当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > MiR-212-3p inhibits LPS-induced inflammatory response through targeting HMGB1 in murine macrophages.

MiR-212-3p inhibits LPS-induced inflammatory response through targeting HMGB1 in murine macrophages.

Abstract：

:Sepsis is a major cause of mortality in seriously ill patients characterized by a series of severe systemic inflammatory responses due to an infection. Thus, there is a critically need to search more accurate biomarkers and targets for diagnosis and treatment of sepsis. Our study showed that miR-212-3p was up-regulated in LPS-treated macrophage RAW264.7 cells. Overexpression of miR-212-3p in RAW264.7 cells led to suppression of pro-inflammatory cytokines (TNF-α and IL-6) induced by LPS. Bioinformatic predictions and experimental researches both revealed that HMGB1 was a direct target of miR-212-3p. Meanwhile, the results showed that overexpression of miR-212-3p inhibited the cytoplasmic translocation of HMGB1 in LPS-induced RAW264.7 cells. Subsequently, transfection of the pcDNA3.1/HMGB1 plasmid, which produced HMGB1 overexpression, exhibited similar effects as the LPS-induced macrophage inflammatory response and markedly activated the MAPKs including p38, ERK and JNK phosphorylation. Furthermore, we also found that the phosphorylation of p38 MAPK and ERK was downregulated by miR-212-3p mimics upon LPS injection. In conclusion, these results reveal that miR-212-3p directly targets HMGB1 to suppress inflammatory response in LPS-induced RAW264.7 cells. All our findings indicate that miR-212-3p may act as a potential pharmacological target for promising and effective therapeutic intervention in microbial infection in the future.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Chen W,Ma X,Zhang P,Li Q,Liang X,Liu J

doi

10.1016/j.yexcr.2016.12.008

subject

Has Abstract

pub_date

2017-01-15 00:00:00

pages

318-326

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(16)30407-4

journal_volume

350

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Small interfering RNA targeting ILK inhibits metastasis in human tongue cancer cells through repression of epithelial-to-mesenchymal transition.

    abstract：:Integrin-linked kinase (ILK) is a multifunctional serine/threonine kinase. Accumulating evidences suggest that ILK are involved in cell-matrix interactions, cell proliferation, invasion, migration, angiogenesis and Epithelial-mesenchymal transition (EMT). However, the underlying mechanisms remain largely unknown. EMT ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.05.014

    authors： Xing Y,Qi J,Deng S,Wang C,Zhang L,Chen J

    更新日期：2013-08-01 00:00:00

  • SIRT1 and microRNAs: The role in breast, lung and prostate cancers.

    abstract：:Breast cancer and prostate cancer are the most common malignant tumors in female and men, respectively. Furthermore, lung cancer is the leading cause of cancer deaths worldwide. It is an emergency to develop a powerful strategy to treat these threatening cancers more effectively, because of low efficacy and high rates...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2018.03.023

    authors： Karbasforooshan H,Roohbakhsh A,Karimi G

    更新日期：2018-06-01 00:00:00

  • Characterization and response of newly developed high-grade glioma cultures to the tyrosine kinase inhibitors, erlotinib, gefitinib and imatinib.

    abstract：:High-grade gliomas (HGG), are the most common aggressive brain tumours in adults. Inhibitors targeting growth factor signalling pathways in glioma have shown a low clinical response rate. To accurately evaluate response to targeted therapies further in vitro studies are necessary. Growth factor pathway expression usin...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2012.01.014

    authors： Kinsella P,Howley R,Doolan P,Clarke C,Madden SF,Clynes M,Farrell M,Amberger-Murphy V

    更新日期：2012-03-10 00:00:00

  • Axin is expressed in mitochondria and suppresses mitochondrial ATP synthesis in HeLa cells.

    abstract：:Many recent studies have revealed that axin is involved in numerous cellular functions beyond the negative regulation of β-catenin-dependent Wnt signaling. Previously, an association of ectopic axin with mitochondria was observed. In an effort to investigate the relationship between axin and mitochondria, we found tha...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.12.003

    authors： Shin JH,Kim HW,Rhyu IJ,Kee SH

    更新日期：2016-01-01 00:00:00

  • A SUV39H1-low chromatin state characterises and promotes migratory properties of cervical cancer cells.

    abstract：:Metastatic progression is a major cause of mortality in cervical cancers, but factors regulating migratory and pre-metastatic cell populations remain poorly understood. Here, we sought to assess whether a SUV39H1-low chromatin state promotes migratory cell populations in cervical cancers, using meta-analysis of data f...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.02.010

    authors： Rodrigues C,Pattabiraman C,Vijaykumar A,Arora R,Narayana SM,Kumar RV,Notani D,Varga-Weisz P,Krishna S

    更新日期：2019-05-15 00:00:00

  • ATP/P2X7-NLRP3 axis of dendritic cells participates in the regulation of airway inflammation and hyper-responsiveness in asthma by mediating HMGB1 expression and secretion.

    abstract：:The ATP/P2X7 axis of dendritic cells (DCs) mediates the activation of NLRP3 inflammasome and promotes secretion of interleukin (IL)-1β and IL-18 to induce T helper (Th) 2, Th17 differentiation in the pathogenesis of asthma. NLRP3 inflammasome also regulates high mobility protein 1 (HMGB1) release in DCs. Recent studie...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.03.002

    authors： Li R,Wang J,Li R,Zhu F,Xu W,Zha G,He G,Cao H,Wang Y,Yang J

    更新日期：2018-05-01 00:00:00

  • Induction of neurite outgrowth in PC12 cells by gamma-lactam-related compounds via Ras-MAP kinase signaling pathway independent mechanism.

    abstract：:Rat pheochromocytoma cells, PC12 cells, undergo differentiation in response to nerve growth factor (NGF). Although the Ras-MAP kinase signaling pathway has been shown to play a central role in the response to NGF, the precise mechanism which induces differentiation remains unclarified. Recently, several gamma-lactam-r...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3615

    authors： Yao R,Osada H

    更新日期：1997-08-01 00:00:00

  • Measurement of cell-monolayer adhesion in cells transfected with N-CAM cDNAs.

    abstract：:To measure the adhesion of cells expressing the neural cell adhesion molecule N-CAM, mouse Lmtk- fibroblast cells were transfected by a calcium phosphate precipitation technique with eucaryotic expression vectors encoding N-CAM polypeptides. We obtained cell lines expressing the 140-kDa transmembrane isoform of N-CAM ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1041

    authors： Woo MK,Murray BA

    更新日期：1993-02-01 00:00:00

  • CD25-expressing Th17 cells mediate CD8+ T cell suppression in CTLA-4 dependent mechanisms in pancreatic ductal adenocarcinoma.

    abstract：:The tumor-associated immune response is governed by the signalling events of various regulatory molecules, one of which is the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). In conventional T cells, CTLA-4 could outcompete CD28 in binding to CD80/86 but does not produce a co-stimulatory signal, resulting in T c...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.09.030

    authors： Lang C,Wang J,Chen L

    更新日期：2017-11-15 00:00:00

  • Increased mitochondrial-encoded gene transcription in immortal DF-1 cells.

    abstract：:We have established, in continuous cell culture, a spontaneously immortalized chicken embryo fibroblast (CEF) cell line (DF-1) as well as several other immortal CEF cell lines. The immortal DF-1 cells divided more rapidly than primary and other immortal CEF cells. To identify the genes involved in rapidly dividing DF-...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5207

    authors： Kim H,You S,Kim IJ,Farris J,Foster LK,Foster DN

    更新日期：2001-05-01 00:00:00

  • Pretreatment with oleic acid accelerates the entrance into the mitotic cycle of EGF-stimulated fibroblasts.

    abstract：:We have previously demonstrated that pretreatment of several cell lines with cis-unsaturated fatty acids, like oleic acid, blocks epidermal growth factor (EGF)-induced early ionic signals, and in particular the [Ca2+]i rise. In the present work we show that this blockade does not alter EGF-stimulated cellular prolifer...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1204

    authors： Zugaza JL,Casabiell XA,Bokser L,Eiras A,Beiras A,Casanueva FF

    更新日期：1995-07-01 00:00:00

  • Protein phosphatase 2A is involved in the regulation of protein kinase A signaling pathway during in vitro chondrogenesis.

    abstract：:We have evaluated the importance of the Ser/Thr protein phosphorylation and dephosphorylation for chondrogenesis in high-density chicken limb bud mesenchymal cell cultures (HDCs) by using H89, a cell-permeable protein kinase inhibitor, and okadaic acid (OA), a phosphoprotein phosphatase (PP)-specific inhibitor molecul...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5487

    authors： Zákány R,Szucs K,Bakó E,Felszeghy S,Czifra G,Bíró T,Módis L,Gergely P

    更新日期：2002-04-15 00:00:00

  • Immortalization in a normal foreskin fibroblast culture following transduction of cyclin A2 or cdk1 genes in retroviral vectors.

    abstract：:Human diploid fibroblasts (HDF) rarely, if ever, undergo spontaneous transformation to an immortalized cell type. Here we report the immortalization of an HDF cell line following transduction with cyclin A2 or cdk1 human genes via retroviral vectors. Fluorescence in situ hybridization (FISH) studies using the retrovir...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2003.11.030

    authors： Luo P,Tresini M,Cristofalo V,Chen X,Saulewicz A,Gray MD,Banker DE,Klingelhutz AL,Ohtsubo M,Takihara Y,Norwood TH

    更新日期：2004-04-01 00:00:00

  • Disruption of IGF-1R signaling increases TRAIL-induced apoptosis: a new potential therapy for the treatment of melanoma.

    abstract：:Resistance of cancer cells to apoptosis is dependent on a balance of multiple genetic and epigenetic mechanisms, which up-regulate efficacy of the surviving growth factor-receptor signaling pathways and suppress death-receptor signaling pathways. The Insulin-like Growth Factor-1 Receptor (IGF-1R) signaling pathway is ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.04.014

    authors： Karasic TB,Hei TK,Ivanov VN

    更新日期：2010-07-15 00:00:00

  • CD21lo/medCD27+ proinflammatory B cells are enriched in breast cancer patients and promote antitumor T cell responses.

    abstract：:Breast cancer is a common malignancy and a major cause of death in women worldwide. The immunomodulatory role of B cells is being increasingly recognized in autoimmune diseases and cancers. In recent years, immunotherapeutic strategies that upregulate the patient's own antitumor T cell responses have shown promise in ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.10.013

    authors： Zhu S,Wang X,Wang J,Lin J,Cong Y,Qiao G

    更新日期：2017-12-01 00:00:00

  • Inducible endothelial cell-specific gene expression in transgenic mouse embryos and adult mice.

    abstract：:Utilizing both the TET-OFF and TET-ON systems in combination with transcriptional control elements of the Tie-2 gene, we have established a series of transgenic activator and responder mice for TET-regulated endothelial cell-specific transgene expression in double transgenic mouse embryos and in adult mice. TET-regula...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.12.026

    authors： Deutsch U,Schlaeger TM,Dehouck B,Döring A,Tauber S,Risau W,Engelhardt B

    更新日期：2008-04-01 00:00:00

  • Interleukin-2 induces a rapid increase in ornithine decarboxylase mRNA in a cloned murine T lymphocytic cell line.

    abstract：:We recently observed a 25-fold increase in the activity of ornithine decarboxylase (ODC) 6 h after treatment of G1-synchronized CTLL-2 cells with interleukin-2 (IL-2). Here we show that the ODC mRNA content increased in parallel with the ODC activity during the first hours of stimulation with IL-2, resulting in a 25-f...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90201-2

    authors： Legraverend C,Potter A,Hölttä E,Alitalo K,Andersson LC

    更新日期：1989-03-01 00:00:00

  • Effector mechanisms of fenretinide-induced apoptosis in neuroblastoma.

    abstract：:Fenretinide is an effective inducer of apoptosis in many malignancies but its precise mechanism(s) of action in the induction of apoptosis in neuroblastoma is unclear. To characterize fenretinide-induced apoptosis, neuroblastoma cell lines were treated with fenretinide and flow cytometry was used to measure apoptosis,...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4988

    authors： Lovat PE,Ranalli M,Annichiarrico-Petruzzelli M,Bernassola F,Piacentini M,Malcolm AJ,Pearson AD,Melino G,Redfern CP

    更新日期：2000-10-10 00:00:00

  • Cytoskeleton protein 4.1R suppresses murine keratinocyte cell hyperproliferation via activating the Akt/ERK pathway in an EGFR-dependent manner.

    abstract：:The correct functioning of epidermal growth factor receptor (EGFR), a receptor tyrosine kinase, is required for normal skin development and homeostasis. Cellular hyperproliferation induced by dysregulation of EGFR is tightly associated with structural and functional defects of hair follicles, skin lesions, and tumorig...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.111648

    authors： Chen L,Wang T,Ji X,Ding C,Liang T,Liu X,Lu J,Guo X,Kang Q,Ji Z

    更新日期：2019-11-15 00:00:00

  • Tumor-specific induction of apoptosis by a p53-reactivating compound.

    abstract：:The tumor suppressor function of p53 is disabled in the majority of tumors, either by a point mutation of the p53 gene, or via MDM2-dependent proteasomal degradation. We have screened a chemical library using a cell-based assay and identified a low molecular weight compound named MITA which induced wild-type p53-depen...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.11.009

    authors： Hedström E,Issaeva N,Enge M,Selivanova G

    更新日期：2009-02-01 00:00:00

  • Mitosis-arresting effect of the calcium channel inhibitor SK&F 96365 on human leukemia cells.

    abstract：:The effect of SK&F 96365 (1-(beta-[3-(4-methoxyphenyl)propoxyl]-4- methoxyphenethyl)-1H-imidazole hydrochloride), a recently synthesized inhibitor of receptor-mediated calcium entry, was investigated on human hematopoietic cell lines. We found that treatment of the T-cell leukemia line Jurkat with SK&F 96365 inhibited...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90103-f

    authors： Nordström T,Nevanlinna HA,Andersson LC

    更新日期：1992-10-01 00:00:00

  • Flow cytometric analysis of fluorescein-labeled nerve growth factor binding to A875 human melanoma cells.

    abstract：:The interaction of fluorescein-labeled nerve growth factor (NGF) with human melanoma cells (A875) has been studied in order to assess better methodology for rigorous NGF binding studies. The NGF was modified at a single carboxyl group with iodoacetamidofluorescein after reaction with carbodiimide and cystamine. The mo...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1012

    authors： Kasaian MT,Jacobberger JW,Neet KE

    更新日期：1994-01-01 00:00:00

  • A simple technique for quantitation of low levels of DNA damage in individual cells.

    abstract：:Human lymphocytes were either exposed to X-irradiation (25 to 200 rads) or treated with H2O2 (9.1 to 291 microM) at 4 degrees C and the extent of DNA migration was measured using a single-cell microgel electrophoresis technique under alkaline conditions. Both agents induced a significant increase in DNA migration, beg...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(88)90265-0

    authors： Singh NP,McCoy MT,Tice RR,Schneider EL

    更新日期：1988-03-01 00:00:00

  • Fibroblast contractile force is independent of the stiffness which resists the contraction.

    abstract：:Using a device named the cell force monitor, the contractile force developed by fibroblasts has been studied by measuring the macroscopic contraction of porous collagen-glycosaminoglycan (GAG) matrices over the first 24 h following cell attachment. In this paper, the effect of a variation in the stiffness that resists...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5408

    authors： Freyman TM,Yannas IV,Yokoo R,Gibson LJ

    更新日期：2002-01-15 00:00:00

  • RCAN1.4 acts as a suppressor of cancer progression and sunitinib resistance in clear cell renal cell carcinoma.

    abstract：:Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors in the urinary system, and its incidence continues to increase. Regulator of calcineurin 1 (RCAN1), one of the genes on chromosome 21, is a crucial mediator of tumor inhibition. RCAN1.4 is best characterized as an endogenous inhibitor o...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.09.017

    authors： Song Z,Cao Q,Ruan H,Yang H,Wang K,Bao L,Cheng G,Xu T,Xiao H,Wang C,Liu D,Chen K,Zhang X

    更新日期：2018-11-15 00:00:00

  • Low pH value of pericellular medium as a factor limiting cell proliferation in dense cultures.

    abstract：:An attempt was made to determine the role of metabolic acidification of pericellular medium in regulating cell proliferation. A method of measuring the pH of pericellular medium at a distance exceeding a Debye radius of 5-10 A from the cell surface (pHp) was developed. The values of pHp and pH measured in the medium a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90188-0

    authors： Akatov VS,Lezhnev EI,Vexler AM,Kublik LN

    更新日期：1985-10-01 00:00:00

  • The von Hippel-Lindau tumor suppressor protein influences microtubule dynamics at the cell periphery.

    abstract：:The von Hippel-Lindau (VHL) protein protects microtubules (MTs) from destabilization by nocodazole treatment. Based on this fixed-cell assay with static end points, VHL has been reported to directly stabilize the MT cytoskeleton. To investigate the dynamic changes in MTs induced by VHL in living cells, we measured the...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.07.016

    authors： Lolkema MP,Mehra N,Jorna AS,van Beest M,Giles RH,Voest EE

    更新日期：2004-12-10 00:00:00

  • Differential expression of Raf-1 protooncogene in resting and activated human leukocyte populations.

    abstract：:In this study we examined by Northern blot analysis the expression of Raf-1 protooncogene in normal human peripheral blood leukocytes. Unlike thymocytes, circulating lymphocytes did not express appreciable levels of Raf-1 mRNA. In contrast, polymorphonuclear cells (PMN) had high levels of Raf-1 transcripts. Also densi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90366-3

    authors： Colotta F,Polentarutti N,Mantovani A

    更新日期：1991-06-01 00:00:00

  • Defect in prereplicative phase of G0-specific ts mutant, tsJT60.

    abstract：:A temperature-sensitive mutant, tsJT60, grew exponentially at both 34 degrees and 39.5 degrees C, but when stimulated from the resting state it entered S phase at 34 degrees but not at 39.5 degrees C. The mutated function appeared to be a prerequisite throughout from 0 to 9 h following the stimulation, in order that G...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90543-4

    authors： Ninomiya-Tsuji J,Goto Y,Ishibashi S,Ide T

    更新日期：1986-07-01 00:00:00

  • Cellular and cytoskeleton morphology and strength of adhesion of cells on self-assembled monolayers of organosilanes.

    abstract：:The objective of this study was to explore the potential use of self-assembled monolayers (SAMs) of alkylamine and arylalkyamine as well-defined, homogeneous, tailored in vitro model surfaces for exploring the effect of hydrodynamic flow on morphology and strength of adhesion of human umbilical vein endothelial cells....

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4156

    authors： Kapur R,Rudolph AS

    更新日期：1998-10-10 00:00:00