当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Subcellular location of a soluble factor that stimulates DNA replication in permeable animal cells.

Subcellular location of a soluble factor that stimulates DNA replication in permeable animal cells.

Abstract：

:The subcellular location of a soluble DNA replication-stimulating factor released from lysolecithin-permeabilized cultured mammalian cells was investigated by cytochalasin B (CB) enucleation. In both Chinese hamster ovary cells and baby hamster kidney (BHK) cells, approx. 70% of the total activity of the replication-stimulating factor was associated with the karyoplast fraction. In neither cell line did the replication-stimulating factor show any significant change in activity or in subcellular location between G1 and S period synchronized cells. The general nature and mechanism of action of this stimulatory factor were characterized in order to show that it was similar to "cytoplasmic" factors reported to stimulate DNA replication in other subcellular systems. The predominantly nuclear location of the replication-stimulating factor supports a physiological role in DNA metabolism in animal cells.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Myers CA,Patel PI,Miller MR

doi

10.1016/0014-4827(83)90123-4

subject

Has Abstract

pub_date

1983-01-01 00:00:00

pages

227-36

issue

1

eissn

0014-4827

issn

1090-2422

pii

0014-4827(83)90123-4

journal_volume

143

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Caspase activity is not sufficient to execute cell death.

    abstract：:Molecular studies of the physiological cell death process have focused attention on the role of effector caspases as critical common elements of the lethal mechanism. Diverse death signals act afferently via distinct signaling pathways to activate these resident proenzyme molecules post-translationally. Whether this m...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(03)00289-1

    authors： Lukovic D,Komoriya A,Packard BZ,Ucker DS

    更新日期：2003-10-01 00:00:00

  • Establishment of a germ-line competent C57BL/6 embryonic stem cell line.

    abstract：:Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90430-3

    authors： Ledermann B,Bürki K

    更新日期：1991-12-01 00:00:00

  • Vectorial transcytosis of immunoglobulin G by human term trophoblast cells in culture.

    abstract：:Trophoblast cells were grown on filters that allow access to apical and basal surfaces of cells. Using this experimental system, IgG transport was shown to be specific and to occur primarily in the apical to basal direction. This transport was time- and temperature-dependent, with approximately 10% of added IgG appear...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90154-m

    authors： Sooranna SR,Contractor SF

    更新日期：1991-01-01 00:00:00

  • Analysis of mtDNA copy number and composition of single mitochondrial particles using flow cytometry and PCR.

    abstract：:Mitochondrial DNA (mtDNA) is a multicopy, maternally inherited, genome. Individuals frequently carry a mixture of genetically distinct mtDNA molecules whose proportions may vary between sexual generations or among tissues from the same individual. Analyses of the genetic composition of mitochondria have previously rel...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4949

    authors： Cavelier L,Johannisson A,Gyllensten U

    更新日期：2000-08-25 00:00:00

  • Characterization and response of newly developed high-grade glioma cultures to the tyrosine kinase inhibitors, erlotinib, gefitinib and imatinib.

    abstract：:High-grade gliomas (HGG), are the most common aggressive brain tumours in adults. Inhibitors targeting growth factor signalling pathways in glioma have shown a low clinical response rate. To accurately evaluate response to targeted therapies further in vitro studies are necessary. Growth factor pathway expression usin...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2012.01.014

    authors： Kinsella P,Howley R,Doolan P,Clarke C,Madden SF,Clynes M,Farrell M,Amberger-Murphy V

    更新日期：2012-03-10 00:00:00

  • Antiangiogenesis in cancer therapy--endostatin and its mechanisms of action.

    abstract：:The first angiogenesis inhibitors for cancer have now been approved by the F.D.A. in the U.S. and in 28 other countries, including China. The majority of these are monotherapies that block VEGF. However, mutant tumor cells may over time produce redundant angiogenic factors. Therefore, for long-term use in cancer, comb...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2005.11.015

    authors： Folkman J

    更新日期：2006-03-10 00:00:00

  • Growth factor requirements of normal and polyomavirus middle T gene transformed REF52 cells in serum-free medium: indications of a reduced vasopressin requirement and its relationship to the control of phosphatidylinositol metabolism.

    abstract：:The growth factor requirement of normal and polyomavirus middle T gene transformed REF52 cells was studied in serum-free medium in an attempt to elucidate the possible linkage between an altered growth factor requirement and one or more altered physiological properties of the transformed cells. For optimal growth, REF...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90432-1

    authors： Li L,Orlando J,Chen JK

    更新日期：1989-07-01 00:00:00

  • Insulin suppresses distal-less homeobox 5 expression through the up-regulation of microRNA-124 in 3T3-L1 cells.

    abstract：:Distal-less homeobox 5 (Dlx5) is a pro-osteogenic but anti-adipogenic transcription factor that regulates lineage commitment in mesenchymal stem cells. Although the expression of Dlx5 is known to be decreased by adipogenic stimuli, the mechanism of Dlx5 down-regulation has not yet been clarified. MicroRNAs (miRNAs) ar...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.04.020

    authors： Qadir AS,Woo KM,Ryoo HM,Baek JH

    更新日期：2013-08-15 00:00:00

  • Enhanced expression of heat shock proteins in gradually dying cells and their release from necrotically dead cells.

    abstract：:Heat shock proteins (HSPs) have molecular chaperone functions in protein biogenesis as well as cytoprotective functions against deleterious environmental stresses, and they work mainly inside of the cells. HSPs are usually induced in living cells that have been exposed to mild stresses or have recovered from severe st...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.07.014

    authors： Saito K,Dai Y,Ohtsuka K

    更新日期：2005-10-15 00:00:00

  • Molecular mechanisms of mechanotransduction in integrin-mediated cell-matrix adhesion.

    abstract：:Cell-matrix adhesion complexes are multi-protein structures linking the extracellular matrix (ECM) to the cytoskeleton. They are essential to both cell motility and function by bidirectionally sensing and transmitting mechanical and biochemical stimulations. Several types of cell-matrix adhesions have been identified ...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2016.10.001

    authors： Li Z,Lee H,Zhu C

    更新日期：2016-11-15 00:00:00

  • A tubulin identified by a monoclonal antibody is not present in mitotic spindles.

    abstract：:A monoclonal antibody, G8, which recognizes a form of tubulin (G8-tubulin) with a novel distribution in Rat-1 cells and Potorous tridactylis kidney (Ptk-2) cells was isolated. G8 labeled the interphase cytoskeleton of Rat-1 fibroblasts but not mitotic spindles or midbodies. G8 also stained a fiber network in some but ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90345-5

    authors： Gossels JM,Ingram VM

    更新日期：1989-10-01 00:00:00

  • The antioxidant, rather than prooxidant, activities of quercetin on normal cells: quercetin protects mouse thymocytes from glucose oxidase-mediated apoptosis.

    abstract：:The bioflavonoid quercetin is a dietary anticancer chemical that is capable of inducing apoptosis in tumor cells. Although the activity of quercetin is believed to be due to its antioxidative properties, it has recently been suggested that quercetin also has prooxidant activities, which might effect cytotoxicity direc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(03)00410-5

    authors： Lee JC,Kim J,Park JK,Chung GH,Jang YS

    更新日期：2003-12-10 00:00:00

  • Probing effects of pH change on dynamic response of Claudin-2 mediated adhesion using single molecule force spectroscopy.

    abstract：:Claudins belong to a large family of transmembrane proteins that localize at tight junctions (TJs) where they play a central role in regulating paracellular transport of solutes and nutrients across epithelial monolayers. Their ability to regulate the paracellular pathway is highly influenced by changes in extracellul...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.05.015

    authors： Lim TS,Vedula SR,Hui S,Kausalya PJ,Hunziker W,Lim CT

    更新日期：2008-08-15 00:00:00

  • SIRT6 inhibits TNF-α-induced inflammation of vascular adventitial fibroblasts through ROS and Akt signaling pathway.

    abstract：:SIRT6, with both deacetylase and ADP-ribosyltransferase activities, is predominantly expressed in the nucleus. It has been revealed that SIRT6 regulates various biological functions including metabolism, aging and stress resistance. This study aims to investigate the role of SIRT6 in vascular inflammation and it molec...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.05.001

    authors： He Y,Xiao Y,Yang X,Li Y,Wang B,Yao F,Shang C,Jin Z,Wang W,Lin R

    更新日期：2017-08-01 00:00:00

  • Recombinant human IgG antibodies recognizing distinct extracellular domains of EGF receptor exhibit different degrees of growth inhibitory effects on human A431 cancer cells.

    abstract：:Recently, we isolated 4 distinct kinds of single chain antibody against human EGF receptor (EGFR) after screening the Keio phage display scFv library by using two methods of target-guided proximity labeling. In the current study, these monovalent scFv antibodies were converted to bivalent IgGs of humanized forms (hIgG...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.03.002

    authors： Chang C,Takayanagi A,Yoshida T,Shimizu N

    更新日期：2013-05-01 00:00:00

  • CXCR4 and CXCL12 are inversely expressed in colorectal cancer cells and modulate cancer cell migration, invasion and MMP-9 activation.

    abstract：:Colorectal cancer (CRC) is characterized by a distinct metastatic pattern resembling chemokine-induced leukocyte trafficking. This prompted us to investigate expression, signal transduction and specific functions of the chemokine receptor CXCR4 in CRC cells and metastases. Using RT-PCR analysis and Western blotting, w...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.07.006

    authors： Brand S,Dambacher J,Beigel F,Olszak T,Diebold J,Otte JM,Göke B,Eichhorst ST

    更新日期：2005-10-15 00:00:00

  • Intracellular and extracellular cathepsin B facilitate invasion of MCF-10A neoT cells through reconstituted extracellular matrix in vitro.

    abstract：:Lysosomal cysteine proteinase cathepsin B is implicated in remodeling the extracellular matrix, a crucial step in the process of tumor cell invasion. In this study the contributions of intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT,...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(02)00055-1

    authors： Premzl A,Zavasnik-Bergant V,Turk V,Kos J

    更新日期：2003-02-15 00:00:00

  • Adhesion of human amnion epithelial cells to extracellular matrix. Evidence for multiple mechanisms.

    abstract：:Human amnion epithelial cells attach and flatten slowly (approximately 65 min) onto plastic in the presence of serum but much more rapidly (20-30 min) onto subcellular matrix (SCM) deposited by the same cells. This matrix contains both fibronectin and laminin, but neither molecule on its own can reproduce its adhesive...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90611-6

    authors： Aplin JD,Campbell S,Foden LJ

    更新日期：1984-08-01 00:00:00

  • Oxysterol-binding protein-related protein (ORP) 6 localizes to the ER and ER-plasma membrane contact sites and is involved in the turnover of PI4P in cerebellar granule neurons.

    abstract：:Oxysterol-binding protein (OSBP)-related proteins (ORPs) are conserved lipid binding proteins found in organisms ranging from yeast to mammals. Recent findings have indicated that these proteins mainly localize to contact sites of 2 different membranous organelles. ORP6, a member of the ORP subfamily III, is one of th...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.07.025

    authors： Mochizuki S,Miki H,Zhou R,Kido Y,Nishimura W,Kikuchi M,Noda Y

    更新日期：2018-09-15 00:00:00

  • Immortalisation of human ovarian surface epithelium with telomerase and temperature-sensitive SV40 large T antigen.

    abstract：:Epithelial ovarian cancer is the most common form of gynaecological malignancy. This lethal disease is thought to arise in ovarian surface epithelial (OSE) cells. The biology of these cells is not well understood, due to the limited amount of tissue that can be obtained from a single biopsy and their limited life span...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(03)00218-0

    authors： Davies BR,Steele IA,Edmondson RJ,Zwolinski SA,Saretzki G,von Zglinicki T,O'Hare MJ

    更新日期：2003-08-15 00:00:00

  • The long non-coding RNA ILF3-AS1 increases the proliferation and invasion of retinoblastoma through the miR-132-3p/SMAD2 axis.

    abstract：:A great deal of evidence suggests that long non-coding RNAs (lncRNAs) function in the tumorigenesis of retinoblastoma (RB). However, the roles of lncRNA ILF3-AS1 in RB are still unclear. In the present study, our work revealed that the lncRNA ILF3-AS1 was increased in both RB tissues and cell lines. Repression of ILF3...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.112087

    authors： Han S,Song L,Chen Y,Hou M,Wei X,Fan D

    更新日期：2020-08-15 00:00:00

  • Do IL-3/GM-CSF effect on the myofibroblastic differentiation of human adipose derived stromal cells?

    abstract：BACKGROUND:Capsular contracture is an incurable complication after silicone-based implant surgery. Myofibroblast is the predominant cell in the contracted capsule. We hypothesized that human adipose derive stromal cells (hASCs) together with fibroblast may show a similar phenotypic characteristics of myofibroblast afte...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.03.056

    authors： Lee JS,Tae SS,Kim DY,Han SK,Kim WK,Dhong ES

    更新日期：2017-06-15 00:00:00

  • Eps8 is recruited to lysosomes and subjected to chaperone-mediated autophagy in cancer cells.

    abstract：:Eps8 controls actin dynamics directly through its barbed end capping and actin-bundling activity, and indirectly by regulating Rac-activation when engaged into a trimeric complex with Eps8-Abi1-Sos1. Recently, Eps8 has been associated with promotion of various solid malignancies, but neither its mechanisms of action n...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.02.020

    authors： Welsch T,Younsi A,Disanza A,Rodriguez JA,Cuervo AM,Scita G,Schmidt J

    更新日期：2010-07-15 00:00:00

  • Effect of transferrin on alkaline phosphatase activity and collagen synthesis in osteoblastic cells derived from newborn mouse calvaria.

    abstract：:The effect of transferrin was tested on osteoblastic cells (clone MC3T3-E1) cultured in serum-free medium containing 1% bovine serum albumin (BSA). Transferrin (Tf) stimulated increases of protein content and protein synthesis, but not of DNA content and cell number, in the cells. This protein also increased alkaline ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90465-8

    authors： Tsunoi M,Hakeda Y,Kurihara N,Maeda N,Utsumi N,Kumegawa M

    更新日期：1984-07-01 00:00:00

  • Centrosomal and cytoplasmic Cdc2/cyclin B1 activation precedes nuclear mitotic events.

    abstract：:The activation of cdc2/cyclin B is the trigger for entry into mitosis. The mechanism of cdc2/cyclin B activation is complex, but the final step is the dephosphorylation of the Thr14 and Tyr15 residues on the cdc2 subunit, catalyzed by a member of the Cdc25 family of phosphatases. Cdc2/cyclin B1 accumulates at the cent...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4872

    authors： De Souza CP,Ellem KA,Gabrielli BG

    更新日期：2000-05-25 00:00:00

  • Immunodetection of poly(A) binding protein II in the cell nucleus.

    abstract：:During the polyadenylation of pre-mRNA in vitro, poly(A) binding protein II (PAB II) binds to the growing poly(A) tail, stimulating its extension. The subcellular localization of PAB II was investigated with an antibody affinity-purified from rabbit serum raised against the purified protein. Immunofluorescence microsc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1235

    authors： Krause S,Fakan S,Weis K,Wahle E

    更新日期：1994-09-01 00:00:00

  • Cell-substrate interaction. A method for evaluating the possible correlation between metastatic phenotype and cell surface energy.

    abstract：:The interfacial energy of the non-attached to substrate cell surface was analysed in tumor cell variants of the K-1735 melanoma and UV-2237 fibrosarcoma series, which exhibit distinct metastatic phenotypes. The highly metastatic cell variants exhibited a two-fold increase in the ability to form rapid cell substrate in...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90625-6

    authors： Reich S,Rosin H,Levy M,Karkash R,Raz A

    更新日期：1984-08-01 00:00:00

  • PLCgamma participates in insulin stimulation of glucose uptake through activation of PKCzeta in brown adipocytes.

    abstract：:Based on recent studies showing that PLCgamma associates to insulin receptor, we investigated its role in insulin stimulation of glucose transport in brown adipocytes. Insulin stimulation induced rapid PLCgamma association to phosphorylated insulin receptor, and activation of PLCgamma, as assessed by the mobilization ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5570

    authors： Lorenzo M,Teruel T,Hernandez R,Kayali AG,Webster NJ

    更新日期：2002-08-15 00:00:00

  • Signaling and subcellular localization of the TNF receptor Edar.

    abstract：:Tabby and downless mutant mice have identical phenotypes characterized by deficient development of several ectodermally derived organs such as teeth, hair, and sweat glands. Edar, encoded by the mouse downless gene and defective in human dominant and recessive forms of autosomal hypohidrotic ectodermal dysplasia (EDA)...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5331

    authors： Koppinen P,Pispa J,Laurikkala J,Thesleff I,Mikkola ML

    更新日期：2001-10-01 00:00:00

  • Transforming growth factor-beta 1 has both promoting and inhibiting effects on induction of DNA synthesis in human fibroblasts.

    abstract：:Growth-arrested human normal fibroblasts, TIG-1, initiated DNA synthesis following addition of epidermal growth factor (EGF). Transforming growth factor-beta 1 (TGF-beta 1) by itself had no effect on induction of DNA synthesis. When EGF and TGF-beta 1 were added simultaneously to growth-arrested TIG-1 cells, induction...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1014

    authors： Fukami J,Tsuji K,Ueno A,Ide T

    更新日期：1995-01-01 00:00:00