Abstract：
:The tyrosine phosphatase PTP1B is an important regulator of cell function. In living cells PTP1B activity is restricted to the vicinity of the endoplasmic reticulum (ER) by post-translational C-terminal attachment of PTP1B to the ER membrane network. In our study we investigated the membrane anchor of PTP1B by use of EGFP fusion proteins. We demonstrate that the membrane anchor of PTP1B cannot be narrowed down to a unique amino acid sequence with a defined start and stop point but rather is moveable within several amino acids. Removal of up to seven amino acids from the C-terminus, as well as exchange of single amino acids in the putative transmembrane sequence did not influence subcellular localization of PTP1B. With the method of bimolecular fluorescence complementation we could demonstrate dimerization of PTP1B in vivo. Homodimerization was, in contrast to other tail-anchored proteins, not dependent on the membrane anchor. Our data demonstrate that the C-terminal membrane anchor of PTP1B is formed by a combination of a single stretch transmembrane domain (TMD) followed by a tail. TMD and tail length are variable and there are no sequence-specific features. Our data for PTP1B are consistent with a concept that explains the ER membrane anchor of tail-anchored proteins as a physicochemical structure.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Anderie I,Schulz I,Schmid Adoi
10.1016/j.yexcr.2007.05.025subject
Has Abstractpub_date
2007-09-10 00:00:00pages
3189-97issue
15eissn
0014-4827issn
1090-2422pii
S0014-4827(07)00286-8journal_volume
313pub_type
杂志文章abstract：:As chemical reactions related to the regulation of cell proliferation are governed by availability, amount, and concentration of relevant molecules, it has been suggested that cell size is an important factor in the control of cell cycle. We have measured the size of proliferating cells of Allium cepa roots in which g...
journal_title：Experimental cell research
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abstract：BACKGROUND:Emerging studies demonstrate that long noncoding RNAs (lncRNAs) play crucial roles in hepatocarcinogenesis through various mechanisms. LncRNA CCAT2 was a newly discovered lncRNA and amplified in several cancers. However, the mechanisms involved in function of CCAT2 in hepatocellular carcinoma (HCC) remain to...
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2007.12.001
更新日期：2008-03-10 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5408
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abstract：:Paclitaxel (Taxol) has been successfully combined with the monoclonal antibody trastuzumab (Herceptin) in the treatment of ErbB2 overexpressing cancers. However, this combination therapy showed an unexpected synergistic increase in cardiac dysfunction. We have studied the mechanisms of paclitaxel/anti-ErbB2 cardiotoxi...
journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2007.02.007
更新日期：2007-05-01 00:00:00
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pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.12.003
更新日期：2013-02-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.04.013
更新日期：2012-08-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90130-1
更新日期：1983-07-01 00:00:00
abstract：:Mouse embryonic stem (ES) cells are isolated from the inner cell mass (ICM)/epiblast of preimplantation embryos and are widely used in cell differentiation studies. We have previously observed differences in transcript and antigen expression following differentiation of ES cells lines in vitro. We have investigated th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2003.10.017
更新日期：2004-02-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90522-v
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90280-n
更新日期：1990-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.12.003
更新日期：2018-01-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2014.07.016
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(82)90408-6
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0143
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doi：10.1016/s0014-4827(02)00055-1
更新日期：2003-02-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90371-9
更新日期：1984-03-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.03.017
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.11.021
更新日期：2009-04-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.10.019
更新日期：2010-02-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2004.02.032
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.10.037
更新日期：2009-02-01 00:00:00
abstract：:Tetracyclines have long been used as valuable broad-spectrum antibiotics. The high antibacterial activity of tetracyclines, combined with their good tolerability, has led to their widespread use in treating various infectious diseases. However, similar to other antibiotics, tetracyclines are also known for their adver...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.11.012
更新日期：2014-01-15 00:00:00
abstract：:We have studied the mechanisms of angiotensin II (A-II) transport through a cultured arterial endothelial cell monolayer. The transport of 125I-labeled A-II was inhibited by excess unlabeled A-II (50 microM) and [Sar1, Ile8]-A-II (50 microM), but was not inhibited by bradykinin (50 microM). The transport process was s...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90149-5
更新日期：1990-09-01 00:00:00
