Mouse macrophage polarity and ROCK1 activity depend on RhoA and non-apoptotic Caspase 3.

abstract：:Previous studies have shown that calpains are autolytically cleaved during the disease process of mdx dystrophy, a mouse model for Duchenne muscular dystrophy, indicating that calpains may be activated and play a role in proteolysis that occurs in muscular dystrophy (J. Biol. Chem. 270(18), 10909-10914, 1995). In the ...

journal_title：Experimental cell research

authors： Spencer MJ,Tidball JG

更新日期：1996-08-01 00:00:00

abstract：:Stem cells with the ability to differentiate in specialized cell types can be extracted from a wide array of adult tissues including skeletal muscle. Here we have analyzed a population of cells isolated from skeletal muscle on the basis of their poor adherence on uncoated or collagen-coated dishes that show multi-line...

journal_title：Experimental cell research

authors： Arsic N,Mamaeva D,Lamb NJ,Fernandez A

更新日期：2008-04-01 00:00:00

abstract：:The aging proteostasis decline manifests in a failure of aging cells and organisms to properly respond to proteotoxic challenges. This proteostasis collapse has long been considered a hallmark of aging in nematodes, and has recently been shown to occur also in human cells upon entry to senescence, opening the way to e...

journal_title：Experimental cell research

authors： Meller A,Shalgi R

更新日期：2021-01-09 00:00:00

abstract：:Screening for microbial metabolites that induce transcriptional activation of the SV40 promoter resulted in the identification of two known compounds, FR901228 and trichostatin A (TSA). FR901228 is a potent antitumor drug that is currently under clinical investigation. TSA is a specific inhibitor of histone deacetylas...

journal_title：Experimental cell research

authors： Nakajima H,Kim YB,Terano H,Yoshida M,Horinouchi S

更新日期：1998-05-25 00:00:00

abstract：:The ultrastructural localization of adenylate cyclase was studied in bovine cumulus-oocyte complexes. Adenylate cyclase was observed on the plasma membrane of the oocyte and occasionally on the plasma membrane of cumulus cells. The cytochemical observations presented demonstrate that there is more adenylate cyclase in...

journal_title：Experimental cell research

authors： Kuyt JR,Kruip TA,de Jong-Brink M

更新日期：1988-01-01 00:00:00

abstract：:In S. cerevisiae the induction of heat-shock protein (HSP) synthesis is accompanied by a decrease in the cytoplasmic and vacuolar pH as determined by means of [31P]NMR spectroscopy. The relationship of HSP synthesis and acidification of the cytoplasmic pH is dose-dependent under a variety of treatments (temperature in...

journal_title：Experimental cell research

authors： Weitzel G,Pilatus U,Rensing L

更新日期：1987-05-01 00:00:00

abstract：:Plasminogen activation is observed in the human epidermis during reepithelialization of epidermal defects and under certain pathological conditions. The activation reaction depends on keratinocyte-associated plasminogen activators (PAs), which convert the ubiquitous proenzyme plasminogen into the active trypsin-like s...

journal_title：Experimental cell research

authors： Reinartz J,Schaefer B,Bechtel MJ,Kramer MD

更新日期：1996-02-25 00:00:00

abstract：:Signalling from the extracellular matrix (ECM) is a fundamental cellular input that sustains proliferation, opposes cell death and regulates differentiation. Through integrins, cells perceive both the chemical composition and physical properties of the ECM. In particular, cell behaviour is profoundly influenced by the...

journal_title：Experimental cell research

authors： Dupont S

更新日期：2016-04-10 00:00:00

abstract：:In this communication, we have studied a 72,000 mol w (p72) host protein which reacts with a mouse monoclonal antibody (PAb6) directed against antigenic determinants on the Simian virus 40 (SV40) large T antigen protein that map 5' of 0.42 map units on the viral genome. The p72 protein is an abundant basic (pI greater...

journal_title：Experimental cell research

authors： Ullrich SJ,Appella E,Mercer WE

更新日期：1988-10-01 00:00:00

abstract：:Claudins belong to a large family of transmembrane proteins that localize at tight junctions (TJs) where they play a central role in regulating paracellular transport of solutes and nutrients across epithelial monolayers. Their ability to regulate the paracellular pathway is highly influenced by changes in extracellul...

journal_title：Experimental cell research

authors： Lim TS,Vedula SR,Hui S,Kausalya PJ,Hunziker W,Lim CT

更新日期：2008-08-15 00:00:00

abstract：:Expression of the metastasis suppressor NME1 in melanoma is associated with reduced cellular motility, invasion, and metastasis, but mechanisms underlying these activities are not completely understood. Herein we report a novel mechanism through which NME1 drives formation of large, stable focal adhesions (FAs) in mel...

journal_title：Experimental cell research

authors： Leonard MK,Novak M,Snyder D,Snow G,Pamidimukkala N,McCorkle JR,Yang XH,Kaetzel DM

更新日期：2019-01-01 00:00:00

abstract：:There is increasing evidence that the hypersensitive response during plant-pathogen interactions is a form of programmed cell death. In an attempt to understand the biochemical nature of this form of programmed cell death in the cowpea-cowpea rust fungus system, proteolytic activity in extracts of fungus-infected and ...

journal_title：Experimental cell research

authors： D'Silva I,Poirier GG,Heath MC

更新日期：1998-12-15 00:00:00

abstract：:Transcription factors belonging to the basic helix-loop-helix Per-Arnt-Sim (bHLH/PAS) family control a wide variety of biological processes in mammalian and/or Drosophila. We have previously isolated bHLH/PAS Xenopus amphibian homologs of Single-minded (xSim) and aryl receptor nuclear translocator (xArnt) and characte...

journal_title：Experimental cell research

authors： Coumailleau P,Bollérot K,Lecomte C,Angelier N

更新日期：2003-07-15 00:00:00

abstract：:The microtubule cytoskeleton is known to play a role in cell structure and serve as a scaffold for a variety of active molecules in processes as diverse as motility and cell division. The literature on the role of microtubules in signal transduction, however, is marked by inconsistencies. We have investigated a well-s...

journal_title：Experimental cell research

authors： Jackman RW,Rhoads MG,Cornwell E,Kandarian SC

更新日期：2009-11-15 00:00:00

abstract：:Collagen VI is a survival factor for skeletal muscle produced by endomysial cells and localized in connective tissue around muscle fibers. Mutations of its genes (COL6A1, COL6A2 and COL6A3) cause two muscular disorders, Bethlem myopathy and Ullrich disease. Expression of Collagen VI is highly dynamic during developmen...

journal_title：Experimental cell research

authors： Braghetta P,Ferrari A,Fabbro C,Bizzotto D,Volpin D,Bonaldo P,Bressan GM

更新日期：2008-11-15 00:00:00

abstract：:This study reports on the specific binding of [3H]heparin to human adrenocortical carcinoma cell line SW-13. Heparin binding to SW-13 cells is specific, saturable, and time- and temperature-dependent with maximum binding occurring between 90 and 120 min at 22 degrees C. Scatchard analysis revealed two classes of bindi...

journal_title：Experimental cell research

authors： Halper J

更新日期：1990-04-01 00:00:00

abstract：:Progressive pathophysiologic modifications of endothelial cells are associated with aging. In vitro, endothelial cell senescence is accompanied by the failure to proliferate as well as by perturbations in gene expression. Here we show that (i) senescence enhances monoblastoid U937 cell adhesion to the endothelial mono...

journal_title：Experimental cell research

authors： Maier JA,Statuto M,Ragnotti G

更新日期：1993-09-01 00:00:00

abstract：:The development of Sjögren's syndrome (SS) is accompanied by B cell hyperproliferation and mutation. Our previous study identified aberrant expression of BST-2 (also known as Tetherin/CD317) in B cells from either the peripheral blood or infiltrated salivary glands. However, the roles of BST-2 in the regulation of B c...

journal_title：Experimental cell research

authors： Fu J,Shi H,Zhan T,Li H,Ye L,Xie L,Wang Z,Wang B,Zheng L

更新日期：2021-01-01 00:00:00

abstract：:Human 293 cells were stably transfected with a plasmid introducing a receptor for the ecdysone analog muristerone. The cells were further stably transfected with muristerone-inducible expression vectors carrying either the cDNA for the human high K(M) 5'-nucleotidase or the coding sequence of the nucleotidase linked t...

journal_title：Experimental cell research

authors： Gazziola C,Moras M,Ferraro P,Gallinaro L,Verin R,Rampazzo C,Reichard P,Bianchi V

更新日期：1999-12-15 00:00:00

abstract：:Differentiation of endoderm into intestinal epithelium is initiated at E13.5 of mouse development when there are significant changes in morphology resulting in the conversion of undifferentiated stratified epithelium into a mature epithelial monolayer. Here we demonstrate that monolayer formation is associated with th...

journal_title：Experimental cell research

authors： Abud HE,Watson N,Heath JK

更新日期：2005-02-15 00:00:00

abstract：:Invasion processes underlie or accompany several pathological processes but only a limited number of high-throughput capable phenotypic models exist to test anti-invasive compounds in vitro. We here evaluated 3D co-cultures as a high-content phenotypic screening system for fibrotic invasive processes. 3D multicellular...

journal_title：Experimental cell research

authors： Wenzel C,Otto S,Prechtl S,Parczyk K,Steigemann P

更新日期：2015-11-15 00:00:00

abstract：:It is well established that the nucleus is endowed with enzymes that are involved in lipid-dependent signal transduction pathways. Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. Previous reports have shown that the nucleus contains diacylglycerol kinases (DGKs), i.e., the...

journal_title：Experimental cell research

authors： Tabellini G,Bortul R,Santi S,Riccio M,Baldini G,Cappellini A,Billi AM,Berezney R,Ruggeri A,Cocco L,Martelli AM

更新日期：2003-07-01 00:00:00

abstract：:We have previously shown that following psoralen photoactivation (PUVA treatment) human dermal fibroblasts undergo long-term growth arrest as well as morphological and functional changes reminiscent of cellular senescence [ 1 ]. In the absence of molecular data on what constitutes normal senescence, it has been diffic...

journal_title：Experimental cell research

authors： Ma W,Wlaschek M,Brenneisen P,Schneider LA,Hommel C,Hellweg C,Sauer H,Wartenberg M,Herrmann G,Meewes C,Boukamp P,Scharffetter-Kochanek K

更新日期：2002-04-01 00:00:00

abstract：:There are a number of low-abundance small nuclear RNAs (snRNAs) in eukaryotic cells. Many of them have been assigned functions in the biogenesis of cellular RNAs, such as splicing and 3' end processing. Here, we present the sequence of Xenopus U12 snRNA and compare the secondary structures of the low-abundance U11 and...

journal_title：Experimental cell research

authors： Yu YT,Tarn WY,Yario TA,Steitz JA

更新日期：1996-12-15 00:00:00

abstract：:Mitotic PtK1 cells were treated at furrow initiation with microtubule poisons to determine the role of microtubules in the regulation of terminal cytokinetic events. The administration of anti-microtubule agents in late anaphase accelerated the rate of cytokinesis by approx. 60% as measured by changes in furrow diamet...

journal_title：Experimental cell research

authors： Hamilton BT,Snyder JA

更新日期：1983-04-01 00:00:00

abstract：:Fibronectin and alpha 1(I) and alpha 2(I) collagen proteins and RNAs are highly expressed during the growth phase of the non-transformed L6 alpha 1 rat myoblasts. When L6 alpha 1 cells from myotubes following transfer to low serum medium, the levels of fibronectin RNA decrease 8-fold, those of both alpha 2(I) transcri...

journal_title：Experimental cell research

authors： Leibovitch SA,Hillion J,Leibovitch MP,Guillier M,Schmitz A,Harel J

更新日期：1986-10-01 00:00:00

abstract：:We have evaluated the importance of the Ser/Thr protein phosphorylation and dephosphorylation for chondrogenesis in high-density chicken limb bud mesenchymal cell cultures (HDCs) by using H89, a cell-permeable protein kinase inhibitor, and okadaic acid (OA), a phosphoprotein phosphatase (PP)-specific inhibitor molecul...

journal_title：Experimental cell research

authors： Zákány R,Szucs K,Bakó E,Felszeghy S,Czifra G,Bíró T,Módis L,Gergely P

更新日期：2002-04-15 00:00:00

abstract：:Utilizing both the TET-OFF and TET-ON systems in combination with transcriptional control elements of the Tie-2 gene, we have established a series of transgenic activator and responder mice for TET-regulated endothelial cell-specific transgene expression in double transgenic mouse embryos and in adult mice. TET-regula...

journal_title：Experimental cell research

authors： Deutsch U,Schlaeger TM,Dehouck B,Döring A,Tauber S,Risau W,Engelhardt B

更新日期：2008-04-01 00:00:00

abstract：:Chronic lymphocytic leukemia (CLL) results from the uncontrolled proliferation and accumulation of B-1 cells, many of which demonstrate self-reactivity. The response of B-1 cells to mitogen after undergoing malignant transformation is still unclear. Using our established malignant B-1 cell lines derived from the NZB m...

journal_title：Experimental cell research

authors： Zhang M,Chong SY,Raveche ES

更新日期：2001-04-01 00:00:00

abstract：:Pure melanophore populations isolated from the tail skin of the tadpole, Rana catesbeiana, were mass cultured for a period of 2-3 years. All cell lines of amphibian melanophores studied exhibited growth crisis (in vitro ageing) followed by spontaneous transformation to a continuous cell line, as shown by changes in gr...

journal_title：Experimental cell research

authors： Kondo H,Ide H

更新日期：1983-11-01 00:00:00