当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Development of a chemically defined serum-free medium for differentiation of rat adipose precursor cells.

Development of a chemically defined serum-free medium for differentiation of rat adipose precursor cells.

Abstract：

:Stromal-vascular cells from the epididymal fat pad of 4-week-old rats, when cultured in a medium containing insulin or insulin-like growth factor, IGF-I, triiodothyronine and transferrin, were able to undergo adipose conversion. Over ninety percent of the cells accumulated lipid droplets and this proportion was reduced in serum-supplemented medium. The adipose conversion was assessed by the development of lipoprotein lipase (LPL) and glycerol-3-phosphate dehydrogenase (GPDH) activities, [14C]glucose incorporation into polar and neutral lipids, triacylglycerol accumulation and lipolysis in response to isoproterenol. Similar results were obtained with stromal-vascular cells from rat subcutaneous and retroperitoneal adipose tissues. Stromal-vascular cells required no adipogenic factors in addition to the components of the serum-free medium. Insulin was required within a physiological range of concentrations for the emergence of LPL and at higher concentrations for that of GPDH. When present at concentrations ranging from 2 to 50 nM, IGF-I was able to replace insulin for the expression of both LPL and GPDH. The development of a serum-free, chemically defined medium for the differentiation of diploid adipose precursor cells opens up the possibility of characterizing inhibitors or activators of the adipose conversion process.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Deslex S,Negrel R,Ailhaud G

doi

10.1016/0014-4827(87)90412-5

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

15-30

issue

1

eissn

0014-4827

issn

1090-2422

pii

0014-4827(87)90412-5

journal_volume

168

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Occurrence of proteinaceous 10-nm filaments throughout the cytoplasm of algae of the order Dasycladales.

    abstract：:Previously, whole-mount electron microscopy of nuclei extruded together with residual cytoplasm from the rhizoids of several algal species of the order Dasycladales has revealed the occurrence of an intra- and perinuclear network of 10-nm filaments morphologically indistinguishable from that of mammalian vimentin inte...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3924

    authors： Berger S,Wittke W,Traub P

    更新日期：1998-05-01 00:00:00

  • Identification of the type-B receptor for platelet-derived growth factor in human embryonal carcinoma cells.

    abstract：:The human embryonal carcinoma (EC) cell line Tera 2 clone 13 (T2/13) can be induced to differentiate in vitro into neuroectodermal cell types with retinoic acid. Undifferentiated cells are characterized by rapid proliferation, whereas differentiated cells show a prolonged generation time, have a limited life span, and...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90312-x

    authors： Weima SM,van Rooijen MA,Mummery CL,Feyen A,de Laat SW,van Zoelen EJ

    更新日期：1990-02-01 00:00:00

  • Establishment and characterization of a porcine B cell lymphoma cell line.

    abstract：:The lack of available, well characterized, established, domestic porcine cell lines hinders the advancement of porcine cellular immunology. A case of multicentric lymphoma was diagnosed in a market weight pig at the time of slaughter. Affected lymph nodes and spleen were collected and used for single cell isolation an...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.111986

    authors： Rahe MC,Dvorak CMT,Wiseman B,Martin D,Murtaugh MP

    更新日期：2020-05-15 00:00:00

  • Cellular responses to disruption of the permeability barrier in a three-dimensional organotypic epidermal model.

    abstract：:Repeated injury to the stratum corneum of mammalian skin (caused by friction, soaps, or organic solvents) elicits hyperkeratosis and epidermal thickening. Functionally, these changes serve to restore the cutaneous barrier and protect the organism. To better understand the molecular and cellular basis of this response,...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.04.021

    authors： Ajani G,Sato N,Mack JA,Maytin EV

    更新日期：2007-08-15 00:00:00

  • Hedgehog signaling contributes to basic fibroblast growth factor-regulated fibroblast migration.

    abstract：:Fibroblast migration is a central process in skin wound healing, which requires the coordination of several types of growth factors. bFGF, a well-known fibroblast growth factor (FGF), is able to accelerate fibroblast migration; however, the underlying mechanism of bFGF regulation fibroblast migration remains unclear. ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.03.054

    authors： Zhu ZX,Sun CC,Ting Zhu Y,Wang Y,Wang T,Chi LS,Cai WH,Zheng JY,Zhou X,Cong WT,Li XK,Jin LT

    更新日期：2017-06-15 00:00:00

  • Characterization of peroxisomes in Chinese hamster ovary cells in culture.

    abstract：:In order to explore the potential value of Chinese hamster ovary (CHO) cells for the isolation of peroxisomal mutants defective in the peroxisomal fatty acid oxidation system, some characteristics of their peroxisomes were studied. Catalase was detected biochemically and histochemically in peroxisome-like particles in...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90503-8

    authors： Santos MJ,Garrido J,Oliver C,Robbins AR,Leighton F

    更新日期：1985-11-01 00:00:00

  • The level of nerve growth factor (NGF) as a function of innervation. A correlation radio-immunoassay and bioassay study of the rat iris.

    abstract：:A two-site radio-immunoassay for beta NGF demonstrated 5-10 pg of NGF in the normal, adult rat iris. Ciliarectomy or sympathectomy did not significantly alter the amount of NGF after 10 days. However, denervation including all sensory axons (stereotactic lesion distal to the trigeminal ganglion) increased the level to...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90155-6

    authors： Ebendal T,Olson L,Seiger A

    更新日期：1983-10-15 00:00:00

  • A keratin of fetal skin is reexpressed in human keratinocytes transformed by SV40 virus or treated with the tumor promoter TPA.

    abstract：:SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90692-x

    authors： Darmon M,Delescluse C,Semat A,Bernard B,Bailly J,Prunieras M

    更新日期：1984-09-01 00:00:00

  • A novel bioactive membrane by cell electrospinning.

    abstract：:Electrospinning permits fabrication of biodegradable matrices that can resemble the both scale and mechanical behavior of the native extracellular matrix. However, achieving high-cellular density and infiltration of cells within matrices with traditional technique remain challenging and time consuming. The cell electr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.08.007

    authors： Chen H,Liu Y,Hu Q

    更新日期：2015-11-01 00:00:00

  • S632A3, a new glutarimide antibiotic, suppresses lipopolysaccharide-induced pro-inflammatory responses via inhibiting the activation of glycogen synthase kinase 3β.

    abstract：:Inflammatory mediators including inducible nitric oxide (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and Interleukin-6 (IL-6) contribute to the course of a variety of inflammatory diseases. S632A3 is a new member of the glutarimide antibiotics isolated from a cultured broth of Streptomyces hygrosc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2012.08.008

    authors： Deng H,Zhang N,Wang Y,Chen J,Shen J,Wang Z,Xu R,Zhang J,Song D,Li D

    更新日期：2012-12-10 00:00:00

  • In situ hybridization in living cells: detection of RNA molecules.

    abstract：:Fluorescence hybridization is a widely used technique in cell biology and pathology for detecting specific nucleic acid (DNA and RNA) sequences in fixed cells. This technique does not, however, provide dynamic information on the intracellular behavior of the targeted molecules. The aim of this work was to investigate ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.3464

    authors： Paillasson S,Van De Corput M,Dirks RW,Tanke HJ,Robert-Nicoud M,Ronot X

    更新日期：1997-02-25 00:00:00

  • Differential regulation of thyrotropin receptor and thyroglobulin mRNA accumulation at the cellular level: an in situ hybridization study.

    abstract：:Regulation of TSH receptor (TSHr) mRNA accumulation has been investigated in canine thyrocytes in primary culture by in situ hybridization experiments; the effects of the mitogenic thyrotropin (TSH), epidermal growth factor (EGF), and phorbol ester TPA (12-O-tetradecanoylphorbol-13-acetate) have been compared. Apart f...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90451-d

    authors： Pohl V,Maenhaut C,Gérard C,Vassart G,Dumont JE

    更新日期：1992-04-01 00:00:00

  • Polo-like kinase 3 is Golgi localized and involved in regulating Golgi fragmentation during the cell cycle.

    abstract：:The Golgi apparatus undergoes extensive fragmentation during mitosis in animal cells. Protein kinases play a critical role during fragmentation of the Golgi apparatus. We reported here that Polo-like kinase 3 (Plk3) may be an important mediator during Golgi breakdown. Specifically, Plk3 was concentrated at the Golgi a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2003.10.022

    authors： Ruan Q,Wang Q,Xie S,Fang Y,Darzynkiewicz Z,Guan K,Jhanwar-Uniyal M,Dai W

    更新日期：2004-03-10 00:00:00

  • Conjugation in the ciliate Euplotes octocarinatus: comparison of ciliary and cell body-associated glycoconjugates of non-mating-competent, mating-competent, and conjugating cells.

    abstract：:Pair formation in the hypotrichous ciliate Euplotes octocarinatus is a poorly understood phenomenon. In order to obtain information about the molecules involved in this process, we compared ciliary and cell body-associated glycoconjugates of non-mating-competent, mating-competent, and conjugating cells. Detection of g...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1114

    authors： Plümper E,Freiburg M,Heckmann K

    更新日期：1995-04-01 00:00:00

  • Osteoclast migration on phosphorylated osteopontin is regulated by endogenous tartrate-resistant acid phosphatase.

    abstract：:Osteopontin (OPN) is a multifunctional protein implicated in cellular adhesion and migration. Phosphorylation has emerged as a post-translational modification important for certain biological activities of OPN. This study demonstrates that adhesion of isolated neonatal rat osteoclasts in vitro was augmented on bovine ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.10.019

    authors： Ek-Rylander B,Andersson G

    更新日期：2010-02-01 00:00:00

  • Repopulation of a human alveolar matrix by adult rat type II pneumocytes in vitro. A novel system for type II pneumocyte culture.

    abstract：:This paper describes the preparation of lung acellular alveolar matrix fragments and culture of rat type II pneumocytes directly on the alveolar epithelial basement membrane, thereby permitting study of the effect of lung basement membrane on the morphology and function of type II cells. Collagen types I, III, IV and ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90347-2

    authors： Lwebuga-Mukasa JS,Ingbar DH,Madri JA

    更新日期：1986-02-01 00:00:00

  • Inhibition of mitosis in fertilized sea urchin eggs by inhibition of the cyclic AMP-dependent protein kinase.

    abstract：:Inhibition of cAMP-dependent protein kinase activity by microinjection of a specific physiologic protein inhibitor into sea urchin eggs inhibits the first cleavage after fertilization. Inhibition apparently occurs at some time prior to or during formation of the mitotic spindle. Measurement of the total protein kinase...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90286-j

    authors： Browne CL,Bower WA,Palazzo RE,Rebhun LI

    更新日期：1990-05-01 00:00:00

  • Nonmuscle myosin IIB, a sarcomeric component in the extraocular muscles.

    abstract：:Extraocular muscles (EOMs) are categorized as skeletal muscles; however, emerging evidence indicates that their gene expression profile, metabolic characteristics and functional properties are significantly different from the prototypical members of this muscle class. Gene expression profiling of developing and adult ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.03.018

    authors： Moncman CL,Andrade FH

    更新日期：2010-07-15 00:00:00

  • Isoforms of p38MAPK gamma and delta contribute to differentiation of human AML cells induced by 1,25-dihydroxyvitamin D₃.

    abstract：:Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.08.010

    authors： Zhang J,Harrison JS,Studzinski GP

    更新日期：2011-01-01 00:00:00

  • Altered permeability and modulatory character of connexin channels during mammary gland development.

    abstract：:Abrupt developmental changes occur in structural form and function of connexin (Cx) channels in the mouse mammary gland. Microarray study shows that the principal connexin isoform in epithelial cells during pregnancy is Cx26, up-regulated and persisting from the virgin. After parturition, there is rapid induction of C...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.05.003

    authors： Locke D,Stein T,Davies C,Morris J,Harris AL,Evans WH,Monaghan P,Gusterson B

    更新日期：2004-08-15 00:00:00

  • Tumor exosomes induce tunneling nanotubes in lipid raft-enriched regions of human mesothelioma cells.

    abstract：:Tunneling nanotubes (TnTs) are long, non-adherent, actin-based cellular extensions that act as conduits for transport of cellular cargo between connected cells. The mechanisms of nanotube formation and the effects of the tumor microenvironment and cellular signals on TnT formation are unknown. In the present study, we...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.01.014

    authors： Thayanithy V,Babatunde V,Dickson EL,Wong P,Oh S,Ke X,Barlas A,Fujisawa S,Romin Y,Moreira AL,Downey RJ,Steer CJ,Subramanian S,Manova-Todorova K,Moore MAS,Lou E

    更新日期：2014-04-15 00:00:00

  • Identification of integrins involved in cell adhesion to native and denatured type I collagens and the phenotypic transition of rabbit arterial smooth muscle cells.

    abstract：:Rabbit smooth muscle cells (SMC) in primary culture attached to and started proliferating on native and heat-denatured type I collagens, although the amount of cell attachment to denatured collagen was significantly lower. The cells adhered poorly and were unable to grow on commercial gelatin. In contrast, synthetic S...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1225

    authors： Yamamoto M,Yamato M,Aoyagi M,Yamamoto K

    更新日期：1995-07-01 00:00:00

  • Permeability and metabolic properties of a trophoblast cell line (HRP-1) derived from normal rat placenta.

    abstract：:The HRP-1 cell line is derived from normal rat placenta and appears morphologically similar to and retains characteristic expression of cellular markers of labyrinthine trophoblast cells. In this study, monolayers of HRP-1 cells grown on permeable supports were evaluated as a potential in vitro system to study trophob...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3603

    authors： Shi F,Soares MJ,Avery M,Liu F,Zhang X,Audus KL

    更新日期：1997-07-10 00:00:00

  • Functional conservation of 14-3-3 isoforms in inhibiting bad-induced apoptosis.

    abstract：:14-3-3 proteins are a family of homologous eukaryotic molecules with seven distinct isoforms in mammalian cells. Isoforms of 14-3-3 proteins interact with diverse ligands and are involved in the regulation of mitogenesis, cell cycle progression, and apoptosis. However, whether different 14-3-3 isoforms are responsible...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5376

    authors： Subramanian RR,Masters SC,Zhang H,Fu H

    更新日期：2001-11-15 00:00:00

  • Reserpine-induced alterations in mucus production and calmodulin-binding proteins in a human epithelial cell line.

    abstract：:The characterization of a mucus-producing human cell line (HC-84) derived from a colon carcinoma and its response to in vitro reserpine treatment is reported. Mucous granules were demonstrated within these cells on the basis of electron microscopic examination and incorporation of [3H]glucosamine with subsequent autor...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90709-2

    authors： Brady RC,Karnaky KJ Jr,Dedman JR

    更新日期：1984-01-01 00:00:00

  • Activation of the gene for atrial natriuretic factor during in vitro cardiac myogenesis by P19 embryonal carcinoma cells.

    abstract：:We examined the transcriptional activity profile of the gene for atrial natriuretic factor (ANF) in mouse embryonal carcinoma P19 cells which had been induced for in vitro cardiac myogenesis. Differentiation was assessed visually, by the degree of spontaneous beating activity, and by the appearance of striated muscle ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1210

    authors： Boer PH

    更新日期：1993-08-01 00:00:00

  • Cytoskeletal binding proteins distinguish cultured dental follicle cells and periodontal ligament cells.

    abstract：:Human dental follicle cells (DFCs) and periodontal ligament cells (PDLCs) derived from the ectomesenchymal tissue, have been shown to exhibit stem/progenitor cell properties and the ability to induce tissue regeneration. Stem cells in dental follicle differentiate into cementoblasts, periodontal ligament fibroblasts a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.12.011

    authors： Li J,Li H,Tian Y,Yang Y,Chen G,Guo W,Tian W

    更新日期：2016-07-01 00:00:00

  • Head-to-head association in bovine spermatozoa induced by catecholamines.

    abstract：:Noradrenaline, adrenaline, and isoproterenol induce head-to-head association in bovine spermatozoa in a Tris-HCl-buffered medium. Noradrenaline was the most and isoproterenol the least efficient. This effect was stimulated by Ca2+, Mg2+ and Mn2+, Ca2+ being more efficient than the other two ions. At 2 X 10(-6) M Ca2+,...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(78)90384-1

    authors： Lindahl PE

    更新日期：1978-05-01 00:00:00

  • Viscoelasticity in wild-type and vinculin-deficient (5.51) mouse F9 embryonic carcinoma cells examined by atomic force microscopy and rheology.

    abstract：:We have been studying mouse F9 embryonic carcinoma cells which contain no detectable vinculin protein (5.51 cells), and compared them with F9 wild-type cells. Employing atomic force microscopy, we probed the elastic properties of individual F9 wild-type and 5.51 cells by measuring the dynamic response of controlled lo...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0223

    authors： Goldmann WH,Ezzell RM

    更新日期：1996-07-10 00:00:00

  • The protein tyrosine phosphatase SHP-2 is required for EGFRvIII oncogenic transformation in human glioblastoma cells.

    abstract：:Oncogenic EGFRvIII is a naturally occurring oncoprotein and is expressed in about 40-50% of human glioblastomas, particularly those that arise de novo. To understand the molecular mechanisms by which this oncoprotein alters transforming phenotypes, and since our previous work indicated that SHP-2 protein tyrosine phos...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.05.001

    authors： Zhan Y,Counelis GJ,O'Rourke DM

    更新日期：2009-08-15 00:00:00