Abstract:
:The involvement of p53 as a determinant of chemosensitivity or radiosensitivity is not well understood and is complicated by numerous contradictory reports. Here we have addressed this issue using a series of isogenic clones derived from two neuroblastoma cell lines that express wild-type p53 genes, Nub7 and IMR32. Two different mutant p53 transgenes were used in an attempt to disrupt p53 function in the clones. Our findings indicate that the cellular response is dependent on the genotoxic agent used as well as on the specific p53 transgene used. Cellular radiosensitivity showed no association with apoptosis or with the ability of the cells to arrest in G1 after irradiation. An association was observed, however, between gamma-radiation sensitivity and DNA double-strand break rejoining activity.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Gangopadhyay S,Jalali F,Reda D,Peacock J,Bristow RG,Benchimol Sdoi
10.1006/excr.2002.5493keywords:
subject
Has Abstractpub_date
2002-04-15 00:00:00pages
122-31issue
1eissn
0014-4827issn
1090-2422pii
S0014482702954935journal_volume
275pub_type
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