Abstract:
:Recent reports suggest that several viruses, besides human immunodeficiency virus, induce apoptosis in infected cells. We report here that Sendai virus or Herpes simplex virus type 1 (HSV-1), two potent inducers of interferon-alpha, caused cell death in a consistent number of human peripheral blood mononuclear cells. A careful analysis of infected cells by different techniques, such as optical and electron microscopy, DNA agarose gel electrophoresis, and cytofluorimetric analysis of DNA content, showed that cell death was of apoptotic type. Sendai virus was more apoptogenic than HSV-1, and it was further studied to understand the mechanism(s) by which it induced apoptosis. Physical (uv and heat) and chemical (beta-propiolactone) inactivation reduced or abolished the apoptogenic power of Sendai virus. The use of a novel technique, which allows the study of mitochondrial membrane potential (MMP) in intact cells by flow cytometry, showed that a decrease of MMP is concomitant with the appearance of the hypodiploid peak. These results suggest that Sendai virus and HSV-1 can be added to the list of viruses causing apoptosis, which appears to be a general mechanism occurring during viral infection.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Tropea F,Troiano L,Monti D,Lovato E,Malorni W,Rainaldi G,Mattana P,Viscomi G,Ingletti MC,Portolani Mdoi
10.1006/excr.1995.1131subject
Has Abstract,Author List Incompletepub_date
1995-05-01 00:00:00pages
63-70issue
1eissn
0014-4827issn
1090-2422pii
S0014482785711317journal_volume
218pub_type
杂志文章abstract::A monoclonal antibody specific for vimentin is described which, by immunofluorescence and immunoelectron microscopy, decorates fibrillar and/or granular structures in mitotic and early postmitotic cells but does not react with vimentin filaments of interphase stages of various cultured cells (rat vascular smooth muscl...
journal_title:Experimental cell research
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doi:10.1016/0014-4827(84)90181-2
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abstract::Multipotent mesenchymal stromal cells (MSCs) support wound healing processes. These cells express toll-like receptors (TLRs). TLRs perform important key functions when the immune system is confronted with danger signals. TLR ligation by lipopolysaccharides (LPS) activates MSCs and induces intracellular signaling casca...
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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更新日期:1990-03-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3811
更新日期:1997-12-15 00:00:00
abstract::Spinal muscular atrophy (SMA), a recessive, neuromuscular disease, is caused by a mutation or deletion in the SMN1 gene. The SMN2 gene is present in the same region of chromosome 5 and is similar in DNA sequence to SMN1 except for a T at position +6 of exon 7 that is likely the predominant functional difference betwee...
journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.12.009
更新日期:2007-03-10 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(87)90232-1
更新日期:1987-03-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.09.030
更新日期:2017-11-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90062-5
更新日期:1986-04-01 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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更新日期:2003-06-10 00:00:00
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journal_title:Experimental cell research
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更新日期:2004-04-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2000-08-25 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90047-9
更新日期:1986-06-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4530
更新日期:1999-07-10 00:00:00
abstract::The fruit-fly Drosophila melanogaster harbours different types of ciliary structures: ciliary projections associated with neurons of type I and cilium-like regions (CLRs) found during male gametogenesis. The latter deserve particular attention since they are morphologically similar to vertebrate primary cilia and tran...
journal_title:Experimental cell research
pub_type: 杂志文章
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