Abstract：
BACKGROUND:CDK8 is associated with the transcriptional Mediator complex and has been shown to regulate several transcription factors implicated in cancer. As a pancreatic cancer oncogene, the role of CDK8 in cancer angiogenesis remains unclear. Here, we investigated the contribution of CDK8 in pancreatic cancer angiogenesis and examined the underlying molecular mechanisms. METHODS:CDK8 expression was evaluated via immunohistochemistry, western blotting, and qRT-PCR in relation to the clinicopathological characteristics of pancreatic cancer patients. The effects of silencing or overexpressing CDK8 on cancer angiogenesis were assessed in vitro by western blotting assays in pancreatic cancer cell lines and in vivo with nude mice xenograft models. RESULTS:Compared with adjacent normal tissues, pancreatic cancer tissues showed upregulation of CDK8 expression, which was inversely correlated with T grade, liver metastasis, size, lymph node metastasis and poor survival. CDK8 overexpression promoted angiogenesis in pancreatic cancer via activation of the CDK8-β-catenin-KLF2 signaling axis, as demonstrated by the upregulation and downregulation of signals representing the rate-limiting steps in angiogenesis. Silencing CDK8 inhibited angiogenesis in pancreatic cancer in vitro. Additionally, these results were confirmed in nude mice xenograft models in vivo. CONCLUSIONS:CDK8 promotes angiogenesis in pancreatic cancer via activation of the CDK8-β-catenin-KLF2 signaling axis, thus providing valid targets for the treatment of pancreatic cancer.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Wei R,Kong L,Xiao Y,Yuan H,Song Y,Wang J,Yu H,Mao S,Xu Wdoi
10.1016/j.yexcr.2018.05.033subject
Has Abstractpub_date
2018-08-15 00:00:00pages
304-315issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(18)30304-5journal_volume
369pub_type
杂志文章abstract：:Regulatory B cells (Breg cells) play critical roles in modulating immune responses during autoimmune diseases and infection. Here we explored the participation of two main Breg subsets, including IL-10+ Breg (B10) and IL-35+ Breg cells, in maintaining successful pregnancy. We first observed an elevated percentage of B...
journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.4943
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3930
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1247
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abstract：:The effect of SK&F 96365 (1-(beta-[3-(4-methoxyphenyl)propoxyl]-4- methoxyphenethyl)-1H-imidazole hydrochloride), a recently synthesized inhibitor of receptor-mediated calcium entry, was investigated on human hematopoietic cell lines. We found that treatment of the T-cell leukemia line Jurkat with SK&F 96365 inhibited...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90103-f
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90346-6
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90373-2
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90701-8
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90265-0
更新日期：1988-03-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.1998.4278
更新日期：1999-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2019.01.005
更新日期：2019-03-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3831
更新日期：1998-01-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90482-9
更新日期：1986-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.07.029
更新日期：2006-11-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2016.07.025
更新日期：2016-08-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1391
更新日期：1995-12-01 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2016-02-15 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2009.09.026
更新日期：2009-12-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3655
更新日期：1997-08-25 00:00:00
abstract：:Ring finger protein 6 (RNF6), a RING-domain E3 ubiquitin Ligase, functions as a potential tumor promoter in several caners. However, the function and clinical significance of RNF6 in hepatocellular carcinoma (HCC) remains unclear. Here, we found that high RNF6 expression was detected frequently in primary HCC tissues,...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.11.019
更新日期：2019-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5576
更新日期：2002-09-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.04.008
更新日期：2005-08-01 00:00:00
abstract：:In order to investigate the molecular mechanism of biological action of the drug cyclosporin A (CsA), we have studied its effect on eukaryotic RNA polymerase activities in two systems: (a) in vitro transcription; (b) injection into frog oocytes. The electron microscopic transcription R-loop method [1] has been used to...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90382-3
更新日期：1984-04-01 00:00:00
