Developmental regulation of elongation factor-1 delta in sea urchin suggests appearance of a mechanism for alternative poly(A) site selection in gastrulae.

abstract：:Prothymosin alpha (ProT alpha) is a nuclear protein related to cell proliferation. Its gene is highly activated during postnatal development at stages containing many proliferating but also differentiating cells. In this report, a study on ProT alpha gene expression during differentiation of human myeloid leukemic (HL...

journal_title：Experimental cell research

authors： Dosil M,Alvarez-Fernández L,Gómez-Márquez J

更新日期：1993-01-01 00:00:00

abstract：:In the present study, we have tested the influence of inhibition of endocytosis by hypertonic medium on the regulation of cell surface insulin receptors. We show that active internalization of 125I-insulin is markedly inhibited by hypertonic media and that, in parallel, cell surface invaginations are significantly dim...

journal_title：Experimental cell research

authors： Geiger D,Carpentier JL,Gorden P,Orci L

更新日期：1989-11-01 00:00:00

abstract：:Colorectal cancer (CRC) is characterized by a distinct metastatic pattern resembling chemokine-induced leukocyte trafficking. This prompted us to investigate expression, signal transduction and specific functions of the chemokine receptor CXCR4 in CRC cells and metastases. Using RT-PCR analysis and Western blotting, w...

journal_title：Experimental cell research

authors： Brand S,Dambacher J,Beigel F,Olszak T,Diebold J,Otte JM,Göke B,Eichhorst ST

更新日期：2005-10-15 00:00:00

abstract：:Osteopontin (OPN) is a multifunctional protein implicated in cellular adhesion and migration. Phosphorylation has emerged as a post-translational modification important for certain biological activities of OPN. This study demonstrates that adhesion of isolated neonatal rat osteoclasts in vitro was augmented on bovine ...

journal_title：Experimental cell research

authors： Ek-Rylander B,Andersson G

更新日期：2010-02-01 00:00:00

abstract：:Secretory phospholipase A(2) (sPLA(2)) plays important roles in mediating various cellular processes, including cell proliferation, differentiation, apoptosis, and inflammatory response. In this study, we demonstrated that a basic sPLA(2) inhibits epidermal growth factor (EGF)-induced EGF receptor activation, as deter...

journal_title：Experimental cell research

authors： Zhao S,Du XY,Chen JS,Zhou YC,Song JG

更新日期：2002-10-01 00:00:00

abstract：:Mitochondrial DNA depleted (ρ(0)) human skin fibroblasts (HSF) with suppressed oxidative phosphorylation were characterized by significant changes in the expression of 2100 nuclear genes, encoding numerous protein classes, in NF-κB and STAT3 signaling pathways, and by decreased activity of mitochondrial death pathway,...

journal_title：Experimental cell research

authors： Ivanov VN,Ghandhi SA,Zhou H,Huang SX,Chai Y,Amundson SA,Hei TK

更新日期：2011-07-01 00:00:00

abstract：:The abundant heterogeneous nuclear ribonucleoprotein M (hnRNP M) is able to associate with early spliceosomes and to influence splicing patterns of specific pre-mRNAs. Here, by a combination of immunoprecipitation and pull-down assays, we have identified PSF (polypyrimidine tract-binding protein-associated splicing fa...

journal_title：Experimental cell research

authors： Marko M,Leichter M,Patrinou-Georgoula M,Guialis A

更新日期：2010-02-01 00:00:00

abstract：:There is strong evidence that the senescent phenotype, whether induced by telomere shortening, oxidative damage, or oncogenic stimuli, is an important tumor suppressive mechanism. The melanocyte is a cell of neural crest origin that produces the pigment melanin and can develop into malignant melanomas. To understand h...

journal_title：Experimental cell research

authors： Haddad MM,Xu W,Schwahn DJ,Liao F,Medrano EE

更新日期：1999-12-15 00:00:00

abstract：:The protein kinase mammalian target of rapamycin (mTOR) is a central regulator of cell proliferation and growth, with the ribosomal subunit S6 kinase 1 (S6K1) as one of the key downstream signaling effectors. A critical role of mTOR signaling in skeletal muscle differentiation has been identified recently, and an unus...

journal_title：Experimental cell research

authors： Park IH,Erbay E,Nuzzi P,Chen J

更新日期：2005-09-10 00:00:00

abstract：:The recruitment of osteoblast progenitors involves their migration and attachment to the sites of bone formation through interactions with matrix proteins. In a time-limited cell attachment assay, coated laminin-1 inhibits the adhesion of most rat calvaria cells but attaches specifically to osteoprogenitors, as quanti...

journal_title：Experimental cell research

authors： Roche P,Rousselle P,Lissitzky JC,Delmas PD,Malaval L

更新日期：1999-08-01 00:00:00

abstract：:Molecular studies of the physiological cell death process have focused attention on the role of effector caspases as critical common elements of the lethal mechanism. Diverse death signals act afferently via distinct signaling pathways to activate these resident proenzyme molecules post-translationally. Whether this m...

journal_title：Experimental cell research

authors： Lukovic D,Komoriya A,Packard BZ,Ucker DS

更新日期：2003-10-01 00:00:00

abstract：:Caveolin-1 (Cav-1) is a principal structural protein of caveolae. Cav-1 has been implicated in cancer progression, but its precise functional roles in pituitary adenoma cells remain largely unclear. In this study, we evidenced that the level of cav-1 was elevated in the invasive pituitary adenoma. Cav-1 knockdown rest...

journal_title：Experimental cell research

authors： Yang W,Xu T,Qiu P,Xu G

更新日期：2018-06-01 00:00:00

abstract：:Indirect immunofluorescence using anti-human placental bFGF antibodies demonstrates the presence of bFGF-like reactivity in the cytoplasm and in the nucleus of adult bovine aortic endothelial cells and of normal and transformed fetal bovine aortic endothelial AG 7680 and GM 7372 cells. Biologically active immunoreacti...

journal_title：Experimental cell research

authors： Dell'Era P,Presta M,Ragnotti G

更新日期：1991-02-01 00:00:00

abstract：:We report here the first direct observation of chemotaxis to EGF by rat mammary carcinoma cells. When exposed to a gradient of EGF diffusing from a micropipette, MTLn3 cells displayed typical ameboid chemotaxis, extending a lamellipod-like protrusion and moving toward the pipette. Using a homogeneous upshift in EGF to...

journal_title：Experimental cell research

authors： Bailly M,Yan L,Whitesides GM,Condeelis JS,Segall JE

更新日期：1998-06-15 00:00:00

abstract：:High glucose concentrations associated with diabetes have been shown to cause the nonenzymatic modification of proteins. Reducing sugars covalently bind to free amine groups, undergo Amadori rearrangements, and crosslink with other glucose-modified proteins. Crosslinking of type I collagen by incubation with different...

journal_title：Experimental cell research

authors： Howard EW,Benton R,Ahern-Moore J,Tomasek JJ

更新日期：1996-10-10 00:00:00

abstract：:The KIAA0101/p15(PAF)/OEATC-1 protein was initially isolated in a yeast two-hybrid screen for proliferating cell nuclear antigen (PCNA) binding partners, and was shown to bind PCNA competitively with the cell cycle regulator p21(WAF). PCNA is involved in DNA replication and damage repair. Using polyclonal antisera rai...

journal_title：Experimental cell research

authors： Simpson F,Lammerts van Bueren K,Butterfield N,Bennetts JS,Bowles J,Adolphe C,Simms LA,Young J,Walsh MD,Leggett B,Fowles LF,Wicking C

更新日期：2006-01-01 00:00:00

abstract：:Pancreatic cancer is characterized by aggressive local invasion and early metastasis formation. Active migration of the pancreatic cancer cells is essential for these processes. We have shown previously that the pancreatic cancer cells lines CFPAC1 and IMIM-PC2 show high migratory activity, and we have investigated he...

journal_title：Experimental cell research

authors： Stock AM,Hahn SA,Troost G,Niggemann B,Zänker KS,Entschladen F

更新日期：2014-08-15 00:00:00

abstract：:The 90-kDa heat shock protein, HSP90, is an abundant molecular chaperone which functions in cellular homeostasis in prokaryotes and eukaryotes. It is well known that HSP90 plays a critical and indispensable role in regulating cell growth through modulations of various signal transduction pathways, but its roles in cel...

journal_title：Experimental cell research

authors： Zhao C,Hashiguchi A,Kondoh K,Du W,Hata J,Yamada T

更新日期：2002-05-01 00:00:00

abstract：:Polycystins 1 and 2, which are mutated in Autosomal Polycystic Kidney Disease, are involved in mechanotransduction through various mechanisms. In renal cells, polycystins not only have an important mechanotransductive role in primary cilia but are also present in intercellular contacts but their role there remains unc...

journal_title：Experimental cell research

authors： Bhoonderowa L,Hameurlaine F,Arbabian A,Faqir F,Amblard F,Coscoy S

更新日期：2016-10-15 00:00:00

abstract：:Glioblastoma cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores the relationship between the invasion capacity of 5 glioblastoma cell lines differing in p53 and PTEN status, expression of mTOR and several other marker proteins involved in cell invasion, act...

journal_title：Experimental cell research

authors： Djuzenova CS,Fiedler V,Memmel S,Katzer A,Hartmann S,Krohne G,Zimmermann H,Scholz CJ,Polat B,Flentje M,Sukhorukov VL

更新日期：2015-01-15 00:00:00

abstract：:Incubation of cultured cells at 20 degrees C blocks the transport of newly synthesized plasma membrane proteins, and the proteins accumulate intracellularly in a terminally glycosylated form. When baby hamster kidney cells are infected with the ts O45 mutant of vesicular stomatitis virus, and incubated at 20 degrees C...

journal_title：Experimental cell research

authors： de Curtis I,Howell KE,Simons K

更新日期：1988-04-01 00:00:00

abstract：:Megakaryocytes (Mks) are unique cells in the human body in that they carry a single and polyploid nucleus. It is therefore of interest to understand their nuclear ultrastructure. PML oncogenic domains (PODs) were described in several types of eukaryotic cells using human autoantibodies which recognize nuclear antigens...

journal_title：Experimental cell research

authors： Drouin A,Schmitt A,Massé JM,Cieutat AM,Fichelson S,Cramer EM

更新日期：2001-12-10 00:00:00

abstract：:Paromomycin is an aminocyclitol aminoglycoside antibiotic used for the treatment of leishmaniasis. In view of the central role of mitochondria in cellular energetics and metabolism, its effect on in vivo mitochondrial activities of Leishmania donovani promastigotes-the parasite flagellate form-was investigated. The ap...

journal_title：Experimental cell research

authors： Maarouf M,de Kouchkovsky Y,Brown S,Petit PX,Robert-Gero M

更新日期：1997-05-01 00:00:00

abstract：:Chronic high glucose levels lead to the formation of advanced glycation end-products (AGEs) as well as AGE precursors, such as methylglyoxal (MG) and glyoxal, via non-enzymatic glycation reactions in patients with diabetic mellitus. Glyoxalase 1 (GLO-1) detoxifies reactive dicarbonyls that form AGEs. To investigate th...

journal_title：Experimental cell research

authors： Kim KM,Kim YS,Jung DH,Lee J,Kim JS

更新日期：2012-01-15 00:00:00

abstract：:The role of glycans in the apical targeting of proteins in epithelial cells remains a debated question. We have expressed the mouse soluble dipeptidyl peptidase IV (DPP IV ectodomain) in kidney (MDCK) and in intestinal (Caco-2) epithelial cell lines, as a model to study the role of glycosylation in apical targeting. T...

journal_title：Experimental cell research

authors： Slimane TA,Lenoir C,Sapin C,Maurice M,Trugnan G

更新日期：2000-07-10 00:00:00

abstract：:Breast cancer is a common malignancy and a major cause of death in women worldwide. The immunomodulatory role of B cells is being increasingly recognized in autoimmune diseases and cancers. In recent years, immunotherapeutic strategies that upregulate the patient's own antitumor T cell responses have shown promise in ...

journal_title：Experimental cell research

authors： Zhu S,Wang X,Wang J,Lin J,Cong Y,Qiao G

更新日期：2017-12-01 00:00:00

abstract：:Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...

journal_title：Experimental cell research

authors： Zhang J,Harrison JS,Studzinski GP

更新日期：2011-01-01 00:00:00

abstract：:U73122, a phospholipase C inhibitor, dose dependently blocks the cGMP-induced Ca2+ release in sea urchin eggs and homogenates. U73122 inhibition was prevented by cotreatment with dithiothreitol (DTT), but DTT is ineffective when eggs or homogenates were pretreated with U73122. U73122 action is different from the other...

journal_title：Experimental cell research

authors： Lee SJ,Madden PJ,Shen SS

更新日期：1998-07-10 00:00:00

abstract：:An adenocarcinoma cell line which has the ability to arrest in G0 phase under exhausted culture conditions was established. Using the cell line, we investigated the expression of cell cycle-associated proteins including cdc2, cdk2, cyclin A, cyclin Dl, and Rb during entry into or withdrawal from the cell cycle. MAP ki...

journal_title：Experimental cell research

authors： Ohta T,Fukuda M,Wanebo HJ,Jogo K,Yamaguchi S

更新日期：1996-05-25 00:00:00

abstract：:The Saccharomyces cerevisiae ALG7 gene, which functions by initiating the dolichol pathway of protein N-glycosylation, displays properties of an early growth-response gene. To initiate studies of the involvement of ALG7 in cellular proliferation, we have now more precisely analyzed ALG7 expression in the G1 phase of c...

journal_title：Experimental cell research

authors： Pretel R,Lennon K,Bird A,Kukuruzinska MA

更新日期：1995-08-01 00:00:00