Pseudolaric acid B inhibits gastric cancer cell metastasis in vitro and in haematogenous dissemination model through PI3K/AKT, ERK1/2 and mitochondria-mediated apoptosis pathways.

abstract：:Resistance of cancer cells to apoptosis is dependent on a balance of multiple genetic and epigenetic mechanisms, which up-regulate efficacy of the surviving growth factor-receptor signaling pathways and suppress death-receptor signaling pathways. The Insulin-like Growth Factor-1 Receptor (IGF-1R) signaling pathway is ...

journal_title：Experimental cell research

authors： Karasic TB,Hei TK,Ivanov VN

更新日期：2010-07-15 00:00:00

abstract：:Treatment of NRK cells with TGF-alpha in the presence of serum initiates disassembly of cytoskeletal stress fibers and suppresses the synthesis of tropomyosin isoforms (TMs) 1, 2, and 3 but not TMs 4 and 5 (Cooper et al., Cancer Res. 47, 4493-4500, 1987). In order to determine how the loss of tropomyosin is induced an...

journal_title：Experimental cell research

authors： Warren RH

更新日期：1997-10-10 00:00:00

abstract：:Procollagen I is a trimer consisting of two proalpha1(I) chains and one proalpha 2(I) chain. In certain cases of mild osteogenesis imperfecta, abnormal proalpha1(I) chains are degraded very soon after synthesis. As a consequence, the cells produce excess proalpha2(I) chains, which cannot form trimers and are not secre...

journal_title：Experimental cell research

authors： Gotkin MG,Ripley CR,Lamande SR,Bateman JF,Bienkowski RS

更新日期：2004-06-10 00:00:00

abstract：:Human and rodent cells undergoing apoptosis were observed to express high levels of a novel 45,000 M(r) protein. The protein, which we have termed apoptosis specific protein (ASP), was found in Burkitt lymphoma (BL) cells and in adenovirus-transformed human and rat embryo cells induced into apoptosis by a variety of s...

journal_title：Experimental cell research

authors： Grand RJ,Milner AE,Mustoe T,Johnson GD,Owen D,Grant ML,Gregory CD

更新日期：1995-06-01 00:00:00

abstract：:Previous studies have established that Schwann cells (SC) in culture express an NGF receptor. In this study, cultures of fetal human SC were established from fetal nerves and various light microscopic (LM) and electron microscopic (EM) techniques were used to localize the NGF receptor on the SC. Results indicate that ...

journal_title：Experimental cell research

authors： Assouline JG,Pantazis NJ

更新日期：1989-06-01 00:00:00

abstract：:Previous studies have demonstrated that treatment of fibroblasts from confluent, density-inhibited cultures with 50 mM KCl solution led to the onset of DNA replication. In the present study we show that such treatment can induce aphidicolin-sensitive DNA replication in differentiated nondividing cells: dorsal root gan...

journal_title：Experimental cell research

authors： Fedorov YV,Prudovsky IA,Yegorov YE,Zelenin AV

更新日期：1993-11-01 00:00:00

abstract：:Epidermal growth factor (EGF) and transforming growth factor-beta1 (TGF-beta1), upregulated in renal diseases, have a combinational effect on epithelial-mesenchymal transformation (EMT) of renal proximal tubular cells. The aim of this study was to examine the mechanism regarding the combinational effect of EGF and TGF...

journal_title：Experimental cell research

authors： Tian YC,Chen YC,Chang CT,Hung CC,Wu MS,Phillips A,Yang CW

更新日期：2007-07-01 00:00:00

abstract：:The goal of this study was to assess the in vitro differentiation capacity of human bone marrow-derived stem cells (hBMSCs) along retinal lineages. Mononuclear cells (MNC) were isolated from bone marrow (BM) and mobilized peripheral blood (mPB) using Ficoll-Paque density gradient centrifugation, and were sorted by mag...

journal_title：Experimental cell research

authors： Mathivanan I,Trepp C,Brunold C,Baerlocher G,Enzmann V

更新日期：2015-04-10 00:00:00

abstract：:We previously demonstrated that the Tg737 gene plays a critical role in the carcinogenesis of hepatocellular carcinoma (HCC). However, few systematic investigations have focused on the biological function of Tg737 in the invasion and migration of liver cancer stem cells (LCSCs) and on its clinical significance. In thi...

journal_title：Experimental cell research

authors： You N,Tan Y,Zhou L,Huang X,Wang W,Wang L,Wu K,Mi N,Li J,Zheng L

更新日期：2017-09-15 00:00:00

abstract：:The androgen receptor (AR) plays critical roles in human prostate carcinoma progression and transformation. However, the activation of AR is regulated by co-regulators. MEIS1 protein, the homeodomain transcription factor, exhibited a decreased level in poor-prognosis prostate tumors. In this study, we investigated a p...

journal_title：Experimental cell research

authors： Cui L,Li M,Feng F,Yang Y,Hang X,Cui J,Gao J

更新日期：2014-10-15 00:00:00

abstract：:Recently, we isolated 4 distinct kinds of single chain antibody against human EGF receptor (EGFR) after screening the Keio phage display scFv library by using two methods of target-guided proximity labeling. In the current study, these monovalent scFv antibodies were converted to bivalent IgGs of humanized forms (hIgG...

journal_title：Experimental cell research

authors： Chang C,Takayanagi A,Yoshida T,Shimizu N

更新日期：2013-05-01 00:00:00

abstract：:Retinol binding protein (RBP) is the plasma transport protein of retinol. Mobilization of RBP from the liver stores is stimulated by retinol. During vitamin A deficiency, RBP secretion is specifically inhibited while its rate of biosynthesis is unaffected. As a consequence, RBP, as apoprotein, accumulates inside the e...

journal_title：Experimental cell research

authors： Tosetti F,Ferrari N,Pfeffer U,Brigati C,Vidali G

更新日期：1992-06-01 00:00:00

abstract：:It is known that freshly dissociated thyroid cell clusters form follicles in suspension culture. Thyroid epithelial cell lines, grown for many generations in vitro, fail to show colloid-containing lumina when cultured as monolayers. Several thyroid cell lines, some transformed, have been tested with respect to their a...

journal_title：Experimental cell research

authors： Nitsch L,Tacchetti C,Tramontano D,Ambesi-Impiombato FS

更新日期：1984-05-01 00:00:00

abstract：:Sensitivity of tumor cells to treatment with anticancer drugs depends on expression and function of antiapoptotic and antioxidant proteins. The goal of our study was to determine the functional role of the novel antioxidant protein Peroxiredoxin V (PrxV), in protection of human lung carcinoma cell lines against apopto...

journal_title：Experimental cell research

authors： Kropotov A,Gogvadze V,Shupliakov O,Tomilin N,Serikov VB,Tomilin NV,Zhivotovsky B

更新日期：2006-09-10 00:00:00

abstract：:CUB domains are most exclusively found in secreted proteins and in a few transmembrane proteins. These domains are approximately 110 amino acids long and have four conserved cysteines that form a β-sandwich fold. CUB domains proteins are involved in a wide range of biological functions. We have shown that CUB domains ...

journal_title：Experimental cell research

authors： Predes D,Cruz JVR,Abreu JG,Mendes FA

更新日期：2019-10-01 00:00:00

abstract：:Incubation of cultured cells at 20 degrees C blocks the transport of newly synthesized plasma membrane proteins, and the proteins accumulate intracellularly in a terminally glycosylated form. When baby hamster kidney cells are infected with the ts O45 mutant of vesicular stomatitis virus, and incubated at 20 degrees C...

journal_title：Experimental cell research

authors： de Curtis I,Howell KE,Simons K

更新日期：1988-04-01 00:00:00

abstract：:An epithelial intestinal cell line has been established from explants of fetal rat small intestine. After the 9th passage (approx. 25 population doublings) epithelial-like cells acquired the properties of a permanent cell line. The epithelial nature of this cell line, and of clone IRD 98 subsequently isolated, is supp...

journal_title：Experimental cell research

authors： Négrel R,Rampal P,Nano JL,Cavenel C,Ailhaud G

更新日期：1983-02-01 00:00:00

abstract：:Pevonedistat is a potent, selective, first-in-class NEDD8 activating enzyme inhibitor. It is now under multiple clinical trials that investigate its anticancer effect against solid tumors and leukemia. ATP-binding cassette (ABC) transporters are membrane proteins that are involved in mediating multidrug resistance (MD...

journal_title：Experimental cell research

authors： Wei LY,Wu ZX,Yang Y,Zhao M,Ma XY,Li JS,Yang DH,Chen ZS,Fan YF

更新日期：2020-03-15 00:00:00

abstract：BACKGROUND:Esophageal fibrosis is a complication of eosinophilic esophagitis (EoE) which has been attributed to both subepithelial fibrosis and to epithelial to mesenchymal transition (EMT), a process by which epithelial cells acquire mesenchymal features. Common to both causes of EoE-fibrosis is the notion that granul...

journal_title：Experimental cell research

authors： Muir AB,Lim DM,Benitez AJ,Modayur Chandramouleeswaran P,Lee AJ,Ruchelli ED,Spergel JM,Wang ML

更新日期：2013-04-01 00:00:00

abstract：:DNA topoisomerase II (topo II) is the target of many anticancer drugs and is often altered in drug-resistant cell lines. In some tumor cell lines truncated isoforms of topo IIalpha are localized to the cytoplasm. To study the localization and function of individual enzyme domains, we have epitope-tagged several fragme...

journal_title：Experimental cell research

authors： Soltermann A,Ernst A,Leroy D,Stahel RA,Gasser SM

更新日期：1999-06-15 00:00:00

abstract：:The stroma, the middle layer of the cornea, is a connective tissue making up most of the corneal thickness. The stromal extracellular matrix (ECM) consists of highly organised lamellae which are made up of tightly packed fibrils primarily composed of collagens type I and V. This layer is interspersed with keratocytes,...

journal_title：Experimental cell research

authors： Greene CA,Green CR,Dickinson ME,Johnson V,Sherwin T

更新日期：2016-09-10 00:00:00

abstract：:Lysophosphatidic acid (LPA) signaling via G protein-coupled LPA receptors mediates various biological effects in cancer cells. This study aimed to investigate the roles of LPA receptors in the regulation of cellular functions during tumor progression in osteosarcoma cells. Long-term cisplatin (CDDP)-treated MG63-C and...

journal_title：Experimental cell research

authors： Takahashi K,Fukushima K,Tanaka K,Minami K,Ishimoto K,Otagaki S,Fukushima N,Honoki K,Tsujiuchi T

更新日期：2018-08-15 00:00:00

abstract：:Mesenchymal stem cells (MSCs) derived from bone marrow (BM) represents a useful source of adult stem cells for cell therapy and tissue engineering. MSCs are present at a low frequency in the BM; therefore expansion is necessary before performing clinical studies. Fetal bovine serum (FBS) as a nutritional supplement fo...

journal_title：Experimental cell research

authors： Fani N,Ziadlou R,Shahhoseini M,Baghaban Eslaminejad M

更新日期：2016-06-10 00:00:00

abstract：:Basic fibroblast growth factor (bFGF) and transforming growth factor-beta (TGF-beta) are known to alter the migratory and proliferative capacity of endothelial cells in vitro and to stimulate angiogenesis in vivo. One mechanism by which these cytokines induce their effects may be through the regulation of integrin adh...

journal_title：Experimental cell research

authors： Enenstein J,Waleh NS,Kramer RH

更新日期：1992-12-01 00:00:00

abstract：:Growth factor-mediated stimulation of epithelial cells induces the disassembly of E-cadherin-mediated cell-cell adhesion. We found that overexpression of a disintegrin and metalloprotease 9 (ADAM9) enhanced growth factor-mediated induction of endocytosis and dynamic recycling of E-cadherin in HT29 human colon cancer c...

journal_title：Experimental cell research

authors： Hirao T,Nanba D,Tanaka M,Ishiguro H,Kinugasa Y,Doki Y,Yano M,Matsuura N,Monden M,Higashiyama S

更新日期：2006-02-01 00:00:00

abstract：:The correct functioning of epidermal growth factor receptor (EGFR), a receptor tyrosine kinase, is required for normal skin development and homeostasis. Cellular hyperproliferation induced by dysregulation of EGFR is tightly associated with structural and functional defects of hair follicles, skin lesions, and tumorig...

journal_title：Experimental cell research

authors： Chen L,Wang T,Ji X,Ding C,Liang T,Liu X,Lu J,Guo X,Kang Q,Ji Z

更新日期：2019-11-15 00:00:00

abstract：:In this study we examined by Northern blot analysis the expression of Raf-1 protooncogene in normal human peripheral blood leukocytes. Unlike thymocytes, circulating lymphocytes did not express appreciable levels of Raf-1 mRNA. In contrast, polymorphonuclear cells (PMN) had high levels of Raf-1 transcripts. Also densi...

journal_title：Experimental cell research

authors： Colotta F,Polentarutti N,Mantovani A

更新日期：1991-06-01 00:00:00

abstract：:1,25-dihydroxyvitamin D(3) (VD(3)) induces differentiation in a number of leukemia cell lines and under various conditions is able to either stimulate or inhibit nuclear factor kappa B (NF-kappaB) activity. Here we report a time-dependent biphasic regulation of NF-kappaB in VD(3)-treated HL-60 leukemia cells. After VD...

journal_title：Experimental cell research

authors： Tse AK,Wan CK,Shen XL,Zhu GY,Cheung HY,Yang M,Fong WF

更新日期：2007-05-01 00:00:00

abstract：:Upregulated ERK1/2 activity is often correlated with AKT activation during prostate cancer (PCa) progression, yet their functional relation needs elucidation. Using androgen-deprived LNCaP cells, in which ERK1/2 activation occurs in strong correlation with AKT activation, we found that AKT-mediated B-Raf regulation is...

journal_title：Experimental cell research

authors： Hong SK,Jeong JH,Chan AM,Park JI

更新日期：2013-07-15 00:00:00

abstract：:We present the first evidence that secretory organelle docking at the cell membrane can be reversed in vivo. In nondischarge (nd) mutants of Paramecium tetraurelia all trichocysts can be detached from the cell surface within 2-3 h by different means, including cytochalasin B (but not D), high cell density, or Ca2+ ion...

journal_title：Experimental cell research

authors： Pape R,Plattner H

更新日期：1990-12-01 00:00:00