Abstract:
:A portion of the DNA within intact nuclei of a spontaneously transformed Chinese hamster ovary cell line (CHO-Kl) is relatively resistant to digestion by pancreatic deoxyribonuclease, as compared to nuclei from primary cultures of Chinese hamster ovary fibroblasts. Treatment of CHO-Kl cells with derivatives of 3',5' cyclic AMP (cAMP) under conditions which effect the reverse transformation (RT) of these cells, results in restoration of the increased sensitivity of their DNA to hydrolysis by pancreatic deoxyribonuclease, to the level characteristic of an untransformed, morphologically normal Chinese hamster fibroblast cell line. Dibutyryl (db-)cAMP and 8-bromo-cAMP (Br-cAMP) yielded similar results. The cAMP derivatives employed had no effect on the normal fibroblasts.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Schonberg S,Patterson D,Puck TTdoi
10.1016/s0014-4827(83)80007-xsubject
Has Abstractpub_date
1983-04-15 00:00:00pages
57-62issue
1eissn
0014-4827issn
1090-2422journal_volume
145pub_type
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更新日期:1984-09-01 00:00:00
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