Abstract:
BACKGROUND:Capsular contracture is an incurable complication after silicone-based implant surgery. Myofibroblast is the predominant cell in the contracted capsule. We hypothesized that human adipose derive stromal cells (hASCs) together with fibroblast may show a similar phenotypic characteristics of myofibroblast after the treatment of inflammatory cytokines in vitro. MATERIALS AND METHODS:Interleukin 3 (IL-3) and granulocyte macrophage colony stimulating factor (GM-CSF) were treated in the culture of hASCs and HDFs. Lyn peptide inhibitor was applied as an inhibitor. The changes of cell surface markers (CD105, CD73, CD34, CD45, CD31, CD325 and CD146) were assessed. The expression of various cytokines related to wound contraction were tested such as TGF-β, α-SMA, HGF, FGF, ENT-1, and TSP-1. Myo-D, α-SMA, and glial fibrillary acidic protein (GFAP) were evaluated by blotting and immunocytochemical staining. The collagen-gel contraction assay was performed for the functional contraction of myofibroblastic phenotype. RESULTS:The expression of α-SMA, Myo-D and GFAP after the treatment of IL-3/GM-CSF showed similar results in hASCs and HDFs. Enhanced expression of TGF- β was observed in HDFs and the increase of ENT-1 and TSP-1 was significant in hASCs. Collagen-gel with HDFs contracted significantly within 24h after the treatment of IL-3/GM-CSF, and the contraction was inhibited by Lyn peptide inhibitor. But in hASCs, the gel-contraction was not significant. CONCLUSION:IL-3/ GM-CSF effected on the myofibroblastic differentiation of hASCs as well as it did on HDFs. But hASCs did not show the phenotypic gel-contraction within 24h.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Lee JS,Tae SS,Kim DY,Han SK,Kim WK,Dhong ESdoi
10.1016/j.yexcr.2017.03.056subject
Has Abstractpub_date
2017-06-15 00:00:00pages
67-82issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(17)30185-4journal_volume
355pub_type
杂志文章abstract::Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population and show significant expansion under pathological conditions. microRNA plays important roles in many biological processes, whether microRNAs have a function in the expansion of MDSCs is still not very clear. In this study, miR-34a overexpression c...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.04.010
更新日期:2014-08-15 00:00:00
abstract::Histidine-rich glycoprotein (HRGP) is an abundant heparin-binding plasma protein. We have shown that a fragment released from the central histidine/proline-rich (His/Pro-rich) domain of HRGP blocks endothelial cell migration in vitro and vascularization and growth of murine fibrosarcoma in vivo. The minimal active HRG...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.04.022
更新日期:2006-08-01 00:00:00
abstract::Acetylcholine receptors (AChRs) of rat muscle cells grown in culture for 4 days were labeled with 125I-alpha-bungarotoxin and their degradation rate measured. Two AChR populations, a rapidly degrading one (Rr,t1/2 approximately 1 day) and a slowly degrading one (Rs, t1/2 approximately 4 days) were identified at a rati...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1220
更新日期:1993-09-01 00:00:00
abstract::Cell migration plays a major role during wound healing and is tightly controlled by a variety of growth factors and extracellular matrix proteins. The experiments reported here have been designed to study whether defined beta 1 integrins are involved in the platelet-derived growth-factor-AB (PDGF-AB)-modulated migrato...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1288
更新日期:1995-09-01 00:00:00
abstract::Lysosomal cysteine proteinase cathepsin B is implicated in remodeling the extracellular matrix, a crucial step in the process of tumor cell invasion. In this study the contributions of intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT,...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(02)00055-1
更新日期:2003-02-15 00:00:00
abstract::Extraocular muscles (EOMs) are categorized as skeletal muscles; however, emerging evidence indicates that their gene expression profile, metabolic characteristics and functional properties are significantly different from the prototypical members of this muscle class. Gene expression profiling of developing and adult ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2010.03.018
更新日期:2010-07-15 00:00:00
abstract:BACKGROUND:Müller cell gliosis not only plays an important physiological role by maintaining retinal neuronal homeostasis but is also associated with multiple pathological events in the retina, including optic nerve crush (ONC) injury. Modulating Müller cell gliosis contributes to the creation of a permissive environme...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2019.01.009
更新日期:2019-02-15 00:00:00
abstract::Retromer is a multimeric protein complex that mediates intracellular receptor sorting. One of the roles of retromer is to promote transcytosis of the polymeric immunoglobulin receptor (pIgR) and its ligand polymeric immunoglobulin A (pIgA) in polarized epithelial cells. In Madin-Darby Canine Kidney (MDCK) cells, overe...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.11.010
更新日期:2007-02-15 00:00:00
abstract::Tabby and downless mutant mice have identical phenotypes characterized by deficient development of several ectodermally derived organs such as teeth, hair, and sweat glands. Edar, encoded by the mouse downless gene and defective in human dominant and recessive forms of autosomal hypohidrotic ectodermal dysplasia (EDA)...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5331
更新日期:2001-10-01 00:00:00
abstract::Ran, a nuclear GTPase, and a number of interacting proteins, including regulators RanGEF1 and RanGAP1, are involved in nucleocytoplasmic transport. We have identified a new temperature-sensitive mutation in budding yeast YRB1 gene, which encodes Ran-binding protein-1 (RanBP1). In contrast to other yrb1 alleles, the ne...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4174
更新日期:1998-10-10 00:00:00
abstract::The effects of the tubulin-binding drug colchicine on cultured neonate cardiac cell function were investigated. Application of low doses of colchicine (but not lumicolchicine) caused an early reversible increase in beating rate with a concomitant decrease in amplitude. Treatment of the cells with trypsin at a dose tha...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90044-3
更新日期:1986-06-01 00:00:00
abstract::Estrogen receptor-containing cells were identified in uteri of mature and senescent rats by means of quantitative autoradiography. Cell numbers and density were assessed in the various uterine layers. It was determined that despite decreases in receptor concentrations in endometrium, endometrial stroma, myometrium, an...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90227-9
更新日期:1989-01-01 00:00:00
abstract::Growth factor-mediated stimulation of epithelial cells induces the disassembly of E-cadherin-mediated cell-cell adhesion. We found that overexpression of a disintegrin and metalloprotease 9 (ADAM9) enhanced growth factor-mediated induction of endocytosis and dynamic recycling of E-cadherin in HT29 human colon cancer c...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.10.032
更新日期:2006-02-01 00:00:00
abstract::Nucleoporins (Nups) are a large and diverse family of proteins that mediate nucleocytoplasmic transport at interphase of vertebrate cells. Nups also function in mitosis progression. However, whether Nups are involved in oocyte meiosis progression is still rarely known. In this study, we delineated the roles and regula...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.09.004
更新日期:2018-10-15 00:00:00
abstract::Several metabolic enzymes assemble into distinct intracellular structures in prokaryotes and eukaryotes suggesting an important functional role in cell physiology. The CTP-generating enzyme CTP synthase forms long filamentous structures termed cytoophidia in bacteria, yeast, fruit flies and human cells independent of ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.03.042
更新日期:2017-05-15 00:00:00
abstract::Phosphorylation of the nucleosome core histone H3 (H3) on Ser-10 is thought to be a prerequisite for chromatin condensation at mitosis. Although during interphase, cell differentiation, or mitogenic activation of quiescent cells, changes in chromatin structure that involve local chromatin condensation/decondensation a...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4283
更新日期:1999-01-10 00:00:00
abstract::By fluorescence spectroscopy, the average pH within endocytic compartments was determined during endocytosis of fluorescein conjugates by macrophages and hepatocytes. In mouse macrophages and hepatocytes fluorescein conjugates taken up either in the fluid phase or by binding to cell surface receptors were rapidly tran...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90699-2
更新日期:1984-01-01 00:00:00
abstract::MicroRNAs (miRNAs) are small, non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. In the brain, a large number of miRNAs are expressed and there is a growing body of evidence demonstrating that miRNAs are essential for brain development and neuronal function. Conditional knockou...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2013.09.022
更新日期:2014-02-01 00:00:00
abstract::Experiments are described which show that the torsional oscillations arising in a strand of the slime mould, Physarum polycephalum, under certain conditions remain closely correlated to oscillations in cytoplasmic streaming. When this is the case, the direction of rotation is related to the direction of streaming by a...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(83)90401-9
更新日期:1983-11-01 00:00:00
abstract::Viral vectors hold promise and challenges in gene therapy. Specifically, we have previously shown that baculoviral (BV) vectors have a high efficiency of gene delivery in human embryonic stem (ES) cells. Here we report the development of a complementary system to further our evaluation by utilizing the laboratory fish...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.04.015
更新日期:2009-08-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) hold tremendous potential for the development of new regenerative medicine therapies and the study of molecular mechanisms of pluripotency and development. However, reactivation of c-Myc, which results in tumor formation in chimeric mice, is a major roadblock in the translation o...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2013.09.014
更新日期:2013-12-10 00:00:00
abstract::During skeletal muscle development three intermediate filament proteins are expressed: nestin, vimentin, and desmin. Vimentin and desmin belong to the class III intermediate filaments and are closely related to each other, whereas nestin is a more distantly related, class VI, intermediate filament. It was previously o...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1281
更新日期:1994-10-01 00:00:00
abstract::Vegetative amoebae of Dictyostelium discoideum (strain AX2) adhere to columns of hydrophobic beads (Bio-Beads SM-2) which have been coated with axenic growth medium. This binding is blocked by sodium azide and other inhibitors of aerobic respiration. Bound cells are not detached by lowering the temperature but they ca...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90235-0
更新日期:1984-05-01 00:00:00
abstract::The role of glycans in the apical targeting of proteins in epithelial cells remains a debated question. We have expressed the mouse soluble dipeptidyl peptidase IV (DPP IV ectodomain) in kidney (MDCK) and in intestinal (Caco-2) epithelial cell lines, as a model to study the role of glycosylation in apical targeting. T...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.4894
更新日期:2000-07-10 00:00:00
abstract::Apoptosis is characterized by typical morphological changes and most frequently fragmentation of DNA into oligonucleosome-size fragments. In order to investigate whether an alteration in the mechanisms involved in the process of apoptosis could contribute to cellular resistance, induction of apoptosis was studied in a...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1148
更新日期:1995-05-01 00:00:00
abstract::In multiple myeloma, which commonly depends on interleukin 6, IL-6, survival signaling induced by this cytokine is largely mediated by activation of STAT3. Interferon alpha (IFNalpha) treatment of cell lines derived from multiple myeloma or of myeloma tumor cells ex vivo leads to apoptosis. In this study we demonstrat...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.08.007
更新日期:2007-11-15 00:00:00
abstract::Phosphatidylinositol 3-kinase inhibitors have been shown to affect endocytosis or subsequent intracellular sorting in various receptor systems. Agonist-activated beta(2)-adrenergic receptors undergo desensitization by mechanisms that include the phosphorylation, endocytosis and degradation of receptors. Following endo...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.04.034
更新日期:2007-07-15 00:00:00
abstract::The sodium-coupled bicarbonate cotransporter 1 (NBCe1) plays an essential role in the maintenance of acid-base homeostasis in the human body. However, little research has been done regarding the modification of NBCe1. Nedd4-2 is one of the most important ubiquitin E3 ligases in the kidney where it is responsible for m...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2020.111958
更新日期:2020-05-15 00:00:00
abstract::Inhibition of cAMP-dependent protein kinase activity by microinjection of a specific physiologic protein inhibitor into sea urchin eggs inhibits the first cleavage after fertilization. Inhibition apparently occurs at some time prior to or during formation of the mitotic spindle. Measurement of the total protein kinase...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90286-j
更新日期:1990-05-01 00:00:00
abstract::The gene cluster for human U2 RNA has been mapped to chromosome 17q21 by in situ hybridization and hybridization analysis of DNA from mouse/human somatic cell hybrids. ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(85)80020-3
更新日期:1985-08-01 00:00:00