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R5, a neutralizing antibody to Robo1, suppresses breast cancer growth and metastasis by inhibiting angiogenesis via down-regulating filamin A.

Abstract：

:Breast cancer (BC) is one of the most common cancers among women in both developed and developing countries with a rising incidence. Using the MMTV-PyMT transgenic mouse model and xenografted breast cancer model, we found that R5, a neutralizing antibody to Robo1, significantly inhibited BC growth and metastasis. Angiogenesis is involved in the growth and metastasis of BC. Interestingly, R5 significantly decreases microvessel density in BC tissues, and inhibits blood vessel formation and development in in vivo chick embryo chorioallantoic membrane (CAM), yolk sac membrane (YSM) and Matrigel plug models. To investigate whether its anti-breast cancer efficacy is ascribed to its direct antiangiogenic properties, xenografted breast cancer model on CAM was established. Furthermore, R5 significantly reduces the tube formation of the vascular plexus on xenografted breast tumor on CAM. R5 also suppresses the migration and the tubular structure formation of human umbilical vein endothelial cells (HUVECs) by down-regulating the expression of filamin A (FLNA). These findings show that R5 has the potential to be a promising agent for the treatment of BC by suppressing the tumor-induced angiogenesis.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Li Q,Cao J,He Y,Liu X,Mao G,Wei B,Liao S,Zhang Q,Li J,Zheng L,Wang L,Qi C

doi

10.1016/j.yexcr.2019.111756

subject

Has Abstract

pub_date

2020-02-01 00:00:00

pages

111756

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(19)30639-1

journal_volume

387

pub_type

杂志文章

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