Determination of downstream targets of FGF signalling using gene trap and cDNA subtractive approaches.

Abstract:

:Signalling through the fibroblast growth factor family (FGF) of ligands is essential for normal mammalian embryonic development. At a cellular level, many details of the molecular basis of the signal transduction process have been uncovered, but our knowledge of the identity of the downstream effectors of the FGF signal in the developing embryo remains limited. We have used two independent approaches to begin to identify downstream targets of FGF signalling in the embryo: (1). a gene trap approach and (2). cDNA subtraction, using mouse embryonic stem (ES) cells as a cellular system representative of an early window on the developing embryo. Both approaches led to the identification of a number of targets of FGF signalling, and we provide data to show that the chaperone Mrj, the tumour antigen Tum, collapsin mediator response protein Crmp, a novel transcriptional repressor Nac1 and ribophorin are all differentially regulated following FGF signalling. Independent gene trapping of Mrj previously indicated a role for the gene in embryogenesis [Development 126 (1999) 1247], and we present transcript data implicating a number of the newly isolated FGF target genes in different embryonic processes.

journal_name

Exp Cell Res

authors

Tateossian H,Powles N,Dickinson R,Ficker M,Maconochie M

doi

10.1016/j.yexcr.2003.08.008

keywords:

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

101-14

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014482703004543

journal_volume

292

pub_type

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