当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > The MAPK ERK5, but not ERK1/2, inhibits the progression of monocytic phenotype to the functioning macrophage.

The MAPK ERK5, but not ERK1/2, inhibits the progression of monocytic phenotype to the functioning macrophage.

Abstract：

:Intracellular signaling pathways present targets for pharmacological agents with potential for treatment of neoplastic diseases, with some disease remissions already recorded. However, cellular compensatory mechanisms usually negate the initial success. For instance, attempts to interrupt aberrant signaling downstream of the frequently mutated ras by inhibiting ERK1/2 has shown only limited usefulness for cancer therapy. Here, we examined how ERK5, that overlaps the functions of ERK1/2 in cell proliferation and survival, functions in a manner distinct from ERK1/2 in human AML cells induced to differentiate by 1,25D-dihydroxyvitamin D3 (1,25D). Using inhibitors of ERK1/2 and of MEK5/ERK5 at concentrations specific for each kinase in HL60 and U937 cells, we observed that selective inhibition of the kinase activity of ERK5, but not of ERK1/2, in the presence of 1,25D resulted in macrophage-like cell morphology and enhancement of phagocytic activity. Importantly, this was associated with increased expression of the macrophage colony stimulating factor receptor (M-CSFR), but was not seen when M-CSFR expression was knocked down. Interestingly, inhibition of ERK1/2 led to activation of ERK5 in these cells. Our results support the hypothesis that ERK5 negatively regulates the expression of M-CSFR, and thus has a restraining function on macrophage differentiation. The addition of pharmacological inhibitors of ERK5 may influence trials of differentiation therapy of AML.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Wang X,Pesakhov S,Harrison JS,Kafka M,Danilenko M,Studzinski GP

doi

10.1016/j.yexcr.2014.10.003

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

199-211

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(14)00442-X

journal_volume

330

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Endothelial monocyte activating polypeptide-II modulates endothelial cell responses by degrading hypoxia-inducible factor-1alpha through interaction with PSMA7, a component of the proteasome.

    abstract：:The majority of human tumors are angiogenesis dependent. Understanding the specific mechanisms that contribute to angiogenesis may offer the best approach to develop therapies to inhibit angiogenesis in cancer. Endothelial monocyte activating polypeptide-II (EMAP-II) is an anti-angiogenic cytokine with potent effects ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.03.021

    authors： Tandle AT,Calvani M,Uranchimeg B,Zahavi D,Melillo G,Libutti SK

    更新日期：2009-07-01 00:00:00

  • Organ and class specificity of cell adhesion blocking antisera.

    abstract：:Rabbit antisera prepared against embryonic chick liver cells have been characterized using cell cytotoxicity tests, antibody absorption tests, inhibition of cell-cell reaggregation and cell-aggregate adhesion assays. The sera contain antibodies which specifically block embryonic chick liver cell-cell aggregation with ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(82)90420-7

    authors： Grady SR,Nielsen LD,McGuire EJ

    更新日期：1982-11-01 00:00:00

  • A functional role for ERK in gene induction, but not in neurite outgrowth in differentiating neuroblastoma cells.

    abstract：:The human neuroblastoma cell line SH-SY5Y can differentiate into a functional sympathetic neuronal phenotype when treated with low concentrations of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in the presence of serum or defined growth factors. When TrkA is introduced into the cells, NGF also induces ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5163

    authors： Olsson AK,Nånberg E

    更新日期：2001-04-15 00:00:00

  • Migration potential and gene expression profile of human mesenchymal stem cells induced by CCL25.

    abstract：:Recruitment of mesenchymal stem cells (MSC) to tissue damages is a promising approach for in situ tissue regeneration. The physiological mechanisms and regulatory processes of MSC trafficking to injured tissue remain poorly understood. However, the pivotal role of chemokines in MSC recruitment has already been shown. ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.12.022

    authors： Binger T,Stich S,Andreas K,Kaps C,Sezer O,Notter M,Sittinger M,Ringe J

    更新日期：2009-05-01 00:00:00

  • Towards a further understanding of the growth-inhibiting action of oxygen deficiency. Evaluation of the effect of antimycin on proliferating Ehrlich ascites tumour cells.

    abstract：:The effect of 1 microM antimycin on the proliferative properties, metabolism and basic cell composition of Ehrlich ascites tumour cells cultured in the second in vitro passage was studied. Continuous drug exposure of asynchronous cells caused rapid cessation of cell growth, characterized by the cell number and DNA, RN...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90162-4

    authors： Löffler M

    更新日期：1985-03-01 00:00:00

  • FR901228, a potent antitumor antibiotic, is a novel histone deacetylase inhibitor.

    abstract：:Screening for microbial metabolites that induce transcriptional activation of the SV40 promoter resulted in the identification of two known compounds, FR901228 and trichostatin A (TSA). FR901228 is a potent antitumor drug that is currently under clinical investigation. TSA is a specific inhibitor of histone deacetylas...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4027

    authors： Nakajima H,Kim YB,Terano H,Yoshida M,Horinouchi S

    更新日期：1998-05-25 00:00:00

  • Dynamic organization of DNA replication in one-cell mouse embryos: relationship to transcriptional activation.

    abstract：:We have analyzed the spatial and temporal relationship between transcription and replication sites during the first cell cycle in mouse embryos. Embryos were microinjected with both 5-bromouridine-5'-triphosphate and digoxygenin-11-deoxyuridine-5'-triphosphate to visualize transcription and replication sites respectiv...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3708

    authors： Bouniol-Baly C,Nguyen E,Besombes D,Debey P

    更新日期：1997-10-10 00:00:00

  • Downregulation of the growth hormone-induced Janus kinase 2/signal transducer and activator of transcription 5 signaling pathway requires an intact actin cytoskeleton.

    abstract：:Transient activation of the signal transducers and activators of transcription (STAT) proteins in response to growth hormone (GH) and other type II cytokines plays a pivotal role on specific gene transcription. The negative regulation of STATs seems to be exerted at the GH receptor (GHR)/Janus Kinase (JAK) complex and...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2003.11.017

    authors： Rico-Bautista E,Negrín-Martínez C,Novoa-Mogollón J,Fernández-Perez L,Flores-Morales A

    更新日期：2004-03-10 00:00:00

  • Correlation between torsion and streaming in Physarum.

    abstract：:Experiments are described which show that the torsional oscillations arising in a strand of the slime mould, Physarum polycephalum, under certain conditions remain closely correlated to oscillations in cytoplasmic streaming. When this is the case, the direction of rotation is related to the direction of streaming by a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90401-9

    authors： Obendorf P,O'Sullivan RA

    更新日期：1983-11-01 00:00:00

  • A basic peptide within the juxtamembrane region is required for EGF receptor dimerization.

    abstract：:The epidermal growth factor receptor (EGFR) is fundamental for normal cell growth and organ development, but has also been implicated in various pathologies, notably tumors of epithelial origin. We have previously shown that the initial 13 amino acids (P13) within the intracellular juxtamembrane region (R645-R657) are...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.08.032

    authors： Aifa S,Aydin J,Nordvall G,Lundström I,Svensson SP,Hermanson O

    更新日期：2005-01-01 00:00:00

  • Thrombin-mast cell interactions. Binding and cell activation.

    abstract：:Activation of mouse bone marrow-derived mast cells (BMMC) by thrombin (0.05-0.5 U/million cells) resulted in a concentration-dependent release of histamine, which levelled off by 0.1 U thrombin. Rat peritoneal mast cells (RMC) were not stimulated by thrombin, though in control experiments, both types of mast cells deg...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90184-3

    authors： Razin E,Baranes D,Marx G

    更新日期：1985-10-01 00:00:00

  • Analysis of the plasminogen system on rat glomerular epithelial cells.

    abstract：:Rat glomerular epithelial cells were grown to confluency on semipermeable tissue culture inserts and the plasminogen system of these cells was analyzed using enzyme assays, Western analysis, zymography, and reverse transcriptase-PCR. The glomerular epithelial cells are capable of activating exogenous plasminogen to pl...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1191

    authors： Kanalas JJ

    更新日期：1995-06-01 00:00:00

  • MT1-MMP initiates activation of pro-MMP-2 and integrin alphavbeta3 promotes maturation of MMP-2 in breast carcinoma cells.

    abstract：:We evaluated cellular mechanisms involved in the activation pathway of matrix prometalloproteinase-2 (pro-MMP-2), an enzyme implicated in the malignant progression of many tumor types. Membrane type-1 matrix metalloproteinase (MT1-MMP) cleaves the N-terminal prodomain of pro-MMP-2 thus generating the activation interm...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.5118

    authors： Deryugina EI,Ratnikov B,Monosov E,Postnova TI,DiScipio R,Smith JW,Strongin AY

    更新日期：2001-02-15 00:00:00

  • Cytoskeletal binding proteins distinguish cultured dental follicle cells and periodontal ligament cells.

    abstract：:Human dental follicle cells (DFCs) and periodontal ligament cells (PDLCs) derived from the ectomesenchymal tissue, have been shown to exhibit stem/progenitor cell properties and the ability to induce tissue regeneration. Stem cells in dental follicle differentiate into cementoblasts, periodontal ligament fibroblasts a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.12.011

    authors： Li J,Li H,Tian Y,Yang Y,Chen G,Guo W,Tian W

    更新日期：2016-07-01 00:00:00

  • Inhibition of ErbB2/neuregulin signaling augments paclitaxel-induced cardiotoxicity in adult ventricular myocytes.

    abstract：:Paclitaxel (Taxol) has been successfully combined with the monoclonal antibody trastuzumab (Herceptin) in the treatment of ErbB2 overexpressing cancers. However, this combination therapy showed an unexpected synergistic increase in cardiac dysfunction. We have studied the mechanisms of paclitaxel/anti-ErbB2 cardiotoxi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.02.007

    authors： Pentassuglia L,Timolati F,Seifriz F,Abudukadier K,Suter TM,Zuppinger C

    更新日期：2007-05-01 00:00:00

  • Trichostatin A affects the secretion pathways of beta and intestinal endocrine cells.

    abstract：:Histone deacetylase inhibitors (HDACi) were recently identified as having significant clinical potential in reversing β-cell functional inhibition caused by inflammation, a shared precursor of Type 1 and Type 2 diabetes. However, HDACi are highly complex and little is known of their direct effect on important cell sec...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.09.031

    authors： Tiernan AR,Champion JA,Sambanis A

    更新日期：2015-01-01 00:00:00

  • Augmenter of liver regeneration inhibits TGF-β1-induced renal tubular epithelial-to-mesenchymal transition via suppressing TβR II expression in vitro.

    abstract：:Tubular epithelial-to-mesenchymal transition (EMT) plays a crucial role in the progression of renal tubular interstitial fibrosis (TIF), which subsequently leads to chronic kidney disease (CKD) and eventually, end-stage renal disease (ESRD). We propose that augmenter of liver regeneration (ALR), a member of the newly ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.07.001

    authors： Liao XH,Zhang L,Chen GT,Yan RY,Sun H,Guo H,Liu Q

    更新日期：2014-10-01 00:00:00

  • Internalization of the hyaluronan receptor CD44 by chondrocytes.

    abstract：:Chondrocytes express CD44 as a primary receptor for the matrix macromolecule hyaluronan. Hyaluronan is responsible for the retention and organization of proteoglycan within cartilage, and hyaluronan-chondrocyte interactions are important for the assembly and maintenance of the cartilage matrix. Bovine articular chondr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4641

    authors： Aguiar DJ,Knudson W,Knudson CB

    更新日期：1999-11-01 00:00:00

  • Involvement of JNK signaling in IL4-induced M2 macrophage polarization.

    abstract：:It has been generally accepted that alternatively activated macrophages (M2), which can be induced by type 2 cytokines such as IL-4, is responsible for tissue repair. However, the function of JNK in IL-4-induced M2 macrophage polarization remains unclear. Here, we demonstrated that M0 macrophages can be polarized into...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.05.010

    authors： Hao J,Hu Y,Li Y,Zhou Q,Lv X

    更新日期：2017-08-15 00:00:00

  • Expression of the cytoskeletal-associated protein filamin in adult rat organs.

    abstract：:Filamin is a well-characterized actin-associated protein first isolated from chicken smooth muscle. Subsequently, this polypeptide and its nonmuscle homolog actin-binding protein have been shown to be expressed in avian muscle tissue, mammalian smooth muscle, mammalian macrophages and other blood cell types, as well a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1317

    authors： Brown KD,Binder LI

    更新日期：1993-12-01 00:00:00

  • Macaque trophoblast migration is regulated by RANTES.

    abstract：:In human and non-human primates, migratory trophoblasts penetrate the uterine epithelium, invade the endometrium, enter the uterine vasculature, and migrate within the arteries. The mechanisms that regulate this directional migration are unknown. We have used early gestation macaque trophoblasts to test the hypothesis...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.01.013

    authors： Thirkill TL,Lowe K,Vedagiri H,Blankenship TN,Barakat AI,Douglas GC

    更新日期：2005-05-01 00:00:00

  • Detection in BHK cells of a precursor form for lamin A.

    abstract：:Lamins are structural proteins found in the fibrous lamina underlining the nuclear envelope. In vitro translation of polyadenylated RNA or polysomes followed by immunoprecipitation with a serum raised against BHK nuclear matrix proteins showed that lamin A (72 kD) is synthesized as a high molecular weight precursor (7...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90084-9

    authors： Dagenais A,Bibor-Hardy V,Laliberté JF,Royal A,Simard R

    更新日期：1985-12-01 00:00:00

  • The zinc finger protein CG12744 is essential for differentiation and regeneration after infection in the adult Drosophila midgut.

    abstract：:Pluripotent stem cell activity is essential to maintain regeneration and homeostasis in the Drosophila midgut following environmental challenges. Although multiple pathways have been implicated in epithelial renewal, the underlying regulatory mechanisms and correlations between relevant genes and pathways remain elusi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.10.021

    authors： Liu Q,Jin LH

    更新日期：2017-12-15 00:00:00

  • Mitochondrial membrane potential and DNA stainability in human sperm cells: a flow cytometry analysis with implications for male infertility.

    abstract：:Sperm cells from control donors of proven fertility and men from barren couples were studied by conventional procedures, i.e., light microscopy as well as flow cytometry. Light microscopy analysis of semen included the measurement of spermatozoa concentration, morphology, and motility. All the men from barren couples ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4064

    authors： Troiano L,Granata AR,Cossarizza A,Kalashnikova G,Bianchi R,Pini G,Tropea F,Carani C,Franceschi C

    更新日期：1998-06-15 00:00:00

  • Cytofluorometric DNA base determination for the investigation of heterochromatin and heterochromatin amplification.

    abstract：:The measurement of chromomycin A3/DAPI fluorescence ratios is shown to allow base content determinations in eu- and heterochromatic regions of interphase cell nuclei. The base content values obtained in chromocenters and euchromatin of Scilla sibirica agree with those measured earlier [12] on the band and non-band are...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90223-9

    authors： Leemann U,Ruch F

    更新日期：1983-09-01 00:00:00

  • Conversion of dense lysosomes into light lysosomes during hepatocytic autophagy.

    abstract：:Incubation of isolated rat hepatocytes under conditions which support maximal autophagy (amino acid-free medium) caused a marked alteration in the density distribution of lysosomes in continuous metrizamide gradients (mean peak density reduced from 1.14 to 1.09 g/ml). The autophagic sequestration inhibitor 3-methylade...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90141-7

    authors： Seglen PO,Solheim AE

    更新日期：1985-04-01 00:00:00

  • Fusion-mediated implantation of band 3 into living cells. A new system to study degradation of membrane proteins.

    abstract：:Sendai virus (SV) glycoproteins were co-reconstituted with the erythrocyte membrane polypeptide designated as band 3 (B3). Fusion of the hybrid vesicles, SV-B3, with L, HeLa and Friend erythroleukemic (FEL) cells led to fusion-mediated implantation of the erythrocyte band 3 into the plasma membranes of the above cells...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90190-8

    authors： Beigel M,Loyter A

    更新日期：1983-10-01 00:00:00

  • Senescent and quiescent cell inhibitors of DNA synthesis. Membrane-associated proteins.

    abstract：:Cytoplasts derived from senescent and quiescent human diploid cells inhibit DNA synthesis initiation when fused with cells capable of proliferation. When the cytoplasts were subjected to a variety of conditions (trypsin and cycloheximide treatment and growth on fibronectin), this inhibitory activity was lost, suggesti...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90177-6

    authors： Pereira-Smith OM,Fisher SF,Smith JR

    更新日期：1985-10-01 00:00:00

  • Effects of depletion of intracellular tetrahydrobiopterin in murine erythroleukemia cells.

    abstract：:The biosynthesis of 6(R)-5,6,7,8-tetrahydrobiopterin (BH4) in murine erythroleukemia (MEL) cells is almost completely inhibited by 10 mM, 2,4-diamino-6-hydroxypyrimidine (DAHP), which targets GTP cyclohydrolase. The inhibition results in dephosphorylation of the retinoblastoma gene product, prolongation of the G1-phas...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0021

    authors： Zhuo S,Fan S,Kaufman S

    更新日期：1996-01-10 00:00:00

  • High content and high throughput screening to assess the angiogenic and neurogenic actions of mesenchymal stem cells in vitro.

    abstract：:Mesenchymal stem cells are universally regarded across many fields of medicine, as one of the most promising cell types for use in cell-based therapies. Although not yet fully understood, the therapeutic effects of these cells are largely attributed to the trophic actions of growth factors and cytokines present in the...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.12.019

    authors： Bara JJ,Turner S,Roberts S,Griffiths G,Benson R,Trivedi JM,Wright KT

    更新日期：2015-04-10 00:00:00