Fibroblast contractile force is independent of the stiffness which resists the contraction.

Abstract:

:Using a device named the cell force monitor, the contractile force developed by fibroblasts has been studied by measuring the macroscopic contraction of porous collagen-glycosaminoglycan (GAG) matrices over the first 24 h following cell attachment. In this paper, the effect of a variation in the stiffness that resists matrix contraction by cells on the contractile force generated by the cells was determined. Data from these experiments revealed that the contractile force generated by the fibroblasts was independent of the stiffness of the resistance within the range tested (0.7-10.7 N/m). These results suggest that during the time when fibroblasts are attaching to and spreading on collagen-GAG matrices the contractile forces they generate are force limited, not displacement limited. Therefore, the cytoskeletal mechanism of force generation, corresponding with cell elongation, is capable of increasing the displacement of adhesion sites in order to develop the same level of force. Although a detailed understanding of how the passive mechanical signals provided by substrate materials affect cell processes is still unavailable, in vitro modeling of cell-mediated contraction continues to provide useful information.

journal_name

Exp Cell Res

authors

Freyman TM,Yannas IV,Yokoo R,Gibson LJ

doi

10.1006/excr.2001.5408

keywords:

subject

Has Abstract

pub_date

2002-01-15 00:00:00

pages

153-62

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014482701954084

journal_volume

272

pub_type

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