,Maconochie Mdoi
10.1016/j.yexcr.2003.08.008keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
101-14issue
1eissn
0014-4827issn
1090-2422pii
S0014482703004543journal_volume
292pub_type
杂志文章abstract::The bone marrow represents an easy accessible source of adult stem cells suitable for various cell based therapies. Several studies in recent years suggested the existence of pluripotent stem cells within bone marrow stem cells (BMSC) expressing marker proteins of both embryonic and tissue committed stem cells. These ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.10.007
更新日期:2009-01-01 00:00:00
abstract::The interaction of fluorescein-labeled nerve growth factor (NGF) with human melanoma cells (A875) has been studied in order to assess better methodology for rigorous NGF binding studies. The NGF was modified at a single carboxyl group with iodoacetamidofluorescein after reaction with carbodiimide and cystamine. The mo...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1012
更新日期:1994-01-01 00:00:00
abstract::Fragments of rough endoplasmic reticulum and Golgi complex purified from rat liver homogenates were injected into Xenopus oocytes and the sites of microinjection analysed by electron microscopy at different times post-injection. The in vivo incubated fragments were located by their proximity to a microinjection vacuol...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90495-7
更新日期:1986-10-01 00:00:00
abstract::Lithium exerts neuroprotective actions that involve the inhibition of glycogen synthase kinase-3beta (GSK-3beta). Otherwise, recent studies suggest that sustained GSK-3beta inhibition is a hallmark of tumorigenesis. In this context, the present study was undertaken to examine whether lithium modulated cancer cell sens...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.08.001
更新日期:2004-11-01 00:00:00
abstract::The CDC25Mm gene codes for Ras-guanine nucleotide exchange factors. Four different full-length cDNA clones derived from the same gene and coding for proteins of different sizes that have in common the last 661 amino acids have been isolated from mouse brain. In order to investigate the expression of the products of th...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1048
更新日期:1994-02-01 00:00:00
abstract::The osteoblast-like MC3T3-E1 cell line provides an excellent in vitro model of bone development. This system undergoes three orderly time-dependent phases characterized by proliferating preosteoblasts, matrix accumulation by postmitotic differentiating osteoblasts, and mineralization of the matrix, which results in th...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.4879
更新日期:2000-05-25 00:00:00
abstract::Maturation of MC3T3-E1 osteoblast cells in vitro can be divided into three major stages, namely, proliferation, differentiation, and mineralization. Our microarray analysis identified genes differentially expressed between proliferating and differentiated MC3T3-E1 osteoblastic cells. Immunohistochemical analyses of RN...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.10.031
更新日期:2005-03-10 00:00:00
abstract::Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1052
更新日期:1993-03-01 00:00:00
abstract::Hypoxia/reoxygenation (H/R) injury is characterized by microfilament reorganization in cardiomyocytes. Previous studies have shown that myofibrillogenesis regulator-1 (MR-1) is expressed in the myocardium and promotes actin organization in cardiomyocytes. The purpose of this study was to investigate the role of MR-1 i...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2015.05.026
更新日期:2015-10-01 00:00:00
abstract::Chronic lymphocytic leukemia (CLL) results from the uncontrolled proliferation and accumulation of B-1 cells, many of which demonstrate self-reactivity. The response of B-1 cells to mitogen after undergoing malignant transformation is still unclear. Using our established malignant B-1 cell lines derived from the NZB m...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.5122
更新日期:2001-04-01 00:00:00
abstract::The ccl mutation in Paramecium tetraurelia reversibly and rapidly blocks cell cycle progression and DNA synthesis at the restrictive temperature. Progression through the cell cycle is blocked during both the G1 and S portions of the cell cycle, while at the restrictive temperature there is neither residual cell cycle ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90220-9
更新日期:1984-12-01 00:00:00
abstract::Osteoclasts are postmitotic, multinucleated giant cells generated by the fusion of hematopoietic mononuclear precursors from the monocyte-macrophage lineage. In culture, adherent macrophages from blood-derived monocytes grow, gather, and fuse together to form multinucleated osteoclast-like cells. These events are cont...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4526
更新日期:1999-08-01 00:00:00
abstract::Effector functions in tumor resistance by dendritic cells (DCs) are less well characterized. In this study, we describe that the murine DCs upon stimulation with recombinant IL-15 in vitro or in vivo, expresses TNF superfamily member TRAIL which mediates cytotoxicity and growth inhibition against a murine lymphoma cal...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.08.012
更新日期:2014-10-01 00:00:00
abstract::Eighteen cell lines contaminated with various mycoplasmas have been treated with hyperimmune sera and mycoplasmas have been eradicated from all. After treatment the cell lines have been observed for a least one year and they are still free from mycoplasma contamination as ascertained by four independent mycoplasma det...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90502-6
更新日期:1985-11-01 00:00:00
abstract::Shp-2, a widely expressed cytoplasmic tyrosine phosphatase with two src-homology 2 (SH2) domains, has received much attention in the signal transduction field recently. Significant progress has been made in understanding the structure and function of this phosphatase, together with its Drosophila homologue, Corkscrew,...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1006/excr.1999.4668
更新日期:1999-11-25 00:00:00
abstract::Gp70 is an esterase originally called crystal protein because of its presence in crystalline structures in aggregation-competent Dictyostelium discoideum cells. Although postulated to break down spore coats, the function of gp70 in vivo was incompletely investigated. Our immunolocalization and biochemical studies of v...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.5055
更新日期:2000-12-15 00:00:00
abstract::Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated nucleotides has been recently proposed as a means of analyzing DNA replication and detecting proliferating cells. The presence of numerous in situ DNA strand breaks is also an inherent feature of apoptotic cells. T...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0004
更新日期:1996-01-10 00:00:00
abstract::Abrupt developmental changes occur in structural form and function of connexin (Cx) channels in the mouse mammary gland. Microarray study shows that the principal connexin isoform in epithelial cells during pregnancy is Cx26, up-regulated and persisting from the virgin. After parturition, there is rapid induction of C...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.05.003
更新日期:2004-08-15 00:00:00
abstract::The program of biochemical and molecular events necessary for commitment to erythroid cell differentiation is particularly well characterized in murine Friend erythroleukemia cell lines. Commitment to hemoglobin synthesis in response to a variety of chemical inducers, including hexamethylene bisacetamide and dimethyl ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4312
更新日期:1999-02-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) are frequently used as a therapeutic, but reliable imaging technique to longitudinally evaluate the engraftment of transplanted cells is inadequate. For magnetic resonance imaging (MRI), it is essential to understand the technical competence of in vitro stem cells labeling with iron oxide...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2016.12.025
更新日期:2017-02-01 00:00:00
abstract::The enzyme endothelial Nitric Oxide Synthase (eNOS) is involved in key physiological and pathological processes, including cell motility and apoptosis. It is widely believed that at the cell surface eNOS is localized in caveolae, where caveolin-1 negatively regulates its activity, however, there are still uncertaintie...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.12.014
更新日期:2006-04-01 00:00:00
abstract::We have studied the morphological alterations of the lysosomal compartment in rat hepatocytes following intraperitoneal administration of leupeptin, using electron microscopy and cytochemical techniques. At 30 min after the injection, autophagic vacuoles (autophagosomes and autolysosomes), containing cytoplasmic organ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(83)90436-6
更新日期:1983-03-01 00:00:00
abstract::It is known that DNA fragmentation during apoptosis is controlled by a number of factors, a crucial step being the caspase-operated cleavage of ICAD, the DNase inhibitor. We have previously demonstrated that hydrogen peroxide-treated lymphocytes undergo apoptosis without formation of a DNA ladder; however, the use of ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5222
更新日期:2001-06-10 00:00:00
abstract::The tumor-associated immune response is governed by the signalling events of various regulatory molecules, one of which is the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). In conventional T cells, CTLA-4 could outcompete CD28 in binding to CD80/86 but does not produce a co-stimulatory signal, resulting in T c...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.09.030
更新日期:2017-11-15 00:00:00
abstract::The highly purified, luminal domain of rat lung endothelial plasma membranes was used as an immunogen to obtain monoclonal antibodies to the endothelial cell surface. The procedure was highly efficient, yielding antibodies which recognize three seemingly novel endothelial integral membrane glycoproteins of molecular w...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3490
更新日期:1997-04-10 00:00:00
abstract::An adenocarcinoma cell line which has the ability to arrest in G0 phase under exhausted culture conditions was established. Using the cell line, we investigated the expression of cell cycle-associated proteins including cdc2, cdk2, cyclin A, cyclin Dl, and Rb during entry into or withdrawal from the cell cycle. MAP ki...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0159
更新日期:1996-05-25 00:00:00
abstract::Two genes, KIN1 and KIN2, were isolated in Saccharomyces cerevisiae by their homology to the protein kinase family of viral oncogenes. In previous studies, we identified the yeast KIN1 gene product (pp145KIN1) and KIN2 gene product (pp145KIN2) as 145 kDa phosphoproteins with serine/threonine protein kinase activity. W...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1177
更新日期:1994-07-01 00:00:00
abstract::Centrosomes, the main microtubule-organizing centers (MTOCs) in most animal cells, are important for many cellular activities such as assembly of the mitotic spindle, establishment of cell polarity, and cell movement. In nuclear transfer (NT), MTOCs that are located at the poles of the meiotic spindle are removed from...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.02.009
更新日期:2005-05-15 00:00:00
abstract::Mitochondrial DNA depleted (ρ(0)) human skin fibroblasts (HSF) with suppressed oxidative phosphorylation were characterized by significant changes in the expression of 2100 nuclear genes, encoding numerous protein classes, in NF-κB and STAT3 signaling pathways, and by decreased activity of mitochondrial death pathway,...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2011.03.012
更新日期:2011-07-01 00:00:00
abstract::Pathological cardiac hypertrophy is a maladaptive response in a variety of organic heart disease (OHD), which is characterized by mitochondrial dysfunction that results from disturbed energy metabolism. SIRT3, a mitochondria-localized sirtuin, regulates global mitochondrial lysine acetylation and preserves mitochondri...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2016.07.006
更新日期:2016-10-01 00:00:00