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Effects of colchicine on cardiac cell function indicate possible role for membrane surface tubulin.

Abstract：

:The effects of the tubulin-binding drug colchicine on cultured neonate cardiac cell function were investigated. Application of low doses of colchicine (but not lumicolchicine) caused an early reversible increase in beating rate with a concomitant decrease in amplitude. Treatment of the cells with trypsin at a dose that removes surface tubulin but does not inhibit spontaneous beating, diminished the colchicine effect. Surface radio-iodination of the live cultures followed by two-dimensional gel electrophoresis and radioautography revealed that two spots were heavily labeled. These spots co-migrated with purified brain tubulin. Fibroblasts derived from the cardiac cultures did not label over the tubulin spots. Trypsin treatment removed the presumptive tubulin from the radioautographs but only removed the most basic portion of the alpha-tubulin spot from the stained gel. These results are consistent with a surface membrane role for an iso-form of tubulin in neonate cardiac cells.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Lampidis TJ,Trevorrow KW,Rubin RW

doi

10.1016/0014-4827(86)90044-3

subject

Has Abstract

pub_date

1986-06-01 00:00:00

pages

463-70

issue

2

eissn

0014-4827

issn

1090-2422

journal_volume

164

pub_type

杂志文章

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