Abstract:
:Growth factor-mediated stimulation of epithelial cells induces the disassembly of E-cadherin-mediated cell-cell adhesion. We found that overexpression of a disintegrin and metalloprotease 9 (ADAM9) enhanced growth factor-mediated induction of endocytosis and dynamic recycling of E-cadherin in HT29 human colon cancer cells. In addition, ubiquitination and degradation of E-cadherin were reduced in these cells. ADAM9 constitutively interacted with E-cadherin, and the two proteins co-localized at the plasma membrane of HT29 cells. Administration of a metalloprotease inhibitor or overexpression of an ADAM9 mutant lacking metalloprotease activity attenuated growth factor-dependent endocytosis and recycling of E-cadherin as well as scattering of HT29 cells. These results suggest that the metalloprotease activity of ADAM9 mediates growth factor-induced endocytosis and dynamic recycling of E-cadherin and prevents E-cadherin degradation.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Hirao T,Nanba D,Tanaka M,Ishiguro H,Kinugasa Y,Doki Y,Yano M,Matsuura N,Monden M,Higashiyama Sdoi
10.1016/j.yexcr.2005.10.032keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
331-9issue
3eissn
0014-4827issn
1090-2422pii
S0014-4827(05)00508-2journal_volume
312pub_type
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journal_title:Experimental cell research
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