当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Cellular fibronectin expression is down-regulated at the mRNA level in differentiating human intestinal epithelial cells.

Cellular fibronectin expression is down-regulated at the mRNA level in differentiating human intestinal epithelial cells.

Abstract：

:The expression of human cellular fibronectin (HFN) was analyzed in relation to the differentiation process of the human enterocyte-like cell line Caco-2. A single approximately 290-kDa form of HFN, corresponding to a 10.0-kb mRNA species, was observed in Caco-2 cells. The expression levels of both protein and mRNA were found to decrease to undetectable levels concomitantly to the differentiation process of these cells. This decline in HFN expression was correlated with the expression of sucrase-isomaltase (a specific marker of intestinal cell differentiation). These results indicate that intestinal epithelial expression of HFN is down-regulated through its mRNA levels and is correlated with the acquisition of the differentiated enterocytic phenotype.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Vachon PH,Simoneau A,Herring-Gillam FE,Beaulieu JF

doi

10.1006/excr.1995.1004

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

30-4

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(85)71004-X

journal_volume

216

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Increased mitochondrial uptake of rhodamine 123 by CDDP treatment.

    abstract：:Rhodamine 123 (R 123) is a positively charged dye at physiological pH that accumulates specifically in the mitochondria of living cells without cytotoxic effect. In the present study, the uptake of R 123 by EL-4 lymphoma cells in culture with anticancer agents was measured by flow cytometry. Changes in R 123 uptake du...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90162-2

    authors： Shinomiya N,Tsuru S,Katsura Y,Sekiguchi I,Suzuki M,Nomoto K

    更新日期：1992-01-01 00:00:00

  • High glucose increases glomerular filtration barrier permeability by activating protein kinase G type Iα subunits in a Nox4-dependent manner.

    abstract：:Hyperglycemia is a primary factor that disturbs podocyte function in the glomerular filtration process; this disturbance leads to the development of diabetic nephropathy, and ultimately, renal failure. Podocyte function may also be altered by biological agents that modify protein kinase activity, including the cGMP-ac...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.09.005

    authors： Piwkowska A,Rogacka D,Audzeyenka I,Angielski S,Jankowski M

    更新日期：2014-01-01 00:00:00

  • An optimized method for mouse liver sinusoidal endothelial cell isolation.

    abstract：:The objective of the present study was to develop an accurate and reproducible method for liver sinusoidal endothelial cell (LSEC) isolation in mice. Non-parenchymal cells were isolated using a modified two-step collagenase digestion combined with Optiprep density gradient centrifugation. LSEC were further purified us...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.10.024

    authors： Meyer J,Lacotte S,Morel P,Gonelle-Gispert C,Bühler L

    更新日期：2016-12-10 00:00:00

  • Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining.

    abstract：:On staining with a monoclonal antibody raised against microtubule-associated protein-1 (MAP-1), dot-like structures were seen in the nuclei of interphase cells, but not in those of non-cycling G0-arrested cells. Dots were also not seen in the nuclei of non-cycling senescent human cells (IMR-90). A SV40-DNA-transformed...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90062-5

    authors： Ohno T,Kako R,Sato C,Ohkawa A

    更新日期：1986-04-01 00:00:00

  • ER stress triggers apoptosis induced by Nogo-B/ASY overexpression.

    abstract：:Nogo-B/ASY has been characterized as a novel human apoptosis-inducing protein without any known apoptosis-related motifs. However, the validity of Nogo-B/ASY as a physiological apoptotic protein was recently questioned. In present research, we demonstrate that ASY overexpression contributes to ER stress and induces ap...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.02.024

    authors： Kuang E,Wan Q,Li X,Xu H,Zou T,Qi Y

    更新日期：2006-07-01 00:00:00

  • The TET2/E-cadherin/β-catenin regulatory loop confers growth and invasion in hepatocellular carcinoma cells.

    abstract：:The poor outcome of hepatocellular carcinoma (HCC) is mainly due to the development of fast growth, invasion and metastasis. The role of TET2 has been implicated in some cancer types, but its role and mechanisms in HCC remains elusive. In this study, our findings indicated that TET2 expression frequently increased in ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.01.011

    authors： Yang G,Zeng X,Wang M,Wu A

    更新日期：2018-02-15 00:00:00

  • Characterization and physiological importance of a novel cell cycle regulated protein kinase in Xenopus laevis oocytes that phosphorylates cyclin B2.

    abstract：:We have partially purified a specific cyclin B2 kinase (cyk) from prophase oocytes of Xenopus laevis after an ATP-gamma-S activation step. Phosphopeptide analysis identified Ser53 as the major in vitro phosphorylation site for cyk in cyclin B2. Using a synthetic peptide derived from cyclin B2 encompassing Ser53 (cykti...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.3436

    authors： Derua R,Stevens I,Waelkens E,Fernandez A,Lamb N,Merlevede W,Goris J

    更新日期：1997-02-01 00:00:00

  • Actin cytoskeleton organization, cell surface modification and invasion rate of 5 glioblastoma cell lines differing in PTEN and p53 status.

    abstract：:Glioblastoma cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores the relationship between the invasion capacity of 5 glioblastoma cell lines differing in p53 and PTEN status, expression of mTOR and several other marker proteins involved in cell invasion, act...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.08.013

    authors： Djuzenova CS,Fiedler V,Memmel S,Katzer A,Hartmann S,Krohne G,Zimmermann H,Scholz CJ,Polat B,Flentje M,Sukhorukov VL

    更新日期：2015-01-15 00:00:00

  • Focal adhesion kinase pp125FAK and the beta 1 integrin subunit are constitutively complexed in HaCaT cells.

    abstract：:Binding of integrins to the extracellular matrix (ECM) activates various signal transduction pathways and regulates gene expression in many cell types. Integrin-dependent cytoplasmic protein/protein interactions are necessary for activation of those signal transduction cascades. In our studies we investigated a possib...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3916

    authors： Danker K,Gabriel B,Heidrich C,Reutter W

    更新日期：1998-03-15 00:00:00

  • Demonstration of a 7-nm RNP fiber as the basic structural element in a premessenger RNP particle.

    abstract：:Balbiani ring granules in Chironomus salivary glands represent premessenger ribonucleoprotein (RNP) particles, each containing a 35- to 40-kb message for a secretory polypeptide. Their gross structure can be described as an RNP ribbon bent into a toroid. We now demonstrate that an unfolded, thin RNP fiber is observed ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90437-d

    authors： Lönnroth A,Alexciev K,Mehlin H,Wurtz T,Skoglund U,Daneholt B

    更新日期：1992-04-01 00:00:00

  • The PCNA-associated factor KIAA0101/p15(PAF) binds the potential tumor suppressor product p33ING1b.

    abstract：:The KIAA0101/p15(PAF)/OEATC-1 protein was initially isolated in a yeast two-hybrid screen for proliferating cell nuclear antigen (PCNA) binding partners, and was shown to bind PCNA competitively with the cell cycle regulator p21(WAF). PCNA is involved in DNA replication and damage repair. Using polyclonal antisera rai...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.09.020

    authors： Simpson F,Lammerts van Bueren K,Butterfield N,Bennetts JS,Bowles J,Adolphe C,Simms LA,Young J,Walsh MD,Leggett B,Fowles LF,Wicking C

    更新日期：2006-01-01 00:00:00

  • Processing of the human protocadherin Fat1 and translocation of its cytoplasmic domain to the nucleus.

    abstract：:The giant protein hFat1, a member of the cadherin superfamily, has been proposed to play roles in cerebral development, glomerular slit formation, and also to act as a tumor suppressor, but its mechanisms of action have not been elucidated. To examine functions of the transmembrane and cytoplasmic domains, they were e...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.03.006

    authors： Magg T,Schreiner D,Solis GP,Bade EG,Hofer HW

    更新日期：2005-07-01 00:00:00

  • PLCgamma participates in insulin stimulation of glucose uptake through activation of PKCzeta in brown adipocytes.

    abstract：:Based on recent studies showing that PLCgamma associates to insulin receptor, we investigated its role in insulin stimulation of glucose transport in brown adipocytes. Insulin stimulation induced rapid PLCgamma association to phosphorylated insulin receptor, and activation of PLCgamma, as assessed by the mobilization ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5570

    authors： Lorenzo M,Teruel T,Hernandez R,Kayali AG,Webster NJ

    更新日期：2002-08-15 00:00:00

  • Membrane glycoproteins involved in hepatocyte adhesion to collagen type I.

    abstract：:Liver membrane glycoproteins with affinity for immobilized collagen type I were subjected to preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by electroelution of the separated proteins. Electroeluted glycoproteins with ability to neutralize the inhibitory effect of anti-CollCA...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(88)90202-9

    authors： Gullberg D,Terracio L,Rubin K

    更新日期：1988-04-01 00:00:00

  • Heterogeneity in distribution and content of p53 in SV40-transformed mouse fibroblasts.

    abstract：:The high level and intranuclear location of the cellular phosphoprotein p53 are usually regarded as invariant features of SV40-transformed fibroblast lines. During the development of improved methods for immunocytochemical detection of p53 using SVA31 E7 mouse fibroblasts, we have observed unexpected and marked variat...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90510-0

    authors： Davis D,Wynford-Thomas D

    更新日期：1986-09-01 00:00:00

  • The IGF-1-IGF-1 receptor system modulates myocyte proliferation but not myocyte cellular hypertrophy in vitro.

    abstract：:In preliminary experiments it was established that the hypertrophic and hyperplastic responses of neonatal cardiac myocytes in culture were associated with enhanced expression of IGF-1 and IGF-1 receptors in these cells. Therefore, to determine the role of IGF-1 receptors on myocyte growth, cells were exposed to antis...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1343

    authors： Kajstura J,Cheng W,Reiss K,Anversa P

    更新日期：1994-12-01 00:00:00

  • Synchronous protein cycling in batch cultures of the yeast Saccharomyces cerevisiae at log growth phase.

    abstract：:The assumption that cells are temporally organized systems, i.e. showing relevant dynamics of their state variables such as gene expression or protein and metabolite concentration, while tacitly given for granted at the molecular level, is not explicitly taken into account when interpreting biological experimental dat...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2011.09.007

    authors： Romagnoli G,Cundari E,Negri R,Crescenzi M,Farina L,Giuliani A,Bianchi MM

    更新日期：2011-12-10 00:00:00

  • Cdc25A localisation and shuttling: characterisation of sequences mediating nuclear export and import.

    abstract：:The Cdc25 phosphatases play crucial roles in cell cycle progression by removing inhibitory phosphates from tyrosine and threonine residues of cyclin-dependent kinases. Cdc25A is an important regulator of the G1/S transition but functions also in the mitotic phase of the human cell cycle. In this paper, we investigate ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.09.012

    authors： Källström H,Lindqvist A,Pospisil V,Lundgren A,Rosenthal CK

    更新日期：2005-02-01 00:00:00

  • The effect of the cyclin-dependent kinase inhibitor olomoucine on cell cycle kinetics.

    abstract：:The effect of the cyclin-dependent (CDK) inhibitors olomoucine and roscovitine on cell kinetics was studied. To this end, nonsmall cell lung cancer (NSCLC) cell line MR65 and neuroblastoma cell line CHP-212 were pulse labeled with bromodeoxyuridine (BrdUrd) and chased in culture medium, to which various concentrations...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3700

    authors： Schutte B,Nieland L,van Engeland M,Henfling ME,Meijer L,Ramaekers FC

    更新日期：1997-10-10 00:00:00

  • Specific binding of [3H]heparin to human carcinoma SW-13 and other mammalian cells.

    abstract：:This study reports on the specific binding of [3H]heparin to human adrenocortical carcinoma cell line SW-13. Heparin binding to SW-13 cells is specific, saturable, and time- and temperature-dependent with maximum binding occurring between 90 and 120 min at 22 degrees C. Scatchard analysis revealed two classes of bindi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90099-v

    authors： Halper J

    更新日期：1990-04-01 00:00:00

  • The coxsackie- and adenovirus receptor (CAR) is an in vivo marker for epithelial tight junctions, with a potential role in regulating permeability and tissue homeostasis.

    abstract：:The coxsackie- and adenovirus receptor (CAR) is a transmembrane protein belonging to the immunoglobulin superfamily. The function of CAR as a virus receptor has been extensively analyzed, while its physiological role and expression pattern in adult tissues have remained less clear. CAR associates with epithelial tight...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.01.025

    authors： Raschperger E,Thyberg J,Pettersson S,Philipson L,Fuxe J,Pettersson RF

    更新日期：2006-05-15 00:00:00

  • Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro.

    abstract：:The goal of this study was to assess the in vitro differentiation capacity of human bone marrow-derived stem cells (hBMSCs) along retinal lineages. Mononuclear cells (MNC) were isolated from bone marrow (BM) and mobilized peripheral blood (mPB) using Ficoll-Paque density gradient centrifugation, and were sorted by mag...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.02.001

    authors： Mathivanan I,Trepp C,Brunold C,Baerlocher G,Enzmann V

    更新日期：2015-04-10 00:00:00

  • Ontogeny of basic fibroblast growth factor binding sites in mouse ocular tissues.

    abstract：:Basic fibroblast growth factor (bFGF) binding to ocular tissues has been studied by autoradiographical and biochemical approaches directly performed on sections during mouse embryonic and postnatal development. Frozen sections of embryos (9 to 18 days), newborns, and adults (1 day to 6 months) were incubated with iodi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90280-n

    authors： Fayein NA,Courtois Y,Jeanny JC

    更新日期：1990-05-01 00:00:00

  • Actinomycin D in low concentrations binds uniformly to human chromosomes.

    abstract：:The binding of actinomycin D (actD) to fixed human metaphase chromosomes was studied by using autoradiography with [3H]actD and indirect immunofluorescence with a specific anti-actD antibody. At concentrations of 0.01 and 0.1 micrograms/ml there was a uniform distribution of drug along the chromosomes as observed by b...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90373-2

    authors： Brothman AR,Lindell TJ

    更新日期：1984-03-01 00:00:00

  • NLRP3 inflammasome inhibition attenuates cisplatin-induced renal fibrosis by decreasing oxidative stress and inflammation.

    abstract：BACKGROUND/AIMS:The NOD-like receptor, pyrin domain containing-3 (NLRP3) inflammasome is involved in the progression of chronic kidney disease in several rodent models. Here, we investigated whether a specific inhibitor of NLRP3 inflammasome, MCC950, can attenuate cisplatin-induced renal fibrosis. MATERIALS:Renal fibr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.07.001

    authors： Li S,Lin Q,Shao X,Mou S,Gu L,Wang L,Zhang Z,Shen J,Zhou Y,Qi C,Jin H,Pang H,Ni Z

    更新日期：2019-10-01 00:00:00

  • Interferon-gamma engages the p70 S6 kinase to regulate phosphorylation of the 40S S6 ribosomal protein.

    abstract：:The signals generated by the IFNgamma receptor to initiate mRNA translation and generation of protein products that mediate IFNgamma responses are largely unknown. In the present study, we provide evidence for the existence of an IFNgamma-dependent signaling cascade activated downstream of the phosphatidylinositol (PI...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2003.12.021

    authors： Lekmine F,Sassano A,Uddin S,Smith J,Majchrzak B,Brachmann SM,Hay N,Fish EN,Platanias LC

    更新日期：2004-04-15 00:00:00

  • Impaired intestinal wound healing in Fhl2-deficient mice is due to disturbed collagen metabolism.

    abstract：:Four and one half LIM domain protein FHL2 participates in many cellular processes involved in tissue repair such as regulation of gene expression, cytoarchitecture, cell adhesion, migration and signal transduction. The repair process after wounding is initiated by the release of peptides and bioactive lipids. These mo...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.09.023

    authors： Kirfel J,Pantelis D,Kabba M,Kahl P,Röper A,Kalff JC,Buettner R

    更新日期：2008-12-10 00:00:00

  • Pseudolaric acid B inhibits gastric cancer cell metastasis in vitro and in haematogenous dissemination model through PI3K/AKT, ERK1/2 and mitochondria-mediated apoptosis pathways.

    abstract：:Pseudolaric acid B (PAB) is the major bioactive constituent in the root bark of Pseudolarix kaempferi and has been reported to have cytotoxicity against tumor cells. Our in vivo experiments showed that PAB could inhibit gastric cancer cell lung metastasis in a nude mouse haematogenous dissemination model. To evaluate ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.01.012

    authors： Wang D,Xin Y,Tian Y,Li W,Sun D,Yang Y

    更新日期：2017-03-01 00:00:00

  • HIF-1 and NDRG2 contribute to hypoxia-induced radioresistance of cervical cancer Hela cells.

    abstract：:Hypoxia inducible factor 1 (HIF-1), the key mediator of hypoxia signaling pathways, has been shown involved in hypoxia-induced radioresistance. However, the underlying mechanisms are unclear. The present study demonstrated that both hypoxia and hypoxia mimetic cobalt chloride could increase the radioresistance of huma...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.02.028

    authors： Liu J,Zhang J,Wang X,Li Y,Chen Y,Li K,Zhang J,Yao L,Guo G

    更新日期：2010-07-15 00:00:00

  • Reserpine-induced alterations in mucus production and calmodulin-binding proteins in a human epithelial cell line.

    abstract：:The characterization of a mucus-producing human cell line (HC-84) derived from a colon carcinoma and its response to in vitro reserpine treatment is reported. Mucous granules were demonstrated within these cells on the basis of electron microscopic examination and incorporation of [3H]glucosamine with subsequent autor...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90709-2

    authors： Brady RC,Karnaky KJ Jr,Dedman JR

    更新日期：1984-01-01 00:00:00