Abstract：
:Retinol binding protein (RBP) is the plasma transport protein of retinol. Mobilization of RBP from the liver stores is stimulated by retinol. During vitamin A deficiency, RBP secretion is specifically inhibited while its rate of biosynthesis is unaffected. As a consequence, RBP, as apoprotein, accumulates inside the endoplasmic reticulum (ER) of the hepatocyte, and a new elevated steady-state concentration is reached. We have studied the role of degradation on the regulation of RBP metabolism in retinol deficient HepG2 cells and determined the intracellular site where RBP degradation takes place. Pulse-chase experiments show that RBP half-life is ca.9 h in retinol-depleted cells. RBP degradation is slow and is insensitive to the treatment with NH4Cl, which inactivates lysosomal proteases and to the drug brefeldin A, which prevents protein export from the ER. The data obtained suggest that RBP degradation occurs, at least in part, in a pre-Golgi compartment. 2-Mercaptoethanol, at millimolar concentration, induces RBP secretion, suggesting a possible role for sulfhydryl-mediated apo-RBP retention by resident ER proteins.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Tosetti F,Ferrari N,Pfeffer U,Brigati C,Vidali Gdoi
10.1016/0014-4827(92)90197-gkeywords:
subject
Has Abstractpub_date
1992-06-01 00:00:00pages
467-72issue
2eissn
0014-4827issn
1090-2422journal_volume
200pub_type
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journal_title：Experimental cell research
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更新日期：1998-03-15 00:00:00
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更新日期：1994-03-01 00:00:00
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更新日期：1991-03-01 00:00:00
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更新日期：2019-09-15 00:00:00
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更新日期：1997-11-25 00:00:00
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更新日期：2017-10-01 00:00:00
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doi：10.1006/excr.1995.1070
更新日期：1995-03-01 00:00:00
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更新日期：1989-07-01 00:00:00
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更新日期：2017-02-01 00:00:00
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更新日期：1985-08-01 00:00:00
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更新日期：1997-12-15 00:00:00
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pub_type： 杂志文章
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更新日期：2004-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1997-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0014-4827(91)90179-x
更新日期：1991-01-01 00:00:00
