Abstract:
:The C6 rat glioma cell line is shown to consist of a mixed population of cells which either contain vimentin (80% of the cells) or completely lack any cytoplasmic intermediate filament (IF) proteins. Subclones could be established with both phenotypes, indicating that these IF protein expression patterns represent stable phenotypic markers. Absence of IF proteins in C6 subclones could consistently be correlated with an altered cell morphology and a pronounced increase in the number of actin stress fibers. In vitro translation and hybridization assays suggest the absence of vimentin to result from a block at the transcriptional level. The data indicate that subcloning of the C6 cell line on the basis of IF protein expression seems to be a reasonable approach for obtaining homogeneous C6 cell populations which may represent suitable experimental models for studies on vimentin expression and glioma cell differentiation.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Röser K,Bohn W,Giese G,Mannweiler Kdoi
10.1016/0014-4827(91)90423-rsubject
Has Abstractpub_date
1991-12-01 00:00:00pages
200-6issue
2eissn
0014-4827issn
1090-2422journal_volume
197pub_type
杂志文章abstract::Different extracellular matrix (ECM) molecules, when presented to hippocampal neurons in culture in a substrate-bound form, exert strikingly similar effects on the establishment of neuronal polarity, i.e., the growth of axon-like major neurites is favored, whereas extension of dendrite-like minor neurites is inhibited...
journal_title:Experimental cell research
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abstract::The neurohormone serotonin (5HT) triggers meiosis reinitiation in oocytes of the pelecypod molluscs Spisula solidissima and Ruditapes philippinarum. However, while Spisula oocytes complete maturation, Ruditapes oocytes arrest in metaphase I. Anti-phosphotyrosine antibody revealed that serotonin triggered an early tyro...
journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90465-8
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90154-m
更新日期:1991-01-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2016.08.023
更新日期:2016-10-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4767
更新日期:2000-02-25 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3552
更新日期:1997-05-25 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2010-07-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0123
更新日期:1996-04-10 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5312
更新日期:2001-09-10 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90437-d
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(87)90307-7
更新日期:1987-06-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90216-w
更新日期:1990-01-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.01.021
更新日期:2018-03-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(03)00340-9
更新日期:2003-11-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2010.03.010
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0021
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.04.021
更新日期:2007-08-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(87)90414-9
更新日期:1987-01-01 00:00:00
abstract::The involvement of p53 as a determinant of chemosensitivity or radiosensitivity is not well understood and is complicated by numerous contradictory reports. Here we have addressed this issue using a series of isogenic clones derived from two neuroblastoma cell lines that express wild-type p53 genes, Nub7 and IMR32. Tw...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2002.5493
更新日期:2002-04-15 00:00:00
abstract::Several metabolic enzymes assemble into distinct intracellular structures in prokaryotes and eukaryotes suggesting an important functional role in cell physiology. The CTP-generating enzyme CTP synthase forms long filamentous structures termed cytoophidia in bacteria, yeast, fruit flies and human cells independent of ...
journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2017-05-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.07.006
更新日期:2005-10-15 00:00:00
abstract::A combination of broadly expressed transcriptional activators and spatially restricted repressors are used to pattern embryos into cells of different fate. Transcriptional co-regulators are essential mediators of transcription factor function, and contribute to selective transcriptional responses in embryo development...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
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更新日期:2014-02-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1115
更新日期:1994-05-01 00:00:00
abstract::Angiogenesis plays important roles in solid tumors progression. Growth factors such as vascular endothelial growth factors (VEGFs) can induce angiogenesis and hypoxia promotes the expression of VEGFs through activating hypoxia-inducible factor 1 (HIF-1α). However, the regulation of HIF-1α still not been fully understo...
journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2019-10-01 00:00:00
abstract::Transcription factor Foxa1 plays a critical role during neural differentiation and is induced immediately after retinoic acid (RA)-initiated differentiation of pluripotent P19 embryonal carcinoma cells, correlated with the downregulated expression of pluripotency-related genes such as Nanog. To study whether Foxa1 par...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.04.020
更新日期:2014-08-15 00:00:00