Cytochemical localization of adenylate cyclase in bovine cumulus-oocyte complexes.

abstract：:We report that prosaposin binds to U937 and is active as a protective factor on tumor necrosis factor alpha (TNFalpha)-induced cell death. The prosaposin-derived saposin C binds to U937 cells in a concentration-dependent manner, suggesting that prosaposin behaves similarly. Prosaposin binding induces U937 cell death p...

journal_title：Experimental cell research

authors： Misasi R,Garofalo T,Di Marzio L,Mattei V,Gizzi C,Hiraiwa M,Pavan A,Grazia Cifone M,Sorice M

更新日期：2004-08-01 00:00:00

abstract：:A monoclonal antibody raised against microtubule-associated protein-1 (MAP-1) binds to nuclei of normal human fibroblasts, its binding site being detected as intranuclear immunofluorescent dots. The percentage of cells showing these dots increased with insulin, hydrocortisone, epidermal growth factor (EGF), and transf...

journal_title：Experimental cell research

authors： Ohno T,Ohkawa A,Sato C

更新日期：1985-06-01 00:00:00

abstract：:The objective of the present study was to develop an accurate and reproducible method for liver sinusoidal endothelial cell (LSEC) isolation in mice. Non-parenchymal cells were isolated using a modified two-step collagenase digestion combined with Optiprep density gradient centrifugation. LSEC were further purified us...

journal_title：Experimental cell research

authors： Meyer J,Lacotte S,Morel P,Gonelle-Gispert C,Bühler L

更新日期：2016-12-10 00:00:00

abstract：:LC3, a mammalian homologue of yeast Atg8, is assumed to play an important part in bulk sequestration and degradation of cytoplasm (macroautophagy), and is widely used as an indicator of this process. To critically examine its role, we followed the autophagic flux of LC3 in rat hepatocytes during conditions of maximal ...

journal_title：Experimental cell research

authors： Szalai P,Hagen LK,Sætre F,Luhr M,Sponheim M,Øverbye A,Mills IG,Seglen PO,Engedal N

更新日期：2015-04-10 00:00:00

abstract：:We found that a transient rise in cGMP levels, which was closely associated with the Ca2+ influx, occurred concomitant with the onset of myoblast fusion. The Ca2+ channel blocker D600 decreased both the cell fusion and the normal rise in cGMP levels. In contrast, the Ca2+ ionophore A23187 transiently increased cGMP le...

journal_title：Experimental cell research

authors： Choi SW,Baek MY,Kang MS

更新日期：1992-03-01 00:00:00

abstract：:It has previously been shown by immunocytochemistry that the inactive X chromosome (Xi) in somatic cells of human and mouse females is marked by underacetylation of histone H4. It has been suggested that this may be important for transcriptional silencing of genes on Xi. We have now investigated X-inactivation in meio...

journal_title：Experimental cell research

authors： Armstrong SJ,Hultén MA,Keohane AM,Turner BM

更新日期：1997-02-01 00:00:00

abstract：:Different extracellular matrix (ECM) molecules, when presented to hippocampal neurons in culture in a substrate-bound form, exert strikingly similar effects on the establishment of neuronal polarity, i.e., the growth of axon-like major neurites is favored, whereas extension of dendrite-like minor neurites is inhibited...

journal_title：Experimental cell research

authors： Lochter A,Schachner M

更新日期：1997-08-25 00:00:00

abstract：:Barrier-to-autointegration factor (BAF) is an essential chromatin protein conserved in metazoans. BAF has roles in nuclear assembly, chromatin organization, gene expression, and gonad development and is exploited by retroviruses. BAF forms stable dimers that bind nonspecifically to dsDNA and specifically to LEM-domain...

journal_title：Experimental cell research

authors： Tifft KE,Segura-Totten M,Lee KK,Wilson KL

更新日期：2006-02-15 00:00:00

abstract：:The retinoblastoma tumor suppressor protein (RB) is activated/dephosphorylated to mediate cell cycle inhibition in response to antimitogenic signals. To elucidate the mode of RB action at this critical transition, we utilized cell lines that can be induced to express a constitutively active allele of RB (PSM-RB). As e...

journal_title：Experimental cell research

authors： Angus SP,Fribourg AF,Markey MP,Williams SL,Horn HF,DeGregori J,Kowalik TF,Fukasawa K,Knudsen ES

更新日期：2002-06-10 00:00:00

abstract：:Apoptosis is characterized by typical morphological changes and most frequently fragmentation of DNA into oligonucleosome-size fragments. In order to investigate whether an alteration in the mechanisms involved in the process of apoptosis could contribute to cellular resistance, induction of apoptosis was studied in a...

journal_title：Experimental cell research

authors： Segal-Bendirdjian E,Jacquemin-Sablon A

更新日期：1995-05-01 00:00:00

abstract：:In studies of the ontogeny of fibroblast-epithelial interactions during late fetal lung rat lung development, we have identified two subpopulations of fibroblasts which differed in their ability to promote epithelial cell proliferation or differentiation. As glycosaminoglycans (GAGs) have been implicated in the regula...

journal_title：Experimental cell research

authors： Caniggia I,Tanswell K,Post M

更新日期：1992-10-01 00:00:00

abstract：:Blood vessels are one of the major routes for the dissemination of cancer cells. Malignant tumors release growth factors such as vascular endothelial growth factor(VEGF) to induce angiogenesis, thereby promoting metastasis. Here, we report that The Drosophila Eyes Absent Homologue 1 (EYA1), which is overexpressed in c...

journal_title：Experimental cell research

authors： Cai S,Cheng X,Liu Y,Lin Z,Zeng W,Yang C,Liu L,Chukwuebuka OA,Li W

更新日期：2018-06-01 00:00:00

abstract：:Spermatogonial stem cells (SSCs) support life-long spermatogenesis by self-renewing and producing spermatogonia committed to differentiation. In vitro, SSCs form three-dimensional spermatogonial aggregates (clusters) when cultured with glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (...

journal_title：Experimental cell research

authors： Ebata KT,Yeh JR,Zhang X,Nagano MC

更新日期：2011-06-10 00:00:00

abstract：:Vero cells were infected with the ts-045 strain of vesicular stomatitis virus, and the cells were incubated at 39 degrees C to accumulate the mutant G glycoprotein in the ER as a misfolded aggregate. Cycloheximide was added to the culture medium 3.5 h after infection to prevent further protein synthesis, and the tempe...

journal_title：Experimental cell research

authors： Lotti LV,Torrisi MR,Erra MC,Bonatti S

更新日期：1996-09-15 00:00:00

abstract：:Compact mouse morulae were decompacted in calcium-free medium and allowed to recompact in standard embryo culture medium. When the recompaction medium contained trifluoperazine (TFP)(0.05 mM), an inhibitor of the calcium-dependent protein calmodulin, the embryos failed to recompact. This effect could not be overcome b...

journal_title：Experimental cell research

authors： Bilozur M,Powers RD

更新日期：1982-11-01 00:00:00

abstract：:Pathological cardiac hypertrophy is a maladaptive response in a variety of organic heart disease (OHD), which is characterized by mitochondrial dysfunction that results from disturbed energy metabolism. SIRT3, a mitochondria-localized sirtuin, regulates global mitochondrial lysine acetylation and preserves mitochondri...

journal_title：Experimental cell research

authors： Yue Z,Ma Y,You J,Li Z,Ding Y,He P,Lu X,Jiang J,Chen S,Liu P

更新日期：2016-10-01 00:00:00

abstract：:The double-stranded RNA-dependent kinase, PKR, is encoded by an interferon inducible gene and is largely responsible for the anti-viral effects of this cytokine. Recent studies have shown that PKR may also play a role in the regulation of normal cellular growth. Although numerous examples of viral strategies for inact...

journal_title：Experimental cell research

authors： Abraham N,Jaramillo ML,Duncan PI,Méthot N,Icely PL,Stojdl DF,Barber GN,Bell JC

更新日期：1998-11-01 00:00:00

abstract：:Mitotic BHK21 cells were examined by immunofluorescence optical sectioning after staining with anti-vimentin. Throughout all stages of mitosis, intact 10-nm filaments were observed to form a cage around the mitotic spindle. These observations were confirmed by serial section electron microscopy which demonstrated asse...

journal_title：Experimental cell research

authors： Blose SH,Bushnell A

更新日期：1982-11-01 00:00:00

abstract：:Reduction of intercellular spaces in the areas of prospective cartilage and bone formation (precartilage condensation) precedes chondrogenesis and may represent an important step in the process of cartilage differentiation during limb skeletogenesis. We have attempted to clarify the role of the microenvironment establ...

journal_title：Experimental cell research

authors： Tacchetti C,Tavella S,Dozin B,Quarto R,Robino G,Cancedda R

更新日期：1992-05-01 00:00:00

abstract：:Cells undergoing apoptosis are recognized and rapidly phagocytosed by macrophages before their degradation, thus preventing the inflammatory reaction and protecting tissues from the damaging effects of released potentially harmful intracellular contents. In spite of growing interest in the mechanisms leading to the en...

journal_title：Experimental cell research

authors： Falasca L,Bergamini A,Serafino A,Balabaud C,Dini L

更新日期：1996-04-10 00:00:00

abstract：:Relay of information from the extracellular environment into the cell often results from a peptide growth factor binding to its cognate cell surface receptor; this event is an integral mechanism by which many cellular functions occur, including cell growth, motility, and survival. In recent years, however, this requir...

journal_title：Experimental cell research

authors： Boerner JL,Danielsen A,Maihle NJ

更新日期：2003-03-10 00:00:00

abstract：:Two genes, KIN1 and KIN2, were isolated in Saccharomyces cerevisiae by their homology to the protein kinase family of viral oncogenes. In previous studies, we identified the yeast KIN1 gene product (pp145KIN1) and KIN2 gene product (pp145KIN2) as 145 kDa phosphoproteins with serine/threonine protein kinase activity. W...

journal_title：Experimental cell research

authors： Tibbetts M,Donovan M,Roe S,Stiltner AM,Hammond CI

更新日期：1994-07-01 00:00:00

abstract：:Several metabolic enzymes assemble into distinct intracellular structures in prokaryotes and eukaryotes suggesting an important functional role in cell physiology. The CTP-generating enzyme CTP synthase forms long filamentous structures termed cytoophidia in bacteria, yeast, fruit flies and human cells independent of ...

journal_title：Experimental cell research

authors： Huang Y,Wang JJ,Ghosh S,Liu JL

更新日期：2017-05-15 00:00:00

abstract：:Coiled bodies (CBs) are structural constituents observed in nuclei of most eukaryotic cells. They usually occur in the nucleoplasm as well as in contact with the nucleolar surface. In this work we studied the hepatocyte nuclei of hibernating dormice in order to investigate possible modifications of CBs along the seaso...

journal_title：Experimental cell research

authors： Malatesta M,Zancanaro C,Martin TE,Chan EK,Amalric F,Lührmann R,Vogel P,Fakan S

更新日期：1994-04-01 00:00:00

abstract：:We used a glioblastoma multiform (GBM) cell line to study the mechanism of cellular regulation of nitric oxide (NO) production. Our experiments indicate a confluent monolayer of GBM cells to release NO as measured through its oxidized NO2 form which gradually accumulates and reaches a peak by 7 to 10 days of culture. ...

journal_title：Experimental cell research

authors： Haregewoin A,Alexander E 3rd,Black PM,Loeffler JS

更新日期：1994-01-01 00:00:00

abstract：:The subcellular location of a soluble DNA replication-stimulating factor released from lysolecithin-permeabilized cultured mammalian cells was investigated by cytochalasin B (CB) enucleation. In both Chinese hamster ovary cells and baby hamster kidney (BHK) cells, approx. 70% of the total activity of the replication-s...

journal_title：Experimental cell research

authors： Myers CA,Patel PI,Miller MR

更新日期：1983-01-01 00:00:00

abstract：:The ccl mutation in Paramecium tetraurelia reversibly and rapidly blocks cell cycle progression and DNA synthesis at the restrictive temperature. Progression through the cell cycle is blocked during both the G1 and S portions of the cell cycle, while at the restrictive temperature there is neither residual cell cycle ...

journal_title：Experimental cell research

authors： Rasmussen CD,Berger JD

更新日期：1984-12-01 00:00:00

abstract：:Advanced stage neuroblastomas (NB) exhibit a tissue inhibitor of metalloproteinase (TIMP)-2/matrix metalloproteinase (MMP) imbalance, considered a prerequisite for MMP involvement in tumor progression in vivo. Human SH-SY5Y NB cells exhibit a similar TIMP-2/MMP imbalance that promotes in vitro invasive behavior that i...

journal_title：Experimental cell research

authors： Cappabianca L,Farina AR,Tacconelli A,Mantovani R,Gulino A,Mackay AR

更新日期：2003-06-10 00:00:00

abstract：:In the fungus Achlya, sexual development in the male strain E87 is mediated by the steroid hormone antheridiol. Treatment of vegetatively growing cells of E87 with antheridiol resulted in changes in the [35S]methionine labeling of several secreted proteins. The most heavily labeled group of proteins observed in the se...

journal_title：Experimental cell research

authors： Brunt SA,Silver JC

更新日期：1986-03-01 00:00:00

abstract：:A prominent secretory glycoprotein was detected in the culture medium of porcine thyrocytes which was identified as clusterin by microsequencing. Treatment of thyrocytes with thyroid stimulating hormone revealed a tight regulation of both synthesis and secretion of clusterin, with a distinct fraction of clusterin bein...

journal_title：Experimental cell research

authors： Lemansky P,Brix K,Herzog V

更新日期：1999-08-25 00:00:00