Altered structure of ribosomal RNA transcription units in hamster cells after DRB treatment.

Abstract:

:The adenosine analog 5,6 dichloro-1-beta-ribofuranosyl benzimidazole (DRB) inhibits specifically mRNA production in eukaryotic cells, with concomitant alterations in pre-rRNA processing and nucleolar ultrastructure. We have focused our analysis on the effects of drug treatment on rRNA transcription through observations of spread ribosomal RNA (rRNA) transcription units from hamster cells. A significant increase in the size of ribosomal matrix units is paralleled by the appearance of abnormally large RNP structures containing elongating pre-rRNA. These structures correspond to nucleolar perichromatin granules which are known to appear in various conditions of cell growth when rRNA processing is altered. It is proposed that DRB-induced impairments in rRNA maturation are largely determined by alterations in the rRNA transcription process.

journal_name

Exp Cell Res

authors

Puvion-Dutilleul F,Nicoloso M,Bachellerie JP

doi

10.1016/0014-4827(83)90322-1

subject

Has Abstract

pub_date

1983-06-01 00:00:00

pages

43-52

issue

1

eissn

0014-4827

issn

1090-2422

pii

0014-4827(83)90322-1

journal_volume

146

pub_type

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