Abstract:
:Human amnion epithelial cells attach and flatten slowly (approximately 65 min) onto plastic in the presence of serum but much more rapidly (20-30 min) onto subcellular matrix (SCM) deposited by the same cells. This matrix contains both fibronectin and laminin, but neither molecule on its own can reproduce its adhesive properties. Cells attach on surfaces containing fibronectin and laminin and extend filopodial and lamellipodial areas of cytoplasm without extensive flattening in the perinuclear region. Matrix deposited onto plastic by amnion epithelial cells has trypsin-sensitive and trypsin-resistant, papain-sensitive adhesion-promoting components. Cell spreading triggered by the latter but not the former can be inhibited by pretreating the adhering cells with heparin. Other GAGs are without effect. The results are discussed in terms of multiple interactions between epithelial cells and basal laminae.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Aplin JD,Campbell S,Foden LJdoi
10.1016/0014-4827(84)90611-6subject
Has Abstractpub_date
1984-08-01 00:00:00pages
425-38issue
2eissn
0014-4827issn
1090-2422journal_volume
153pub_type
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