Xenopus single-minded (xSim) is a nuclear factor allowing nuclear translocation of its cytoplasmic partner xArnt.

Abstract:

:Transcription factors belonging to the basic helix-loop-helix Per-Arnt-Sim (bHLH/PAS) family control a wide variety of biological processes in mammalian and/or Drosophila. We have previously isolated bHLH/PAS Xenopus amphibian homologs of Single-minded (xSim) and aryl receptor nuclear translocator (xArnt) and characterized their expression pattern during embryogenesis. We show in this paper that xSim protein is a functional homolog of Drosophila or mammalian Sim(s). Biochemical analysis indicates that xSim forms a heterodimer with xArnt. Subcellular localization analysis of bHLH/PAS chimeric fluorescent versions in Xenopus or mammalian cell lines shows that xSim is constitutively localized in the nuclear compartment. On the opposite, xArnt appears to be predominantly expressed in the cytoplasm. In addition, we demonstrate that xArnt nuclear localization depends on the presence of xSim. Thus xSim appears to be an essential factor in the nuclear translocation of the xSim/xArnt complex. In perfect agreement, we show that the C-terminal half of xSim contains the information for this nuclear localization.

journal_name

Exp Cell Res

authors

Coumailleau P,Bollérot K,Lecomte C,Angelier N

doi

10.1016/s0014-4827(03)00051-x

keywords:

subject

Has Abstract

pub_date

2003-07-15 00:00:00

pages

237-48

issue

2

eissn

0014-4827

issn

1090-2422

pii

S001448270300051X

journal_volume

287

pub_type

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