Abstract:
:Despite an increasing interest in the role of the p53 tumour suppressor protein in embryonic stem cells, not much is known about its regulation in this cell type. We show that the relatively high amount of p53 protein correlates with a higher amount of p53 RNA in ES cells compared to differentiated cells. Moreover, p53 RNA is more stable in embryonic stem cells and the p53 protein is more often transcribed. This is at least partly due to decreased expression of miRNA-125a and 125b in embryonic stem cells. Despite its cytoplasmic localisation, p53 is degraded in 26S proteasomes in embryonic stem cells. This process is controlled by Mdm2, the deubiquitinating enzyme Hausp and Ubc13. In contrast, the E3 ligase PirH2 appears to be less important for the control of p53 in embryonic stem cells. During differentiation, p53 protein and RNA levels are decreased which corresponds to increased expression of miRNA-125a and miRNA-125b.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Solozobova V,Blattner Cdoi
10.1016/j.yexcr.2010.06.006subject
Has Abstractpub_date
2010-09-10 00:00:00pages
2434-46issue
15eissn
0014-4827issn
1090-2422pii
S0014-4827(10)00315-0journal_volume
316pub_type
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