Abstract:
:Cell migration plays a major role during wound healing and is tightly controlled by a variety of growth factors and extracellular matrix proteins. The experiments reported here have been designed to study whether defined beta 1 integrins are involved in the platelet-derived growth-factor-AB (PDGF-AB)-modulated migratory response to collagen type I and to fibronectin. Preincubation of fibroblasts with PDGF-AB resulted in an up to 2.5-fold increase in the migratory response to collagen type I as well as fibronectin and to enhanced synthesis and cell surface expression of the alpha 2, alpha 3, alpha 5, and beta 1 integrin subunits. Function-blocking monoclonal antibodies against the common beta 1 integrin subunit dose-dependently inhibited the PDGF-AB-augmented migration of fibroblasts to collagen type I and fibronectin. The PDGF-AB-induced migration to collagen type I was also inhibited by antibodies against the alpha 2 integrin subunit, whereas the corresponding migration to fibronectin was almost completely blocked by the combined application of antibodies against the alpha 3 and the alpha 5 integrin subunits. Taken together, up-regulation of integrin synthesis and expression by human recombinant PDGF-AB correlate with an increase in the migratory response of dermal human fibroblasts to various extracellular matrix proteins and thus may contribute to an efficient regulation of cell migration during wound healing and tissue repair.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Kirchberg K,Lange TS,Klein EC,Jungtäubl H,Heinen G,Meyer-Ingold W,Scharffetter-Kochanek Kdoi
10.1006/excr.1995.1288subject
Has Abstractpub_date
1995-09-01 00:00:00pages
29-35issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(85)71288-8journal_volume
220pub_type
杂志文章abstract::There is at present a controversy in the literature whether MSCs are susceptible to spontaneous in vitro transformation or not. Several groups have reported spontaneous transformation of MSCs from various species. However, some of these reports were not true transformations and later proven to be due to cross-contamin...
journal_title:Experimental cell research
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pub_type: 杂志文章
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更新日期:1990-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-08-25 00:00:00
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更新日期:1996-06-15 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.02.015
更新日期:2009-07-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1296
更新日期:1993-11-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1362
更新日期:1995-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.yexcr.2013.05.008
更新日期:2013-07-15 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.05.006
更新日期:2007-07-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0071
更新日期:1996-02-25 00:00:00
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pub_type: 杂志文章
doi:10.1016/0014-4827(78)90382-8
更新日期:1978-05-01 00:00:00