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Spontaneous apoptosis of thymocytes is uncoupled with progression through the cell cycle.

Abstract：

:Most developing lymphocytes spontaneously die in the thymus during positive and negative selection of the T cell repertoire. By evaluating the expression of the proliferation antigens Ki-67 and PCNA, we demonstrated here that more than 95% of thymocytes are potentially proliferating. The coincidence within the same cell population of death and proliferation is thus apparent in developing thymocytes. Using dual-parameter cytometric techniques to evaluate in single cells the amount of DNA versus light-scattering values, we found that spontaneous thymocyte apoptosis occurs with similar frequency in all the cycle phases, whereas apoptosis induced by the anti-topoisomerase-II, etoposide (which is the consequence of irreversible DNA damage), takes place with higher frequency in S and G2 phases (i.e., in those cycle phases in which DNA is subjected to torsional constraints). The capability of thymocytes to enter apoptosis was also monitored by digesting DNA in situ with DNase I (a nuclease that cleaves DNA mimicking the nuclear damage common to most apoptotic suicides). We also show that endonuclease-mediated DNA digestion occurs to a similar extent in cells with different DNA contents, i.e., in cycle phases in which the superstructural organization of chromatin is markedly different.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Pellicciari C,Bottone MG,Schaack V,Barni S,Manfredi AA

doi

10.1006/excr.1996.0382

subject

Has Abstract

pub_date

1996-12-15 00:00:00

pages

370-7

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(96)90382-1

journal_volume

229

pub_type

杂志文章

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