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Identification of the type-B receptor for platelet-derived growth factor in human embryonal carcinoma cells.

Abstract：

:The human embryonal carcinoma (EC) cell line Tera 2 clone 13 (T2/13) can be induced to differentiate in vitro into neuroectodermal cell types with retinoic acid. Undifferentiated cells are characterized by rapid proliferation, whereas differentiated cells show a prolonged generation time, have a limited life span, and possess new cell-surface markers. In the present study we establish that both differentiated and undifferentiated T2/13 cells express the type-B platelet-derived growth factor (PDGF) receptor mRNA and bind PDGF-BB with high affinity. Differentiation causes a three-fold increase in receptor number per cell and leaves the affinity of the receptors unaffected. These data are the first to describe expression of this receptor in EC cells. The biosynthesis and degradation of this receptor were studied in undifferentiated as well as in differentiated T2/13 cells using an anti-type-B receptor antibody. These experiments revealed that high concentrations of recombinant PDGF-AA did not accelerate receptor metabolism in both cell types. In contrast, human PDGF or recombinant PDGF-BB added to the culture dishes readily increased receptor degradation. These results demonstrate that T2/13 cells express functional type-B PDGF receptors and suggest that cells responsive to PDGF might be present during mammalian development before the onset of mesoderm formation.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Weima SM,van Rooijen MA,Mummery CL,Feyen A,de Laat SW,van Zoelen EJ

doi

10.1016/0014-4827(90)90312-x

subject

Has Abstract

pub_date

1990-02-01 00:00:00

pages

324-31

issue

2

eissn

0014-4827

issn

1090-2422

journal_volume

186

pub_type

杂志文章

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