Abstract：
:Autophagy is a major catabolic process allowing the renewal of intracellular organelles by which cells maintain their homeostasis. We have previously shown that autophagy is controlled by two transduction pathways mediated by a heterotrimeric Gi3 protein and phosphatidylinositol 3-kinase activities in the human colon cancer cell line HT-29. Here, we show that 3-methyladenine, an inhibitor of autophagy, increases the sensitivity of HT-29 cells to apoptosis induced by sulindac sulfide, a nonsteroidal anti-inflammatory drug which inhibits the cyclooxygenases. Similarly, HT-29 cells overexpressing a GTPase-deficient mutant of the G(alpha i3) protein (Q204L), which have a low rate of autophagy, were more sensitive to sulindac sulfide-induced apoptosis than parental HT-29 cells. In both cell populations we did not observe differences in the expression patterns of COX-2, Bcl-2, Bcl(XL), Bax, and Akt/PKB activity. However, the rate of cytochrome c release was higher in Q204L-overexpressing cells than in HT-29 cells. These results suggest that autophagy could retard apoptosis in colon cancer cells by sequestering mitochondrial death-promoting factors such as cytochrome c.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Bauvy C,Gane P,Arico S,Codogno P,Ogier-Denis Edoi
10.1006/excr.2001.5285keywords:
subject
Has Abstractpub_date
2001-08-15 00:00:00pages
139-49issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(01)95285-1journal_volume
268pub_type
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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更新日期：2006-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1998-11-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1006/excr.1994.1051
更新日期：1994-03-01 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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