Nuclear differentiation in Paramecium tetraurelia. Transplantation of vegetative micronuclei into early exconjugants.

Abstract:

:The micronucleus of Paramecium tetraurelia normally gives rise to the macronucleus by going through the series of nuclear events of meiosis, mitosis and fusion during sexual reproduction. By transplanting the micronucleus from vegetative cells into early exconjugants, we have persuaded the micronucleus to take a short cut to differentiate directly into a macronucleus. This demonstrates that the course of development of the micronucleus is flexible and can be altered by stage-specific cytoplasmic factors.

journal_name

Exp Cell Res

authors

Mikami K,Ng SF

doi

10.1016/0014-4827(83)90437-8

subject

Has Abstract

pub_date

1983-03-01 00:00:00

pages

25-30

issue

1

eissn

0014-4827

issn

1090-2422

pii

0014-4827(83)90437-8

journal_volume

144

pub_type

杂志文章
  • The over-expression of HAS2, Hyal-2 and CD44 is implicated in the invasiveness of breast cancer.

    abstract::Within tumors there appears to be an intricate balance between hyaluronan (HA) synthesis and degradation where the invading edges display increased HA metabolism. The metabolism of HA has not been characterized in breast cancer cell lines; therefore, this study quantitatively identifies and characterizes the enzymes r...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2005.07.026

    authors: Udabage L,Brownlee GR,Nilsson SK,Brown TJ

    更新日期:2005-10-15 00:00:00

  • S632A3, a new glutarimide antibiotic, suppresses lipopolysaccharide-induced pro-inflammatory responses via inhibiting the activation of glycogen synthase kinase 3β.

    abstract::Inflammatory mediators including inducible nitric oxide (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and Interleukin-6 (IL-6) contribute to the course of a variety of inflammatory diseases. S632A3 is a new member of the glutarimide antibiotics isolated from a cultured broth of Streptomyces hygrosc...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2012.08.008

    authors: Deng H,Zhang N,Wang Y,Chen J,Shen J,Wang Z,Xu R,Zhang J,Song D,Li D

    更新日期:2012-12-10 00:00:00

  • Pro-inflammatory cytokines: emerging players regulating HSC function in normal and diseased hematopoiesis.

    abstract::Hematopoiesis is the hierarchical process in which all lineages of blood cells are produced by self-renewing hematopoietic stem cells (HSCs) in the bone marrow (BM). While the regulatory factors that maintain proper HSC function and lineage output under normal conditions are well understood, significantly less is know...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审

    doi:10.1016/j.yexcr.2014.08.017

    authors: Mirantes C,Passegué E,Pietras EM

    更新日期:2014-12-10 00:00:00

  • Development of a chemically defined serum-free medium for differentiation of rat adipose precursor cells.

    abstract::Stromal-vascular cells from the epididymal fat pad of 4-week-old rats, when cultured in a medium containing insulin or insulin-like growth factor, IGF-I, triiodothyronine and transferrin, were able to undergo adipose conversion. Over ninety percent of the cells accumulated lipid droplets and this proportion was reduce...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(87)90412-5

    authors: Deslex S,Negrel R,Ailhaud G

    更新日期:1987-01-01 00:00:00

  • A melanoma cell line sensitive to expression of a fusion protein binding the retinoblastoma protein family pocket domain.

    abstract::An established melanoma cell line (MM96L) was transfected with selectable plasmid constructs encoding either whole SV40 large T antigen, or beta-galactosidase fusions with the retinoblastoma protein (Rb)-binding region of SV40 large T antigen and a nonbinding mutant derivative of it. Both of the beta-galactosidase fus...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1996.0071

    authors: Khan S,Collins RJ,Dunn IS

    更新日期:1996-02-25 00:00:00

  • Identification of a 55-kDa ezrin-related protein that induces cytoskeletal changes and localizes to the nucleolus.

    abstract::Normal and transformed human cells when stained for ezrin, an F-actin-binding ERM (ezrin/radixin/moesin) family protein, revealed a faint and intense immunofluorescence, respectively. Surprisingly, nuclear staining that was assigned to the nucleolus by confocal laser and immunoelectron microscopy was detected in both ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4491

    authors: Kaul SC,Kawai R,Nomura H,Mitsui Y,Reddel RR,Wadhwa R

    更新日期:1999-07-10 00:00:00

  • Colocalization of nestin and vimentin/desmin in skeletal muscle cells demonstrated by three-dimensional fluorescence digital imaging microscopy.

    abstract::During skeletal muscle development three intermediate filament proteins are expressed: nestin, vimentin, and desmin. Vimentin and desmin belong to the class III intermediate filaments and are closely related to each other, whereas nestin is a more distantly related, class VI, intermediate filament. It was previously o...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1994.1281

    authors: Sjöberg G,Jiang WQ,Ringertz NR,Lendahl U,Sejersen T

    更新日期:1994-10-01 00:00:00

  • Disruption of cytoplasmic microtubules by ultraviolet radiation.

    abstract::Ultraviolet (UV) irradiation of cultured human skin fibroblasts causes the disassembly of their microtubules. Using indirect immunofluorescence microscopy, we have now investigated whether damage to the microtubule precursor pool may contribute to the disruption of microtubules. Exposure to polychromatic UV radiation ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(91)90527-2

    authors: Zamansky GB,Perrino BA,Chou IN

    更新日期:1991-07-01 00:00:00

  • TGF-beta induces formation of F-actin cores and matrix degradation in human breast cancer cells via distinct signaling pathways.

    abstract::Transforming growth factor beta regulates many biological processes including cell motility and invasion. Podosomes are specialized F-actin rich structures found in normal cells, such as osteoclasts and macrophages. Tumor cells often form related structures called invadopodia that are thought to promote invasion and m...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2008.09.013

    authors: Mandal S,Johnson KR,Wheelock MJ

    更新日期:2008-11-15 00:00:00

  • Mitogen-independent phosphorylation of S6K1 and decreased ribosomal S6 phosphorylation in senescent human fibroblasts.

    abstract::The p70 ribosomal S6 kinase (S6K1) is rapidly activated following growth factor stimulation of quiescent fibroblasts and inhibition of this enzyme results in a G(1) arrest. Phosphorylation of the ribosomal S6 protein by S6K1 regulates the translation of both ribosomal proteins and initiation factors, leading to an inc...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2000.4965

    authors: Zhang H,Hoff H,Marinucci T,Cristofalo VJ,Sell C

    更新日期:2000-08-25 00:00:00

  • Nuclear import of p53 during Xenopus laevis early development in relation to DNA replication and DNA repair.

    abstract::The role of p53 in transcriptional activation of genes involved in cell cycle progression is well established. However, the wide range of functions attributed to this gene suggests that some of them might be unrelated to transcription. Here we investigated p53 localization and recruitment to chromatin during Xenopus e...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4570

    authors: Tchang F,Méchali M

    更新日期:1999-08-25 00:00:00

  • Thy-1, via its GPI anchor, modulates Src family kinase and focal adhesion kinase phosphorylation and subcellular localization, and fibroblast migration, in response to thrombospondin-1/hep I.

    abstract::Normal fibroblast subpopulations have differential surface expression of the GPI-linked raft protein Thy-1, which correlates with differences in cellular adhesion and migration in vitro. Thrombospondin-1 (TSP-1) induces an intermediate state of adhesion in fibroblasts and other cells which facilitates migration. TSP-1...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2006.07.029

    authors: Rege TA,Pallero MA,Gomez C,Grenett HE,Murphy-Ullrich JE,Hagood JS

    更新日期:2006-11-15 00:00:00

  • Hydrocortisone-induced increase of PDGF beta-receptor expression in a human malignant mesothelioma cell line.

    abstract::The effect of hydrocortisone (HC) on PDGF beta-receptor expression was studied in the human malignant mesothelioma cell line Mero-14. HC was found to induce a time- and dose-dependent increase of PDGF beta-receptor mRNA. Nuclear run off analysis revealed that HC induced increased transcription of the PDGF beta-recepto...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/s0014-4827(05)80074-6

    authors: Versnel MA,Bouts MJ,Langerak AW,van der Kwast TH,Hoogsteden HC,Hagemeijer A,Heldin CH

    更新日期:1992-05-01 00:00:00

  • Actin cytoskeleton organization, cell surface modification and invasion rate of 5 glioblastoma cell lines differing in PTEN and p53 status.

    abstract::Glioblastoma cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores the relationship between the invasion capacity of 5 glioblastoma cell lines differing in p53 and PTEN status, expression of mTOR and several other marker proteins involved in cell invasion, act...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2014.08.013

    authors: Djuzenova CS,Fiedler V,Memmel S,Katzer A,Hartmann S,Krohne G,Zimmermann H,Scholz CJ,Polat B,Flentje M,Sukhorukov VL

    更新日期:2015-01-15 00:00:00

  • PKC 412 sensitizes U1810 non-small cell lung cancer cells to DNA damage.

    abstract::Non-small cell lung carcinoma (NSCLC) is characterized by resistance to drug-induced apoptosis, which might explain the survival of lung cancer cells following treatment. Recently we have shown that the broad-range kinase inhibitor staurosporine (STS) reactivates the apoptotic machinery in U1810 NSCLC cells [Joseph et...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2004.12.017

    authors: Hemström TH,Joseph B,Schulte G,Lewensohn R,Zhivotovsky B

    更新日期:2005-04-15 00:00:00

  • Aberrant expression of basic fibroblast growth factor in NIH-3T3 cells alters drug resistance and gene amplification potential.

    abstract::We have investigated the genetic stability of NIH-3T3 cells transfected with sequences coding for basic fibroblast growth factor (bFGF) by determining drug resistance and gene amplification potential. Colony-forming experiments and fluctuation analyses showed that the frequency and rate of resistance to N-(phosphonace...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1994.1207

    authors: Huang A,Jin H,Wright JA

    更新日期:1994-08-01 00:00:00

  • Species-divergent regulation of human and mouse osteocalcin genes by calciotropic hormones.

    abstract::Although osteocalcin is the most abundant noncollagenous protein in bone, its role remains undefined. Recent studies have reported diametrically opposing responses in the vitamin D regulation of the mouse vs the human and rat osteocalcin genes. The aim of this study was to increase the understanding of these differenc...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2000.4912

    authors: Thomas GP,Bourne A,Eisman JA,Gardiner EM

    更新日期:2000-08-01 00:00:00

  • Sudden volume changes of the lumen of inverted thyroid follicles in suspension cultures. Use in estimation of rates of water flux through thyroid epithelium.

    abstract::Inverted thyroid follicles in suspension culture were observed using dark-field illumination and time-lapse video enhancement techniques. The lumens of many follicles undergo episodes of rapid shrinkage followed by relatively slow dilation. The extent of shrinkage ranged from 10 to 40% of the initial diameter. The dec...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(86)90447-7

    authors: Nitsch L,Wollman SH

    更新日期:1986-01-01 00:00:00

  • Characterization of the plasminogen activators and plasminogen activator inhibitors expressed by cells isolated from rabbit ligament and synovial tissues: evidence for unique cell populations.

    abstract::Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1993.1052

    authors: Murphy PG,Frank CB,Hart DA

    更新日期:1993-03-01 00:00:00

  • Histone H3 phosphorylation in human monocytes and during HL-60 cell differentiation.

    abstract::Phosphorylation of the nucleosome core histone H3 (H3) on Ser-10 is thought to be a prerequisite for chromatin condensation at mitosis. Although during interphase, cell differentiation, or mitogenic activation of quiescent cells, changes in chromatin structure that involve local chromatin condensation/decondensation a...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1998.4283

    authors: Juan G,Traganos F,Darzynkiewicz Z

    更新日期:1999-01-10 00:00:00

  • Synergistic control of keratinocyte adhesion through muscarinic and nicotinic acetylcholine receptor subtypes.

    abstract::The biological mechanisms involved in initiating, coordinating, and ultimately terminating cell-cell adhesion in the stratified epithelium are not well understood at present. This study was designed to elucidate the roles of the muscarinic M3, the nicotinic alpha3, and the mixed muscarinic-nicotinic alpha9 acetylcholi...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2003.12.010

    authors: Nguyen VT,Chernyavsky AI,Arredondo J,Bercovich D,Orr-Urtreger A,Vetter DE,Wess J,Beaudet AL,Kitajima Y,Grando SA

    更新日期:2004-04-01 00:00:00

  • The extracellular matrix in breast cancer predicts prognosis through composition, splicing, and crosslinking.

    abstract::The extracellular matrix in the healthy breast has an important tumor suppressive role, whereas the abnormal ECM in tumors can promote aggressiveness, and has been linked to breast cancer relapse, survival and resistance to chemotherapy. This review article gives an overview of the elements of the ECM which have been ...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审

    doi:10.1016/j.yexcr.2015.11.009

    authors: Robertson C

    更新日期:2016-04-10 00:00:00

  • Sendai virus and herpes virus type 1 induce apoptosis in human peripheral blood mononuclear cells.

    abstract::Recent reports suggest that several viruses, besides human immunodeficiency virus, induce apoptosis in infected cells. We report here that Sendai virus or Herpes simplex virus type 1 (HSV-1), two potent inducers of interferon-alpha, caused cell death in a consistent number of human peripheral blood mononuclear cells. ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1995.1131

    authors: Tropea F,Troiano L,Monti D,Lovato E,Malorni W,Rainaldi G,Mattana P,Viscomi G,Ingletti MC,Portolani M

    更新日期:1995-05-01 00:00:00

  • Small interfering RNA targeting ILK inhibits metastasis in human tongue cancer cells through repression of epithelial-to-mesenchymal transition.

    abstract::Integrin-linked kinase (ILK) is a multifunctional serine/threonine kinase. Accumulating evidences suggest that ILK are involved in cell-matrix interactions, cell proliferation, invasion, migration, angiogenesis and Epithelial-mesenchymal transition (EMT). However, the underlying mechanisms remain largely unknown. EMT ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2013.05.014

    authors: Xing Y,Qi J,Deng S,Wang C,Zhang L,Chen J

    更新日期:2013-08-01 00:00:00

  • Differing calcification processes in cultured vascular smooth muscle cells and osteoblasts.

    abstract::Arterial medial calcification (AMC) is the deposition of calcium phosphate mineral, often as hydroxyapatite, in the medial layer of the arteries. AMC shares some similarities to skeletal mineralisation and has been associated with the transdifferentiation of vascular smooth muscle cells (VSMCs) towards an osteoblast-l...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2019.04.020

    authors: Patel JJ,Bourne LE,Davies BK,Arnett TR,MacRae VE,Wheeler-Jones CP,Orriss IR

    更新日期:2019-07-01 00:00:00

  • Lithium inhibits terminal differentiation of erythroleukemia cells. Evidence for a pre-commitment 'priming' event.

    abstract::Lithium has been found to be a novel inhibitor of the terminal differentiation of Friend murine erythroleukemia cells. A general method for the quantitative analysis of differentiation inhibitors has been developed and used to compare the site of inhibition by lithium with that by vanadate. Lithium inhibits the commit...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(87)90012-7

    authors: Zaricznyj C,Macara IG

    更新日期:1987-02-01 00:00:00

  • Polycystins and intercellular mechanotransduction: A precise dosage of polycystin 2 is necessary for alpha-actinin reinforcement of junctions upon mechanical stimulation.

    abstract::Polycystins 1 and 2, which are mutated in Autosomal Polycystic Kidney Disease, are involved in mechanotransduction through various mechanisms. In renal cells, polycystins not only have an important mechanotransductive role in primary cilia but are also present in intercellular contacts but their role there remains unc...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2016.08.021

    authors: Bhoonderowa L,Hameurlaine F,Arbabian A,Faqir F,Amblard F,Coscoy S

    更新日期:2016-10-15 00:00:00

  • Intranuclear distribution of SV40 large T-antigen and transformation-related protein p53 in abortively infected cells.

    abstract::The intranuclear localization of SV40 T-antigen (T-Ag) and the cellular protein p53 was studied in SV40 abortively infected baby mouse kidney cells using two complementary methods of ultrastructural immunocytochemistry in combination with preferential staining of nuclear RNP components and electron microscope autoradi...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(88)90026-2

    authors: Puvion E,Duthu A,Harper F,Ehrhart JC,Viron A,May P

    更新日期:1988-07-01 00:00:00

  • Differential effects of bcl-2 on cell death triggered under ATP-depleting conditions.

    abstract::The intracellular ATP concentration decides on the onset of either apoptosis or necrosis in Jurkat cells exposed to death stimuli. Bcl-2 can block apoptotic demise, which occurs preferably under conditions of high cellular ATP levels. Here, we investigated the effects of Bcl-2 on the necrotic type of cell demise that ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2000.5059

    authors: Single B,Leist M,Nicotera P

    更新日期:2001-01-01 00:00:00

  • Effects of anti-PM-Scl 100 (Rrp6p exonuclease) antibodies on prenucleolar body dynamics at the end of mitosis.

    abstract::Prenucleolar bodies (PNBs) are transitory structures which serve as building blocks for nucleoli at the transition mitosis/interphase. The assembly of PNBs and their pathway are not clearly understood. To better understand these events, the behavior of the PNB-containing PM-Scl 100 protein was compared with that of ot...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4578

    authors: Fomproix N,Hernandez-Verdun D

    更新日期:1999-09-15 00:00:00