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Growth stimulation of rat primary embryo fibroblasts by the human myc gene.

Abstract：

:We have studied the ability of plasmids encoding a normal human myc protein to stimulate growth of primary rat embryo fibroblasts. We measured growth stimulation by the number of G418-resistant colonies obtained after co-transfection with plasmid pSV2neo and by the percentage of these colonies that grew in long-term culture (immortalization). Using a normal human myc gene, we detected a weak growth stimulation at the colony formation stage and a low frequency of immortalization. Replacement of the myc promoter by a heterologous promoter (mouse metallothionein I promoter) and deletion of the first non-coding exon led to a more efficient growth stimulation by both criteria. Thus, disregulation of c-myc is essential for an altered pattern of growth. Using zinc, a metallothionein inducer, we observed a slight increase in the growth rate of some transfectants, which can be measured by thymidine incorporation. However, the relative inefficiency of immortalization we observed suggests that either a high level of myc expression or participation of other genes is required for establishment in culture. Under our experimental conditions, we could not detect a transforming activity for the human myc gene and none of our myc-containing cell lines was tumorigenic in nude mice.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Nicolaiew N,Dautry F

doi

10.1016/0014-4827(86)90482-9

subject

Has Abstract

pub_date

1986-10-01 00:00:00

pages

357-69

issue

2

eissn

0014-4827

issn

1090-2422

pii

0014-4827(86)90482-9

journal_volume

166

pub_type

杂志文章

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