Abstract:
:Rabbit smooth muscle cells (SMC) in primary culture attached to and started proliferating on native and heat-denatured type I collagens, although the amount of cell attachment to denatured collagen was significantly lower. The cells adhered poorly and were unable to grow on commercial gelatin. In contrast, synthetic SMC in secondary culture could adhere to gelatin and grew as well on gelatin as on native type I collagen. The SMC in the contractile state adhered to native type I collagen through the alpha 1 beta 1 and alpha 3 beta 1 integrins. The cells in the intermediate phenotype also adhered to the substrate through the alpha 1 beta 1 and alpha 3 beta 1 integrins, but the relative amount of alpha 3 integrin decreased. The initial adhesion of cells in secondary culture to native type I collagen was mediated only by the alpha 1 beta 1 integrin. The cell-binding sequences did not contain DGEA (Asp-Gly-Glu-Ala) or RGD (Arg-Gly-Asp). In contrast, cell adhesion to heat-denatured type I collagen was mediated only by the alpha 1 beta 1 integrin in the contractile state and by the alpha 1 beta 1, alpha 2 beta 1, and alpha 3 beta 1 integrins in the synthetic state. In heat-denatured type I collagen, the sequences DGEA and RGD served as a recognition site for the alpha 2 beta 1 and alpha 3 beta 1 integrins. Our results suggest that rabbit SMC can recognize the native and denatured type I collagens through interactions with the triple helix-binding receptors and alpha chain-binding receptors and that the expression pattern of integrins changes in conjunction with the phenotypic properties of vascular SMC.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Yamamoto M,Yamato M,Aoyagi M,Yamamoto Kdoi
10.1006/excr.1995.1225subject
Has Abstractpub_date
1995-07-01 00:00:00pages
249-56issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(85)71225-6journal_volume
219pub_type
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