Abstract：
:Two SV40-transformed human cell lines, GM637, derived from a normal human subject, and GM5849, derived from a patient with ataxia-telangiectasia (A-T), were grown in increasing concentrations of the cytotoxic agent methotrexate (MTX). The GM637 line was naturally more resistant to methotrexate than was GM5849 and, over a 5-month period, became resistant even to very high concentrations (up to 100 microM). The GM5849 line became resistant to 500 nM methotrexate during the same period. However, dot blot and Southern blot analyses showed that both cell lines had amplified their dihydrofolate reductase (dhfr) genes to about the same extent, approx. 50-fold. Using the GM5849 line with amplified dhfr, we attempted to determine if interruption of DNA synthesis by hydroxyurea would cause DNA to be replicated twice within a single cell cycle, as has been reported for Chinese hamster ovary cells. No evidence for such a phenomenon was obtained.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Hahn P,Kapp LN,Painter RBdoi
10.1016/0014-4827(87)90418-6subject
Has Abstractpub_date
1987-01-01 00:00:00pages
89-94issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(87)90418-6journal_volume
168pub_type
杂志文章abstract：:Collagen VI is a survival factor for skeletal muscle produced by endomysial cells and localized in connective tissue around muscle fibers. Mutations of its genes (COL6A1, COL6A2 and COL6A3) cause two muscular disorders, Bethlem myopathy and Ullrich disease. Expression of Collagen VI is highly dynamic during developmen...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.08.006
更新日期：2008-11-15 00:00:00
abstract：:The C6 rat glioma cell line is shown to consist of a mixed population of cells which either contain vimentin (80% of the cells) or completely lack any cytoplasmic intermediate filament (IF) proteins. Subclones could be established with both phenotypes, indicating that these IF protein expression patterns represent sta...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90423-r
更新日期：1991-12-01 00:00:00
abstract：:Transforming growth factor-beta (TGF-beta) induces apoptosis in fetal rat hepatocytes. However, a subpopulation of these cells survives, concomitant with changes in phenotype, reminiscent of an epithelial-mesenchymal transition (EMT). We have previously suggested that EMT might confer cell resistance to apoptosis (Val...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.05.017
更新日期：2006-09-10 00:00:00
abstract：:The adenosine analog 5,6 dichloro-1-beta-ribofuranosyl benzimidazole (DRB) inhibits specifically mRNA production in eukaryotic cells, with concomitant alterations in pre-rRNA processing and nucleolar ultrastructure. We have focused our analysis on the effects of drug treatment on rRNA transcription through observation...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90322-1
更新日期：1983-06-01 00:00:00
abstract：:Ischemia/reperfusion (I/R) injury, one of the leading health problems in the world, is defined as a cause of cardiomyocytes death. In the present study, we investigate the role of formyl peptide receptor 1 (FPR1) in cardiomyocyte apoptosis and ventricular remodeling of I/R injury rats and the underlying mechanism invo...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.07.016
更新日期：2018-09-15 00:00:00
abstract：:T51B rat liver epithelial cells were stimulated with extracellular ATP. Changes in cytoplasmic free Ca2+ concentration [( Ca2+]i) were measured by fura-2 both in a large population of cells on coverslips in a cuvette and in single cells in a microscopic system. Extracellular ATP evoked a prompt increase in [Ca2+]i in ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90198-5
更新日期：1989-03-01 00:00:00
abstract：:Fragments of rough endoplasmic reticulum and Golgi complex purified from rat liver homogenates were injected into Xenopus oocytes and the sites of microinjection analysed by electron microscopy at different times post-injection. The in vivo incubated fragments were located by their proximity to a microinjection vacuol...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90495-7
更新日期：1986-10-01 00:00:00
abstract：:Methylated amino acids inhibit lysosomal function in cultured rat heart myocytes more effectively than the classically employed lysosomotropic weak bases. Moreover, L-leucine methyl ester (L-Leu-OMe) or L-methionine methyl ester (L-Meth-OMe) do not alter lysosomal pH or inactivate lysosomal cysteine proteinases, but d...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90690-6
更新日期：1984-09-01 00:00:00
abstract：:The relationship between topoisomerase II activity and ribosomal RNA synthesis was investigated using the antitumoral drug VM26, a specific inhibitor of topoisomerase II. For this purpose TG cells, a human tumor cell line, were cultured in the presence of 2.5 microM VM26 for 1 and 3 h; VM26 reduced the topoisomerase I...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1055
更新日期：1994-03-01 00:00:00
abstract：:RhoA activated kinases (ROCKs) are potent effectors of RhoA signaling for regulation of the cytoskeleton. ROCKs have been shown to be localized to several different subcellular locations, suggesting that its localization is context specific and regulated. However, the signaling mechanisms that control ROCK localizatio...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2011.09.009
更新日期：2011-12-10 00:00:00
abstract：:Actions of transforming growth factor-β are largely context dependent. For instance, TGF-β is growth inhibitory to epithelial cells and many tumor cell-lines while it stimulates the growth of mesenchymal cells. TGF-β also activates fibroblast cells to a myofibroblastic phenotype. In order to understand how the respons...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2015.03.001
更新日期：2015-06-10 00:00:00
abstract：:We made use of the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) method and isolated peroxisome-deficient mutant cells. TKa cells, the wild-type Chinese hamster ovary (CHO) cells, CHO-K1, that had been stably transfected with cDNA encoding Pex2p (formerly peroxisome assembly factor-1, PAF-1) were used to avoid frequent i...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3552
更新日期：1997-05-25 00:00:00
abstract：:The periderm is an epithelial layer covering the emerging epidermis in early embryogenesis of vertebrates. In the chicken embryo, an additional cellular layer, the subperiderm, occurs at later embryonic stages underneath the periderm. The questions arose what is the function of both epithelial layers and, as they are ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.06.009
更新日期：2004-10-01 00:00:00
abstract：:The fruit-fly Drosophila melanogaster harbours different types of ciliary structures: ciliary projections associated with neurons of type I and cilium-like regions (CLRs) found during male gametogenesis. The latter deserve particular attention since they are morphologically similar to vertebrate primary cilia and tran...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.08.020
更新日期：2018-10-01 00:00:00
abstract：:Procollagen I is a trimer consisting of two proalpha1(I) chains and one proalpha 2(I) chain. In certain cases of mild osteogenesis imperfecta, abnormal proalpha1(I) chains are degraded very soon after synthesis. As a consequence, the cells produce excess proalpha2(I) chains, which cannot form trimers and are not secre...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.01.029
更新日期：2004-06-10 00:00:00
abstract：BACKGROUND:CDK8 is associated with the transcriptional Mediator complex and has been shown to regulate several transcription factors implicated in cancer. As a pancreatic cancer oncogene, the role of CDK8 in cancer angiogenesis remains unclear. Here, we investigated the contribution of CDK8 in pancreatic cancer angioge...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.05.033
更新日期：2018-08-15 00:00:00
abstract：:Cellular senescence is characterized by a decline in sensitivity to growth factors resulting in cessation of cellular growth. The expression of cellular or viral oncogenes may result in the establishment of cell lines with unlimited proliferative potential ("immortalization"). A variety of viral and cellular oncogenes...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/0014-4827(90)90027-8
更新日期：1990-11-01 00:00:00
abstract：PURPOSE:Owing to the late diagnosis and frequent metastasis, ovarian cancer (OC) exhibits a high mortality rate. The study was intended to figure out the function of long non-coding RNA (lncRNA) HCP5 in OC metastasis. METHODS:Microarray analysis was conducted to probe aberrantly expressed lncRNAs in OC tissues. Artifi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112129
更新日期：2020-09-01 00:00:00
abstract：:Heterogeneous nuclear ribonucleoproteins (hnRNPs) have fundamental roles in the posttranscriptional control of gene expression. Here, we describe an hnRNP from Caenorhabditis elegans(HRP-2), which shares significant homology with mammalian hnRNP R, hnRNP Q and ACF, the essential complementation factor in ApoB mRNA edi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.04.043
更新日期：2004-08-15 00:00:00
abstract：:The altered patterns of expression of gangliosides during density-dependent growth inhibition, oncogenic transformation, and embryogenesis suggest that gangliosides, sialylated membrane glycolipids, may affect cellular proliferation and differentiation. Gangliosides of the "b" pathway of ganglioside synthesis, includi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1070
更新日期：1995-03-01 00:00:00
abstract：:Oncogenic EGFRvIII is a naturally occurring oncoprotein and is expressed in about 40-50% of human glioblastomas, particularly those that arise de novo. To understand the molecular mechanisms by which this oncoprotein alters transforming phenotypes, and since our previous work indicated that SHP-2 protein tyrosine phos...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.05.001
更新日期：2009-08-15 00:00:00
abstract：:The bone morphogenetic proteins (BMPs) are potent osteoinductive factors that accelerate osteoblast maturation, accompanied by increased cell-substrate adhesion. BMP-2 treatment of osteoblastic cells increases phosphorylation of the cytoplasmic BMP-2 signaling molecules, Smad1 and Smad5. We have previously reported th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00386-0
更新日期：2003-11-15 00:00:00
abstract：:We investigated whether cell-permeable, synthetic ceramide (C6 ceramide) could induce apoptosis in Fas-resistant Hodgkin's disease (HD)-derived cell lines. Despite strongly expressing the Fas-receptor, two of three HD-derived cell lines were resistant to Fas-mediated apoptosis. This resistance to Fas could not be attr...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4773
更新日期：2000-02-25 00:00:00
abstract：:During the polyadenylation of pre-mRNA in vitro, poly(A) binding protein II (PAB II) binds to the growing poly(A) tail, stimulating its extension. The subcellular localization of PAB II was investigated with an antibody affinity-purified from rabbit serum raised against the purified protein. Immunofluorescence microsc...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1235
更新日期：1994-09-01 00:00:00
abstract：:Chondrocytes express CD44 as a primary receptor for the matrix macromolecule hyaluronan. Hyaluronan is responsible for the retention and organization of proteoglycan within cartilage, and hyaluronan-chondrocyte interactions are important for the assembly and maintenance of the cartilage matrix. Bovine articular chondr...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4641
更新日期：1999-11-01 00:00:00
abstract：:The NF-kappaB/RelA family of transcription factors regulates inducible transcription of a large number of genes in response to diverse stimuli. Little is known, however, about the location of NF-kappaB in the cytoplasm and the transport mechanism to the nucleus. We found that NF-kappaB is associated with the actin-bin...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.12.001
更新日期：2008-03-10 00:00:00
abstract：:Several metabolic enzymes assemble into distinct intracellular structures in prokaryotes and eukaryotes suggesting an important functional role in cell physiology. The CTP-generating enzyme CTP synthase forms long filamentous structures termed cytoophidia in bacteria, yeast, fruit flies and human cells independent of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.03.042
更新日期：2017-05-15 00:00:00
abstract：:Co-cultures of lutropin receptor (LHR) positive and negative Leydig cells were used to test the hypothesis that the LHR provokes phosphorylation of the extracellular regulated kinases (ERK1/2) using intracellular and intercellular pathways. Addition of hCG to MA-10 cells (LHR positive) stimulates phosphorylation of th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.06.025
更新日期：2008-01-01 00:00:00
abstract：:Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated nucleotides has been recently proposed as a means of analyzing DNA replication and detecting proliferating cells. The presence of numerous in situ DNA strand breaks is also an inherent feature of apoptotic cells. T...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0004
更新日期：1996-01-10 00:00:00
abstract：:Tendinopathy is a common and disabling condition that is difficult to treat. The pathomolecular events behind tendinopathy remain uncertain. Micro-RNAs (miRNAs, miRs) are short non-coding RNAs that regulate gene expression and may play a role in tendinopathy development. Tenocytes were obtained from human patellar ten...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.03.008
更新日期：2019-05-15 00:00:00
