Hypomethylation of human heterochromatin detected by restriction enzyme nick translation.

abstract：BACKGROUND:Functional activation of oncogenic K-Ras signaling pathway plays an important role in the early events of colorectal carcinogenesis (CRC). K-Ras proto-oncogene is involved in 35-40% of CRC cases. Mutations in the Ras gene trigger the transduction of proliferative and anti-apoptotic signals, even in the absen...

journal_title：Experimental cell research

authors： Naumov I,Kazanov D,Lisiansky V,Starr A,Aroch I,Shapira S,Kraus S,Arber N

更新日期：2012-01-15 00:00:00

abstract：:By a sequential mutation and selection utilizing N-methyl-N'-nitro-N-nitrosoguanidine as a mutagen, we succeeded in separating a poly(ADP ribose) polymerase-defective mutant clone (Cl-3527) from a mouse L1210 cell clone (Cl-3). The enzyme activity per cell in Cl-3527 cells was only 8% of that in wild type L1210 (CCL 2...

journal_title：Experimental cell research

authors： Yoshihara K,Itaya A,Hironaka T,Sakuramoto S,Tanaka Y,Tsuyuki M,Inada Y,Kamiya T,Ohnishi K,Honma M

更新日期：1992-05-01 00:00:00

abstract：:Keratinocytes in human skin undergo apoptosis during various inflammatory processes and after ultraviolet (UV) irradiation. To determine if keratinocyte apoptosis may be mediated by the Fas/APO-1 receptor (CD95), a signal transduction pathway known to initiate programmed cell death of lymphocytes, we investigated Fas ...

journal_title：Experimental cell research

authors： Leverkus M,Yaar M,Gilchrest BA

更新日期：1997-05-01 00:00:00

abstract：:Screening for microbial metabolites that induce transcriptional activation of the SV40 promoter resulted in the identification of two known compounds, FR901228 and trichostatin A (TSA). FR901228 is a potent antitumor drug that is currently under clinical investigation. TSA is a specific inhibitor of histone deacetylas...

journal_title：Experimental cell research

authors： Nakajima H,Kim YB,Terano H,Yoshida M,Horinouchi S

更新日期：1998-05-25 00:00:00

abstract：:Bax-mediated apoptosis in neurons is involved in many pathologic conditions affecting the central nervous system, including degenerative diseases, stroke, and trauma. Two molecules belonging to the Bcl-2 family, Bcl-2 and Bcl-X(L), protect cells from Bax-induced apoptosis and show distinct expression patterns in adult...

journal_title：Experimental cell research

authors： Shinoura N,Satou R,Yoshida Y,Asai A,Kirino T,Hamada H

更新日期：2000-02-01 00:00:00

abstract：:By means of plasma clot clonal cultures, the content of the avian spleen in granulomonocytic progenitors was studied during ontogeny. Serum-free media were used that were supplemented with growth activities produced either by embryonic fibroblasts or adult spleen cells. These two conditioned media not only permitted t...

journal_title：Experimental cell research

authors： Nicolas-Bolnet C,Yassine F,Cormier F,Dieterlen-Lièvre F

更新日期：1991-10-01 00:00:00

abstract：:Implantation of myoblasts is a strategy used to enhance the regeneration of skeletal muscle tissue in vivo. In mouse models, myogenic cell lines and primary cells have been employed with different yields of adult muscle tissue formed. The present work is a study of some developmental features of expanded primary mouse...

journal_title：Experimental cell research

authors： Lorenzon P,Bernareggi A,Degasperi V,Nurowska E,Wernig A,Ruzzier F

更新日期：2002-08-01 00:00:00

abstract：:It has been suggested that DNA organized into replication foci during S-phase remains stably aggregated in non-S-phase cells and that these stable aggregates provide fundamental units of nuclear or chromosome architecture [C. Meng and R. Berezney (1991) J. Cell Biol. 115, 95a; E. Sparvoli et al. (1994) J. Cell Sci. 10...

journal_title：Experimental cell research

authors： Zink D,Bornfleth H,Visser A,Cremer C,Cremer T

更新日期：1999-02-25 00:00:00

abstract：:Cells with and without hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity were used to examine the transfer of purine metabolites through the medium and via cell contacts. HGPRT- Chinese hamster and human fibroblasts were able to incorporate 3H-labeled purine metabolite(s) from medium in which mouse HGPRT...

journal_title：Experimental cell research

authors： Bols NC,Mosser DD,Boliska SA

更新日期：1987-11-01 00:00:00

abstract：:Annexin 2 is a Ca(2+)-dependent phospholipid-binding protein that is involved in secretion. Despite the fact that this protein does not have signals for its secretion, many reports have shown its presence in the extracellular milieu. Here we demonstrate that, upon stimulation of exocytosis in chromaffin cells, a fract...

journal_title：Experimental cell research

authors： Faure AV,Migné C,Devilliers G,Ayala-Sanmartin J

更新日期：2002-05-15 00:00:00

abstract：:Insulin-like growth factor-1 (IGF-1) and its receptor (IGF-1R) are required for cell proliferation in vitro, raising the possibility that an autocrine IGF-1-IGF-1R system may be present in vivo and become activated in the viable ventricular myocytes shortly after infarction. Therefore, following the in vivo documentat...

journal_title：Experimental cell research

authors： Reiss K,Kajstura J,Zhang X,Li P,Szoke E,Olivetti G,Anversa P

更新日期：1994-08-01 00:00:00

abstract：:When exponentially growing NHIK 3025 cells were shifted from medium containing 30% serum to medium containing 0.03% serum the rate of net protein accumulation was reduced due to both a reduction in the rate of protein synthesis and an increase in the rate of protein degradation. This change in growth conditions increa...

journal_title：Experimental cell research

authors： Rønning OW,Pettersen EO

更新日期：1984-11-01 00:00:00

abstract：:The phosphorylation state of the CD18-chain of beta 2-integrins have been shown not to mediate changes in the avidity of these receptors (i.e., inside-out signaling); however, no alternative functional significance has been proposed. Our study focused on how changes in the phosphorylation state of beta 2-integrin-rece...

journal_title：Experimental cell research

authors： Hellberg C,Eierman D,Sjölander A,Andersson T

更新日期：1995-03-01 00:00:00

abstract：:The Ras-like family of small GTPases includes, among others, Ras, Rap1, R-ras, and Ral. The family is characterized by similarities in the effector domain. While the function of Ras is, at least in part, elucidated, little is known about other members of the family. Currently, much attention is focused on the small GT...

journal_title：Experimental cell research

authors： Zwartkruis FJ,Bos JL

更新日期：1999-11-25 00:00:00

abstract：:A sufficient supply molecular oxygen is essential for the maintenance of physiologic metabolism and bioenergetic homeostasis for most metazoans. For this reason, mechanisms have evolved for eukaryotic cells to adapt to conditions where oxygen demand exceeds supply (hypoxia). These mechanisms rely on the modification o...

journal_title：Experimental cell research

authors： Cavadas MAS,Cheong A,Taylor CT

更新日期：2017-07-15 00:00:00

abstract：:The male fetal lung begins to synthesize surfactant later in gestation than the female. This delay appears to be caused by androgens. We hypothesized that male fetal lung differentiation is delayed as a consequence of an extended phase of growth which is elicited by androgens. We observed that in vivo fetal lung prote...

journal_title：Experimental cell research

authors： Nielsen HC,Kirk WO,Sweezey N,Torday JS

更新日期：1990-05-01 00:00:00

abstract：:In addition to the well-characterized surface gap junctions expressed at contact sites between cells, annular gap junction profiles have been localized within the cytoplasm of some cell populations. To study and characterize these annular profiles, gap junction protein type was demonstrated with Western blot and immun...

journal_title：Experimental cell research

authors： Murray SA,Williams SY,Dillard CY,Narayanan SK,McCauley J

更新日期：1997-08-01 00:00:00

abstract：:Polyamines affect nucleosome oligomerization and DNA conformation in vitro, yet little information exists regarding the influence of naturally synthesized polyamines on mammalian chromatin. Capitalizing on the relative inefficiency of a moderate ionic strength extraction buffer to dissociate histones, we obtained evid...

journal_title：Experimental cell research

authors： Hobbs CA,Paul BA,Gilmour SK

更新日期：2003-11-01 00:00:00

abstract：:The interaction of fluorescein-labeled nerve growth factor (NGF) with human melanoma cells (A875) has been studied in order to assess better methodology for rigorous NGF binding studies. The NGF was modified at a single carboxyl group with iodoacetamidofluorescein after reaction with carbodiimide and cystamine. The mo...

journal_title：Experimental cell research

authors： Kasaian MT,Jacobberger JW,Neet KE

更新日期：1994-01-01 00:00:00

abstract：:Lysosomal cysteine proteinase cathepsin B is implicated in remodeling the extracellular matrix, a crucial step in the process of tumor cell invasion. In this study the contributions of intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT,...

journal_title：Experimental cell research

authors： Premzl A,Zavasnik-Bergant V,Turk V,Kos J

更新日期：2003-02-15 00:00:00

abstract：:Members of the Bcl-2 family of proteins are key regulators of apoptosis. Some of these proteins undergo posttranslational modification, such as phosphorylation or proteolysis, that serves to alter their function. Caspases are known to cleave Bid, a proapoptotic family member, as well as Bcl-2 and Bcl-X(L), two prosurv...

journal_title：Experimental cell research

authors： Wood DE,Newcomb EW

更新日期：2000-05-01 00:00:00

abstract：:This review article describes the pathways and mechanisms of endocytosis and post-endocytic sorting of the EGF receptor (EGFR/ErbB1) and other members of the ErbB family. Growth factor binding to EGFR accelerates its internalization through clathrin-coated pits which is followed by the efficient lysosomal targeting of...

journal_title：Experimental cell research

authors： Sorkin A,Goh LK

更新日期：2009-02-15 00:00:00

abstract：:Embryonic stem (ES) cells have been tested for potential cell transplantation therapy for CNS disorders. Understanding their differentiation mechanism and identifying factors involved in driving excitatory and inhibitory neuron lineages should enhance the efficacy and efficiency of the cell transplantation therapy. We...

journal_title：Experimental cell research

authors： Theus MH,Wei L,Francis K,Yu SP

更新日期：2006-10-01 00:00:00

abstract：:We have shown previously that there is a good correlation between the degree of microtubule disassembly by methylmercury (MeHg) and the extent of inhibition of DNA replication in Concanavalin A (Con A)-stimulated mouse splenic lymphocytes. The purpose of this study was to determine if these two events are causally rel...

journal_title：Experimental cell research

authors： Roy C,Prasad KV,Reuhl KR,Little JE,Valentine BK,Brown DL

更新日期：1991-08-01 00:00:00

abstract：:The correct functioning of epidermal growth factor receptor (EGFR), a receptor tyrosine kinase, is required for normal skin development and homeostasis. Cellular hyperproliferation induced by dysregulation of EGFR is tightly associated with structural and functional defects of hair follicles, skin lesions, and tumorig...

journal_title：Experimental cell research

authors： Chen L,Wang T,Ji X,Ding C,Liang T,Liu X,Lu J,Guo X,Kang Q,Ji Z

更新日期：2019-11-15 00:00:00

abstract：:Ovarian cancer (OC) is the leading cause of death in gynecologic diseases in which there is evidence for a complex chemokine network. Chemokines are a family of proteins that play an important role in tumor progression influencing cell proliferation, angiogenic/angiostatic processes, cell migration and metastasis, and...

journal_title：Experimental cell research

authors： Porcile C,Bajetto A,Barbieri F,Barbero S,Bonavia R,Biglieri M,Pirani P,Florio T,Schettini G

更新日期：2005-08-15 00:00:00

abstract：:BMPs and Hox proteins play crucial roles in developmental processes. Beyond their mutual regulation of gene expression, little is known about the relations between their mechanisms of actions. Previously, we have shown that Hoxc8 acts as a downstream repressor in the BMP signaling pathway. Smad1 and Smad6 interact wit...

journal_title：Experimental cell research

authors： Li X,Nie S,Chang C,Qiu T,Cao X

更新日期：2006-04-01 00:00:00

abstract：:In the past, a unique type of precursor for a secretory protein was discovered. It contains a central repetitive domain rich in threonine residues and terminal cysteine-rich domains. Due to striking homologies of these terminal domains with pancreatic spasmolytic polypeptide, originally the name "prepro-spasmolysin" w...

journal_title：Experimental cell research

authors： Hauser F,Gertzen EM,Hoffmann W

更新日期：1990-08-01 00:00:00

abstract：:The actin interaction of coronin 3 has been mainly documented by in vitro experiments. Here, we discuss coronin 3 properties in the light of new structural information and focus on assays that reflect in vivo roles of coronin 3 and its impact on F-actin-associated functions. Using GFP-tagged coronin 3 fusion proteins ...

journal_title：Experimental cell research

authors： Rosentreter A,Hofmann A,Xavier CP,Stumpf M,Noegel AA,Clemen CS

更新日期：2007-03-10 00:00:00

abstract：:To study delayed genetic and epigenetic radiation effects, which may trigger radiation-induced carcinogenesis, we have established single-cell clones from irradiated and non-irradiated primary human fibroblasts. Stable clones were endowed with the same karyotype in all analyzed metaphases after 20 population doublings...

journal_title：Experimental cell research

authors： Flunkert J,Maierhofer A,Dittrich M,Müller T,Horvath S,Nanda I,Haaf T

更新日期：2018-09-15 00:00:00