The intracellular domain of Jagged-1 interacts with Notch1 intracellular domain and promotes its degradation through Fbw7 E3 ligase.


:Notch signaling involves the proteolytic cleavage of the transmembrane Notch receptor after binding to its transmembrane ligands. Jagged-1 also undergoes proteolytic cleavage by gamma-secretase and releases an intracellular fragment. In this study, we have demonstrated that the Jagged-1 intracellular domain (JICD) inhibits Notch1 signaling via a reduction in the protein stability of the Notch1 intracellular domain (Notch1-IC). The formation of the Notch1-IC-RBP-Jk-Mastermind complex is prevented in the presence of JICD, via a physical interaction. Furthermore, JICD accelerates the protein degradation of Notch1-IC via Fbw7-dependent proteasomal pathway. These results indicate that JICD functions as a negative regulator in Notch1 signaling via the promotion of Notch1-IC degradation.


Exp Cell Res


Kim MY,Jung J,Mo JS,Ann EJ,Ahn JS,Yoon JH,Park HS




Has Abstract


2011-10-15 00:00:00














  • The β-catenin signaling pathway induces aggressive potential in breast cancer by up-regulating the chemokine CCL5.

    abstract::β-Catenin signaling plays a pivotal role in the genesis of a variety of malignant tumors, but its role in breast cancer has not been fully elucidated. Here, we examined whether deregulation of β-catenin signaling is related to the aggressive characteristics of certain types of breast cancers. Analysis of cytokine leve...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yasuhara R,Irié T,Suzuki K,Sawada T,Miwa N,Sasaki A,Tsunoda Y,Nakamura S,Mishima K

    更新日期:2015-10-15 00:00:00

  • The glomerular basement membrane.

    abstract::The kidney's glomerular filtration barrier consists of two cells-podocytes and endothelial cells-and the glomerular basement membrane (GBM), a specialized extracellular matrix that lies between them. Like all basement membranes, the GBM consists mainly of laminin, type IV collagen, nidogen, and heparan sulfate proteog...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Miner JH

    更新日期:2012-05-15 00:00:00

  • Serum and serotonin induce retraction of calf aortic smooth muscle (CASM) cells in vitro: inhibition by ketanserin, a 5-HT2 receptor antagonist.

    abstract::Calf aortic smooth muscle (CASM) cells cultured in vitro at high cell density (4 x 10(4) cells/cm2) on bacteriological petri dishes in the presence of serum pile up in clusters and create open spaces in the monolayer. This phenomenon is clearly visible 6 days after plating and is markedly enhanced by the addition of f...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: De Wever B,Rooman RP,De Brabander M

    更新日期:1990-01-01 00:00:00

  • Involvement of JNK signaling in IL4-induced M2 macrophage polarization.

    abstract::It has been generally accepted that alternatively activated macrophages (M2), which can be induced by type 2 cytokines such as IL-4, is responsible for tissue repair. However, the function of JNK in IL-4-induced M2 macrophage polarization remains unclear. Here, we demonstrated that M0 macrophages can be polarized into...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hao J,Hu Y,Li Y,Zhou Q,Lv X

    更新日期:2017-08-15 00:00:00

  • TRIP6 accelerates the proliferation and invasion of cervical cancer by upregulating oncogenic YAP signaling.

    abstract::Accumulating evidence has suggested that thyroid hormone receptor interacting protein 6 (TRIP6) is a novel tumor-related regulator that is aberrantly expressed in multiple tumors and contributes to tumor progression and metastasis. Yet, little is known about the role of TRIP6 in cervical cancer. In the current study, ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yang F,Li L,Zhang J,Zhang J,Yang L

    更新日期:2020-11-01 00:00:00

  • Telomere length in fibroblasts and blood cells from healthy centenarians.

    abstract::Several lines of evidence indicate that telomere shortening during in vitro aging of human somatic cells plays a causal role in cellular senescence. A critical telomere length seems to be associated with the replicative block characterizing senescent cells. In this paper we analyzed the mean length of the terminal res...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mondello C,Petropoulou C,Monti D,Gonos ES,Franceschi C,Nuzzo F

    更新日期:1999-04-10 00:00:00

  • 24R,25-dihydroxyvitamin D3 increases cyclic GMP contents, leading to an enhancement of osteocalcin synthesis by 1,25-dihydroxyvitamin D3 in cultured human osteoblastic cells.

    abstract::The effect of the physiological vitamin D metabolite 24R, 25-dihydroxyvitamin D3 [24R,25(OH)2D3] on human osteoblastic cells was assessed. Physiological concentrations (10(-9)-10(-8) M) of 24R, 25(OH)2D3 significantly increased the cyclic guanosine 5'-monophosphate (cGMP) content in the human osteoblastic cells by app...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yamamoto T,Ozono K,Shima M,Yamaoka K,Okada S

    更新日期:1998-10-10 00:00:00

  • Permeable FGF-1 nuclear localization signal peptide stimulates DNA synthesis in various cell types but is cell-density sensitive and unable to support cell proliferation.

    abstract::An earlier report indicated that a 26-amino-acid peptide (SA), comprised of the nuclear localization signal (NLS) of fibroblast growth factor-1 (FGF-1) and a membrane-permeable peptide, was able to stimulate DNA synthesis after it was taken up by NIH3T3 fibroblasts. Here, we report that SA, but not a mutant with the N...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Komi A,Suzuki M,Imamura T

    更新日期:1998-09-15 00:00:00

  • Down-regulation of RBP-J mediated by microRNA-133a suppresses dendritic cells and functions as a potential tumor suppressor in osteosarcoma.

    abstract:BACKGROUND AND OBJECTIVE:In recent years, immunotherapy for the treatment of tumors have been established. Dendritic cells (DCs) are extremely efficient and professional antigen presenting cells (APCs), which are an important target for immune therapeutic interventions in cancer. In present study, we investigated wheth...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gao X,Han D,Fan W

    更新日期:2016-12-10 00:00:00

  • Immunopurification of a sarcomeric junctional protein complex containing GAPDH.

    abstract::We have isolated a monoclonal antibody, P4B2, which localizes to multiple anchorage junctions, namely, a subset of focal adhesions, the Z-disk of muscle, and neuromuscular junctions. Immunopurification of the antigen to this antibody from chicken brain tissue yielded a complex of three prominent proteins with mobiliti...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: McDonald KA,Muschler J,Horwitz AF

    更新日期:1998-09-15 00:00:00

  • Thrombin peptide, TP508, induces differential gene expression in fibroblasts through a nonproteolytic activation pathway.

    abstract::Prior studies have shown that synthetic peptides representing the domain of thrombin responsible for high-affinity binding to fibroblasts stimulate chemotactic and cell proliferative signals through a nonproteolytic mechanism. One of these peptides, TP508, has recently been shown to be chemotactic for neutrophils, to ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sower LE,Payne DA,Meyers R,Carney DH

    更新日期:1999-03-15 00:00:00

  • The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis.

    abstract::Hyperglycemia and hyperlipidemia (glycolipotoxicity)-triggered islet β-cell dysfunction is known to drive the progression of obesity-related type 2 diabetes, however the underlying mechanisms have not been clearly elucidated. The current study aimed to investigate the role of mitogen-activated protein kinase phosphata...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Song Z,Ma J,Lu Y,Zhou C,Zhao T,Ai X,Wei X,Lin J,Wang W,Yan W,Jiao P

    更新日期:2019-09-01 00:00:00

  • Colony-stimulating factor 1 (CSF-1) is involved in an autocrine growth control of rat myogenic cells.

    abstract::Colony stimulating factor-1 (CSF-1) and the CSF-1 receptor (the c-fms proto-oncogene product) are expressed during the proliferation of the L6 alpha 1 rat myogenic cell line and both are down-regulated during the differentiation to myotubes. Biologically active CSF-1 was shown to be secreted into the culture medium by...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Borycki AG,Smadja F,Stanley R,Leibovitch SA

    更新日期:1995-05-01 00:00:00

  • Developmental regulation of elongation factor-1 delta in sea urchin suggests appearance of a mechanism for alternative poly(A) site selection in gastrulae.

    abstract::Elongation factor-1 delta gene expression was analyzed during sea urchin development. EF-1 delta mRNA is present as a single 2.7-kb transcript in unfertilized eggs and in rapidly dividing cleavage stage embryos. It decreases rapidly 6 h after fertilization and then reappears at the gastrula stage as two transcripts of...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Delalande C,Monnier A,Minella O,Genevière AM,Mulner-Lorillon O,Bellé R,Cormier P

    更新日期:1998-07-10 00:00:00

  • A RanBP1 mutation which does not visibly affect nuclear import may reveal additional functions of the ran GTPase system.

    abstract::Ran, a nuclear GTPase, and a number of interacting proteins, including regulators RanGEF1 and RanGAP1, are involved in nucleocytoplasmic transport. We have identified a new temperature-sensitive mutation in budding yeast YRB1 gene, which encodes Ran-binding protein-1 (RanBP1). In contrast to other yrb1 alleles, the ne...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ouspenski II

    更新日期:1998-10-10 00:00:00

  • The role of long non-coding RNA H19 in musculoskeletal system: A new player in an old game.

    abstract::The long non-coding RNAs (lncRNAs) have gained much attention due to its essential roles in molecular regulation. As one of the classic lncRNAs, H19 is strongly expressed during embryogenesis and plays a crucial biological function during development. Mesenchymal stem cells (MSCs) are an ideal cell source for tissue e...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Liu Y,Li G,Zhang JF

    更新日期:2017-11-15 00:00:00

  • Activation of a cAMP pathway and induction of melanogenesis correlate with association of p16(INK4) and p27(KIP1) to CDKs, loss of E2F-binding activity, and premature senescence of human melanocytes.

    abstract::There is strong evidence that the senescent phenotype, whether induced by telomere shortening, oxidative damage, or oncogenic stimuli, is an important tumor suppressive mechanism. The melanocyte is a cell of neural crest origin that produces the pigment melanin and can develop into malignant melanomas. To understand h...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Haddad MM,Xu W,Schwahn DJ,Liao F,Medrano EE

    更新日期:1999-12-15 00:00:00

  • Effects of polyamines on the first division cycle of Xenopus laevis eggs.

    abstract::The involvement of polyamines in cytokinesis has been examined in eggs of the amphibian X. laevis. Microinjection of spermine or spermidine into unfertilized eggs induced a shortening of the first division cycle and early formation of cleavage-like constrictions. Eggs were activated by injection and developed furrows ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Grant NJ,Aimar C,Oriol-Audit C

    更新日期:1984-02-01 00:00:00

  • The blocked pinocytic activity of mitotic cells is restored in mitotic-interphase hybrids.

    abstract::During mitosis there is an abrupt inhibition of a wide range of membrane functions, including fluid-phase and adsorptive pinocytosis. We have used cell hybrids formed between mitotic and interphase cells to approach the mechanism of this inhibition. We report that fluid pinocytosis is reactivated in the mitotic partne...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Oliver JM,Seagrave JC

    更新日期:1987-08-01 00:00:00

  • A C-terminal PDZ binding domain modulates the function and localization of Kv1.3 channels.

    abstract::The voltage-gated potassium channel, Kv1.3, plays an important role in regulating membrane excitability in diverse cell types ranging from T-lymphocytes to neurons. In the present study, we test the hypothesis that the C-terminal PDZ binding domain modulates the function and localization of Kv1.3. We created a mutant ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Doczi MA,Damon DH,Morielli AD

    更新日期:2011-10-01 00:00:00

  • Detection in BHK cells of a precursor form for lamin A.

    abstract::Lamins are structural proteins found in the fibrous lamina underlining the nuclear envelope. In vitro translation of polyadenylated RNA or polysomes followed by immunoprecipitation with a serum raised against BHK nuclear matrix proteins showed that lamin A (72 kD) is synthesized as a high molecular weight precursor (7...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Dagenais A,Bibor-Hardy V,Laliberté JF,Royal A,Simard R

    更新日期:1985-12-01 00:00:00

  • The tumor suppressor protein p16INK4a.

    abstract::The tumor suppressor protein p16INK4a (inhibitor of CDK4) is one of the most direct links between cell-cycle control and cancer. The p16INK4a gene is frequently inactivated in human tumors, and inheritance of mutant alleles results in susceptibility to several types of cancer. p16INK4a is part of a cell-cycle regulato...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Serrano M

    更新日期:1997-11-25 00:00:00

  • Annexin VI is a mannose-6-phosphate-independent endocytic receptor for bovine β-glucuronidase.

    abstract::Endocytosis and transport of bovine liver β-glucuronidase to lysosomes in human fibroblasts are mediated by two receptors: the well-characterized cation-independent mannose 6-phosphate receptor (IGF-II/Man6PR) and an IGF-II/Man6PR-independent receptor, which recognizes a Ser-Trp*-Ser sequence present on the ligand. Th...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ramírez-Mata A,Michalak C,Mendoza-Hernández G,León-Del-Río A,González-Noriega A

    更新日期:2011-10-01 00:00:00

  • Residues in the alternative reading frame tumor suppressor that influence its stability and p53-independent activities.

    abstract::The Alternative Reading Frame (ARF) protein suppresses tumorigenesis through p53-dependent and p53-independent pathways. Most of ARF's anti-proliferative activity is conferred by sequences in its first exon. Previous work showed specific amino acid changes occurred in that region during primate evolution, so we progra...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: di Tommaso A,Hagen J,Tompkins V,Muniz V,Dudakovic A,Kitzis A,Ladeveze V,Quelle DE

    更新日期:2009-04-15 00:00:00

  • The role of autophagy in THP-1 macrophages resistance to HIV- vpr-induced apoptosis.

    abstract::Macrophages are resistant to cell death and are one of HIV reservoirs. HIV viral protein Vpr has the potential to promote infection of and survival of macrophages, which could be a highly significant factor in the development and/or maintenance of macrophage viral reservoirs. However, the impact of vpr on macrophages ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhou HY,Zheng YH,He Y,Chen Z,He B

    更新日期:2017-02-01 00:00:00

  • Nuclear actin filaments and their topological changes in frog oocytes.

    abstract::Previous morphological and biochemical studies have suggested that actin and actin-containing filaments (microfilaments) exist in the eukaryotic nucleus and that they perform important nuclear functions. However, the concept is not widely accepted. In this study, we demonstrate actin and bundles of actin in the nuclei...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Parfenov VN,Davis DS,Pochukalina GN,Sample CE,Bugaeva EA,Murti KG

    更新日期:1995-04-01 00:00:00

  • Toxic peptides in Frazer's fraction interact with the actin cytoskeleton and affect the targeting and function of intestinal proteins.

    abstract::Celiac disease (CD) is a multisystemic autoimmune inflammation of the intestinal tract induced by wheat gluten and related cereals in HLA-DQ2/8 positive individuals. An essential role in the pathogenesis of CD is played by a fraction of the peptic-tryptic digest of gluten, Frazer's Fraction (FF). Here, we investigate ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Reinke Y,Zimmer KP,Naim HY

    更新日期:2009-11-15 00:00:00

  • Assembly of the nucleolar precursor bodies in human male pronuclei is correlated with an early RNA synthetic activity.

    abstract::The nucleolar precursor bodies (NPBs) are electrondense, homogeneous, and finely filamentous intranuclear structures occurring in mammalian zygotes during the early postfertilization development and subsequently transforming into nucleoli. In this study we addressed the question if the assembly of the NPBs is correlat...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tesarik J,Kopecny V

    更新日期:1990-11-01 00:00:00

  • Gap junctional communication of primary human keratinocytes: characterization by dual voltage clamp and dye transfer.

    abstract::We have compared dye coupling in pairs of small (less than 10 microns in diameter) and large (greater than 20 microns in diameter) keratinocytes isolated from normal human epidermis, using Lucifer yellow microinjection. Under control conditions, dye coupling was found in only 1 out of the 25 small pairs tested, wherea...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Salomon D,Chanson M,Vischer S,Masgrau E,Vozzi C,Saurat JH,Spray DC,Meda P

    更新日期:1992-08-01 00:00:00

  • Towards a further understanding of the growth-inhibiting action of oxygen deficiency. Evaluation of the effect of antimycin on proliferating Ehrlich ascites tumour cells.

    abstract::The effect of 1 microM antimycin on the proliferative properties, metabolism and basic cell composition of Ehrlich ascites tumour cells cultured in the second in vitro passage was studied. Continuous drug exposure of asynchronous cells caused rapid cessation of cell growth, characterized by the cell number and DNA, RN...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Löffler M

    更新日期:1985-03-01 00:00:00