Abstract:
:The enzyme endothelial Nitric Oxide Synthase (eNOS) is involved in key physiological and pathological processes, including cell motility and apoptosis. It is widely believed that at the cell surface eNOS is localized in caveolae, where caveolin-1 negatively regulates its activity, however, there are still uncertainties on its intracellular distribution. Here, we applied high resolution confocal microscopy to investigate the surface distribution of eNOS in transfected HeLa cells and in human umbilical vein endothelial cells (HUVEC) endogenously expressing the enzyme. In confluent and non-confluent HUVEC and HeLa cells, we failed to detect substantial colocalization between eNOS and caveolin-1 at the cell surface. Instead, in non-confluent cells, eNOS was concentrated in ruffles and at the leading edge of migrating cells, colocalizing with actin filaments and with the raft marker ganglioside G(M1), and well segregated from caveolin-1, which was restricted to the posterior region of the cells. Treatments that disrupted microfilaments caused loss of eNOS from the cell surface and decreased Ca(2+)-stimulated activity, suggesting a role of the cytoskeleton in the localization and function of the enzyme. Our results provide a morphological correlate for the role of eNOS in cell migration and raise questions on the site of interaction between eNOS and caveolin-1.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Bulotta S,Cerullo A,Barsacchi R,De Palma C,Rotiroti D,Clementi E,Borgese Ndoi
10.1016/j.yexcr.2005.12.014keywords:
subject
Has Abstractpub_date
2006-04-01 00:00:00pages
877-89issue
6eissn
0014-4827issn
1090-2422pii
S0014-4827(05)00582-3journal_volume
312pub_type
杂志文章abstract::Melanoma formation in the teleost fish Xiphophorus is caused by the uncontrolled activity of the genetically defined tumor locus Tu. The critical component of this locus is the Xmrk oncogene encoding a subclass I receptor tyrosine kinase. Overexpression and constitutive activation of the Xmrk receptor triggers a set o...
journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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doi:10.1006/excr.1998.4234
更新日期:1998-11-25 00:00:00
abstract:BACKGROUND AND OBJECTIVE:In recent years, immunotherapy for the treatment of tumors have been established. Dendritic cells (DCs) are extremely efficient and professional antigen presenting cells (APCs), which are an important target for immune therapeutic interventions in cancer. In present study, we investigated wheth...
journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2016-12-10 00:00:00