Abstract:
:SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) were found to contain an additional protein of 52.5 kD molecular weight (MW). This protein was identified by its reactivity with the monoclonal antibody TROMA-I as being keratin no. 8, a keratin normally present only in simple epithelia. Since this keratin is present in fetal epidermis but disappears gradually when fetal skin becomes multilayered after week 13 of development (Moll et al., Differentiation 23 (1982) 170. [23]), it suggests that SV40 virus and TPA are able to induce in human keratinocytes the reexpression of fetal characters.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Darmon M,Delescluse C,Semat A,Bernard B,Bailly J,Prunieras Mdoi
10.1016/0014-4827(84)90692-xsubject
Has Abstractpub_date
1984-09-01 00:00:00pages
315-9issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(84)90692-Xjournal_volume
154pub_type
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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