Abstract：
:An epithelial intestinal cell line has been established from explants of fetal rat small intestine. After the 9th passage (approx. 25 population doublings) epithelial-like cells acquired the properties of a permanent cell line. The epithelial nature of this cell line, and of clone IRD 98 subsequently isolated, is supported by morphological and ultrastructural criteria, and also by the presence of enzymes characteristic of enterocytes, such as aminopeptidase, alkaline phosphatase, gamma-glutamyl transferase, lactase and maltase. The occurrence of the triglyceride pathway enzyme monoacylglycerol acyltransferase and of apoproteins (Apo A1 and Apo E) can also be demonstrated. Taken together, the results presented here provide evidence that clone IRD 98 is an epithelial cell line, most likely originating from the relatively differentiated cell layer of fetal rat small intestine.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Négrel R,Rampal P,Nano JL,Cavenel C,Ailhaud Gdoi
10.1016/0014-4827(83)90069-1subject
Has Abstractpub_date
1983-02-01 00:00:00pages
427-37issue
2eissn
0014-4827issn
1090-2422journal_volume
143pub_type
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